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Chromatin fluorescence by pyronin staining

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Summary

Human, chicken and mouse cells from different tissues show a bright red-orange fluorescence of the chromatin after staining with pyronin Y. The possibility that intercalation of the dye into double helical nucleic acids accounts for this fluorescence pattern is briefly discussed.

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References

  1. H. Swift, Cytochemical techniques for nucleic acids. The nucleic acids chemistry and Biology, vol. 2. Academic Press, New York 1955.

    Google Scholar 

  2. R. D. Lillie, H. J. Coon's biological stains. Williams and Wilkins Co., Baltimore 1969.

    Google Scholar 

  3. A. G. E. Pearse, Histochemistry. 3rd. ed. Churchill, London 1968.

    Google Scholar 

  4. E. Gurr, Synthetic dyes in Biology, Medicine and Chemistry. Academic Press, New York and London 1971.

    Google Scholar 

  5. F. H. Kasten, Int. Rev. Cytol.21, 141 (1967).

    Article  CAS  PubMed  Google Scholar 

  6. J. Brachet, Q. J. microsc. Sci.94, 1 (1953).

    CAS  Google Scholar 

  7. L. Lison, Histochemie et cytochemie animales. vol. 1, Gauthier-Villers, Paris 1960.

    Google Scholar 

  8. J. E. Scott, Histochemie9, 30 (1967).

    Article  CAS  PubMed  Google Scholar 

  9. L. S. Lerman, J. Cell Comp. Physiol.64, suppl. 1 (1964).

  10. M. J. Waring, Nature219, 1320 (1968).

    Article  CAS  PubMed  Google Scholar 

  11. E. J. Gabbay and W. D. Wilson, Meth. Cell Biol.18, 351 (1978).

    Article  CAS  Google Scholar 

  12. J. B. Le Pecq and C. Paoletti, J. molec. Biol.27, 87 (1967).

    Article  Google Scholar 

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Authors and Affiliations

  1. Departmento de Citologia e Histologia, Facultad de Ciencias, Universidad Autónoma de Madrid, Mardir-34, Spain

    R. Armas Portela & J. C. Stockert

Authors
  1. R. Armas Portela
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  2. J. C. Stockert
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Additional information

This work was partially supported by a grant from the Comisión Asesora de Investigación Científica y Técnica, Spain.

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Armas Portela, R., Stockert, J.C. Chromatin fluorescence by pyronin staining. Experientia 35, 1663–1664 (1979). https://doi.org/10.1007/BF01953256

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