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Fractionation of staphylococcal enterotoxin A by isoelectric focusing

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  • Haematoligica, Immunologica
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Résumé

L'entérotoxine A a été divisée en six composants par focalisation isoélectrique. Les points isoélectriques (pI) variaient entre 6.78 et 8.60 à 25C; le composant prédominant se focalisait à un pH de 8.10. Les caractéristiques immunologiques et le poids moléculaire des 6 composants furent identiques.

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References

  1. F. S. Chu, K. Thedhani, E. J. Schantz andM. S. Bergdoll, Biochemistry5, 3281 (1966).

    Article  CAS  PubMed  Google Scholar 

  2. R. M. Avena andM. S. Bergdoll, Biochemistry6, 1474 (1963).

    Article  Google Scholar 

  3. D. Vesterberg andH. Svenson, Acta chem. scand.20, 820 (1966).

    Article  CAS  PubMed  Google Scholar 

  4. K. Weber andM. Osborn, J. biol. Chem.244, 4406 (1969).

    Article  CAS  PubMed  Google Scholar 

  5. Instruction Manual LKB 8100 Ampholine (LKB Producter AB, Stockholm-Brama Sweden).

  6. O. Ouchterlony, Arch. Kemi. Mineral. Geol. B.26, 1 (1949).

    Google Scholar 

  7. L. Spero, J. R. Warren andJ. F. Metzger, J. biol. Chem.248, 7289 (1973).

    Article  CAS  PubMed  Google Scholar 

  8. E. J. Schantz, W. G. Rossler, M. J. Woodbury, J. M. Lynch, H. M. Jacoby, S. J. Silverman, J. C. Gorman andL. Spero, Biochemistry11, 360 (1972).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Microbiology Division, Food Research Laboratories, Health Protection Branch, Department of National Health & Welfare, Tunney's Pasture, K1A 0L2, Ottawa, Ontario

    H. Robern, S. Stavric & N. Dickie

Authors
  1. H. Robern
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  2. S. Stavric
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  3. N. Dickie
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Additional information

We thank ProfessorM. S. Bergdoll, University of Wisconsin for kindly supplyingS. aureus strain 11N-165 reference toxin (A530-P100) and antitoxin A serum. We also thankR. Gagné, Y. Yano andD. Jeffrey for excellent technical assistance.

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Robern, H., Stavric, S. & Dickie, N. Fractionation of staphylococcal enterotoxin A by isoelectric focusing. Experientia 30, 1087–1089 (1974). https://doi.org/10.1007/BF01939026

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  • DOI: https://doi.org/10.1007/BF01939026

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