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Production and characterization of antibody against aflatoxin M1

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Summary

Antibody against aflatoxin M1 was obtained after immunization of rabbits with bovine serum albumin-afla M1 oxime conjugate. The antibody has greatest binding efficiency for afla M1, and was less efficient for afla B1. Cross-reaction of antibody with aflatoxin Q1, aflatoxicol, and aflatoxin B2a was weak. Aflatoxin B2, G1, and G2 and afla B1-guanine adduct showed almost no cross-reaction with the antibody. The sensitivity of the binding assay for aflatoxin M1 detection is in the range of 1–10 ng per assay. Detailed methods for the preparation of the conjugate, production of immune serum, and methods for antibody determination are described.

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Authors and Affiliations

  1. Department of Food Microbiology and Toxicology, University of Wisconsin, 53706, Madison, Wisconsin, USA

    W. O. Harder & F. S. Chu

Authors
  1. W. O. Harder
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  2. F. S. Chu
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Additional information

Supported by the College of Agricultural and Life Sciences, North Central Regional project NC-129, the University of Wisconsin-Madison, and by Public Health Service research grant number CA 15064 from the National Cancer Institute, NIH.

The authors wish to thank Dr R.C. Garner for providing aflatoxin-B-guanine adduct, and Dr Dennis H. Hseih for providing aflatoxicol and aflatoxin Q1.

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Harder, W.O., Chu, F.S. Production and characterization of antibody against aflatoxin M1. Experientia 35, 1104–1107 (1979). https://doi.org/10.1007/BF01949970

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