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Participation of microtubules and microfilaments in the transcellular biliary secretion of immunoglobulin A in primary cultures of rat hepatocytes

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Summary

The biliary secretion of immunoglobulin A (IgA) in primary hepatocyte cultures was investigated by means of immunofluorescence. The characteristic accumulation of IgA in visible bile canaliculi was found to be strongly inhibited by vinblastine and colchicine or by cytochalasin B, but its surface binding was not.

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Authors and Affiliations

  1. Physiologisch-chemisches Institut der Universität, Hoppe-Seyler-Str. 1, D-7400, Tübingen, Federal Republic of Germany

    R. Gebhardt

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  1. R. Gebhardt
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The author thanks Prof. Dr D. Mecke for his support and encouragement. The excellent technical assistance of Mrs M. Behringer and Mrs A. Schneck is gratefully acknowledged. The author is grateful to the BIOTEST Serum Institut (Frankfurt) for the kind gift of human IgA. This work was supported by the Deutsche Forschungsgemeinschaft.

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Gebhardt, R. Participation of microtubules and microfilaments in the transcellular biliary secretion of immunoglobulin A in primary cultures of rat hepatocytes. Experientia 40, 269–271 (1984). https://doi.org/10.1007/BF01947576

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  • DOI: https://doi.org/10.1007/BF01947576

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