Summary
In this paper we summarize and discuss the modern technology and systems, studied and established by our research group, for performing the detection and special analysis incorporated with the super-high senstivity photon counting method for the study of ultraweak photon emission; for example, extra-weak bioluminescence and chemiluminescence from living cells and tissues, closely related to biochemical and biophysical processes and activities. An excellent sensitivity of the basic photon counting system, making it possible to achieve count rates in the very low range of one photoelectron per second to one per minute, allowed us to carry out in vivo as well as in vitro measurements, and analyses of ultraweak bioluminescence and chemiluminescence. Recent results concerning ultraweak photon emission from blood samples and organ homogenates of rats are presented and reviewed as one of the interesting and valuable applications of our modern technology for studying ultraweak cell and tissue radiation.
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Ando, W., Kabe, Y., Kobayashi, S., Takyu, C., Yamagishi, A., and Inaba, H., Formation of sulfinyl oxide and singlet oxygen in the reaction of thianthrene cation radical and superoxide ion. J. Am. chem. Soc.102 (1980) 4526–4528.
Boveris, A., Cadenas, E., Reiter, R., Filipkowski, M., Nakase, Y., and Chance, B., Organ chemiluminescence: noninvasive assay for oxidative radical reactions. Proc. natl Acad. Sci. USA77 (1980) 347–351.
Dasai, I. D., Fletcher, B. L., and Tappel, A. L., Fluorescent pigments from uterus of vitamin E-deficient rats. Lipids10 (1975) 307–309.
Hafeman, D. G., and Hoekstra, W. G., Lipid peroxidation in vivo during vitamin E and selenium deficiency in the rat. J. Nutr.107 (1977) 666–672.
Inaba, H., Kaneko, T., and Ichimura, T., Sensitivity consideration and comparative study on detection techniques of optical signals at ultra-low intensity. IEEE J. Quantum Electron.QE-5 (1969) 320.
Inaba, H., Shimizu, Y., and Tsuji, Y., Measurement of very weak light signals and spectra. Jap. J. appl. Phys.14, suppl. 14-1 (1975) 23–32.
Inaba, H., Shimizu, Y., Tsuji, Y., and Yamagishi, A., Photon counting spectral analyzing system of extra-weak chemi- and bioluminescence for biochemical applications. Photochem. Photobiol.30 (1979) 169–175.
Inaba, H., Yamagishi, A., Takyu, C., Yoda, B., Goto, Y., Miyazawa, T., Kaneda, T., and Saeki, A., Development of an ultra-high sensitive photon counting system and its application to biomedical measurements. Opt. Lasers in Eng.3 (1982) 125–130.
Inaba, H., Applications of measuring techniques of extremely weak light to medicine and life sciences. Kogaku (Optics)12 (1983) 166–179 (in Japanese).
Inaba, H., Takyu, C., Yoda, B., Sato, K., and Goto, Y., Detection of ultraweak light emission of human blood: intensity difference between cigarette smoker and nonsmoker. J. opt. Soc. Am.73 (1983) 1926.
Inaba, H., Yoda, B., and Saeki, A., Detection of ultraweak chemiluminescence of human urine with an intensity difference for nonsmokers and smokers. in: Tech. Dig. 1986 Annual Meeting of Opt. Soc. Am., pp 88–89. Seattle, USA, 19–25 Oct. 1986.
Khan, A. U., and Kasha, M., Chemiluminescence arising from simultaneous transitions in pairs of singlet oxygen molecules. J. Am. chem. Soc.92 (1970) 3293–3300.
Kobayashi, S., Sugioka, K., Nakano, M., Takyu, C., Yamagishi, A., and Inaba, H., Excitation of indole acetate in myeloperoxidase-H2O2 system: possible formation of indole acetate cation radical. Biochem. biophys. Res. Commun.93 (1980) 967–973.
Miyazawa, T., and Kaneda, T., Estimation of the proceeding of tissue lipid peroxidation by the measurement of chemiluminescence. J. Am. Oil Chem. Soc.57 (1980) 128A.
Miyazawa, T., Kaneda, T., Takyu, C., Yamagishi, A., and Inaba, H., Generation of singlet molecular oxygen in rat liver homogenate on adding autoxidized linseed oil. Agric. Biol. Chem.45 (1981) 1597–1601.
Miyazawa, T., and Kaneda, T., Extra-weak chemiluminescence of organ homogenate and blood in tocopherol-deficient rats. J. nutr. Sci. Vitaminol.24 (1981) 415–423.
Miyazawa, T., Kaneda, T., Takyu, C., and Inaba, H., Characteristics of tissue ultraweak chemiluminescence in rats fed with autoxidized linseed oil. J. nutr. Sci. Vitaminol.29 (1983) 53–64.
Morton, G. A., Photon counting. Appl. Opt.7 (1968) 1–10.
Nakano, M., Noguchi, T., Sugioka, K., Fukuyama, H., Sato, M., Shimizu, Y., Tsuji, Y., and Inaba, H., Spectroscopic evidence for the generation of singlet oxygen in the reduced nicotinamide adenine dinucleotide phosphate-dependent microsomal lipid peroxidation system. J. biol. Chem.250 (1975) 2404–2406.
Nakano, M., Takayama, K., Shimizu, Y., Tsuji, Y., Inaba, H., and Migita, T., Spectroscopic evidence for the generation of singlet oxygen in self-reaction of sec-peroxy radicals. J. Am. chem. Soc.98 (1976) 1974–1975.
Niki, E., Tanimura, R., Kamiya, Y., Takyu, C., and Inaba, H., Study of autoxidation by chemiluminescence (Part I), Mechanism of chemiluminescence. J. Japan Petrol. Inst.27 (1984) 15–20 (in Japanese).
Rieley, C. A., Cohen, C., and Liberman, M., Ethane evolution, a new index of lipid peroxidation. Science183 (1974) 208–210.
Shimizu, Y., Inaba, H., Kumaki, K., Mizuno, K., Hata, S., and Tomioka, S., Measuring methods for ultra-low light intensity and their application to extra-weak spontaneous bioluminescence from living tissues. IEEE Trans. Instrum. Meas.IM-22 (1973) 153–157.
Slawson, V., and Adamson, A. W., Chemiluminescent autoxidation of linolenic acid films on silica gel. Lipids11 (1976) 472–477.
Ushijima, Y., Nakano, M., Tsuji, Y., and Inaba, H., Excitation of indole analogs by phagocytosing leukocytes. Biochem. biophys. Res. Commun.82 (1978) 855–858.
Usuki, K., Kaneda, T., Yamagishi, A., Takyu, C., and Inaba, H., Estimation of oxidative deterioration of oils and foods by measurement of ultraweak chemiluminescence. J. Food Sci.44 (1979) 1573–1576.
Wright, J. R., Rumbaugh, R. C., Colby, H. D., and Miles, P. R., The relationship between chemiluminescence and lipid peroxidation in rat hepatic microsomes. Archs Biochem. Biophys.192 (1979) 344–351.
Yamagishi, A., Ohta, T., Konno, J., and Inaba, H., Visible fluorescence of liquid oxygen excited by a Q-switched Nd: YAG laser. J. opt. Soc. Am.71 (1981) 1197–1201.
Yoda, B., Abe, R., Goto, Y., Saeki, A., Takyu, C., and Inaba, H., Spontaneous chemiluminescence of smoker's blood, in: Analytical Applications of Bioluminescence and Chemiluminescence, pp. 587–590. Eds L. J. Kricka et al., Academic Press, London 1984.
Yoda, B., Goto, Y., Sato, K., Saeki, A., and Inaba, H., Ultra-weak chemiluminescence of smoker's blood. Archs envir. Health40 (1985) 148–150.
Yoshimoto, T., Yamamoto, S., Sugioka, K., Nakano, M., Takyu, C., Yamagishi, A., and Inaba, H., Studies on the tryptophan-dependent light emission by prostaglandin hydroperoxidase reaction. J. biol. Chem.255 (1980) 10199–10204.
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Inaba, H. Super-high sensitivity systems for detection and spectral analysis of ultraweak photon emission from biological cell cells and tissues. Experientia 44, 550–559 (1988). https://doi.org/10.1007/BF01953302
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DOI: https://doi.org/10.1007/BF01953302