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PSF and CMF, autocrine factors that regulate gene expression during growth and early development ofDictyostelium

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Abstract

Throughout growth and development,Dictyostelium cells secrete autocrine factors that accumulate in proportion to cell density. At sufficient concentration, these factors cause changes in gene expression. VegetativeDictyostelium cells continuously secrete prestarvation factor (PSF). The bacteria upon which the cells feed inhibit their response to PSF, allowing the cells to monitor their own density in relation to that of their food supply. At high PSF/bacteria ratios, which occur during late exponential growth, PSF induces the expression of several genes whose products are needed for cell aggregation. When the food supply has been depleted, PSF production declines, and a second density-sensing pathway is activated. Starving cells secrete conditioned medium factor (CMF), a glycoprotein of Mr 80 kDa that is essential for the development of differentiated cell types. Antisense mutagenesis has shown that cells lacking CMF cannot aggregate, and preliminary data suggest that CMF regulates cAMP signal transduction. Calculations indicate that a mechanism of simultaneously secreting and recognizing a signal molecule, as used byDictyostelium to monitor cell density, could also be used to determine the total number of cells in a tissue.

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  1. Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, 73104, Oklahoma City, Oklahoma, USA

    M. Clarke

  2. Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, Rice University, 77251, Houston, Texas, USA

    R. H. Gomer

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Clarke, M., Gomer, R.H. PSF and CMF, autocrine factors that regulate gene expression during growth and early development ofDictyostelium. Experientia 51, 1124–1134 (1995). https://doi.org/10.1007/BF01944730

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