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Preparation of isolated single cardiac cells from adult frog atrial tissue

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Summary

Isolated cardiac cells from bullforg atrial tissue can be readily prepared by digestion of intact fragments of atrial tissue with trypsin and collagenase. These isolated cells have dimensions of about 5 μm in width and range in length from 300 μm to over 500 μm. Such isolated cells may prove useful for the investigation of contractile activity of cardiac muscle at the single cell level and at the sarcomere level within the single cell.

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Authors and Affiliations

  1. Department of Physiology, University of Kansas Medical Center, College of Health Sciences and Hospital, 66103, Kansas City, Kansas, USA

    M. Tarr & J. W. Trank

Authors
  1. M. Tarr
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  2. J. W. Trank
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Additional information

This investigation was supported by U.S. Public Health Service, National Institutes of Health Grant No. HL 12426 and a Kansas Heart Association Grant-in-Aid.

We wish to thank Dr.J. Sommers of Duke University Medical Center for doing electron microscopy of the isolated cells to confirm the single cell nature of the preparations.

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Tarr, M., Trank, J.W. Preparation of isolated single cardiac cells from adult frog atrial tissue. Experientia 32, 338–340 (1976). https://doi.org/10.1007/BF01940825

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  • DOI: https://doi.org/10.1007/BF01940825

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