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Electron cytochemical demonstration of-SH groups in the synaptic vesicles of photoreceptor cells with the mixture of zinc iodide-osmium tetroxide

  • Specialia Anatomica, Histologica, Embryologica, Carcinologica
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Resumen

Se demuestra, en un estudio in vitro, que el ditioeritriol (DTE), agente reductor de grupos -S-S-aumenta, la reactividad de las vesículas sináapticas de las células fotorreceptoras de la rata para la mezcla de yoduro de zinc-tetrozido de osmio (ZIO), a 4°C durante 2 h, mientras que la N-etil-maleimida, bloquenante de grupos-SH, bloquea la reacción en los testigos y en los tratados previamente con DTE. Se concluye que ZIO a 4°C durante 2 h revela grupos -Sh en las vesiculas sinápticas estudiadas.

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Authors and Affiliations

  1. Instituto di Biología Celular, Facultad de Medicina, Universidad Nacional de Buenos Aires, Paraguay 2155, Buenos Aires, Argentina

    Amanda Pellegrino de Iraldi

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  1. Amanda Pellegrino de Iraldi
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Additional information

I am grateful to Prof.G. Jaim Etcheverry for stimulating discussions and to MissMargarita López for her skilful technical assistance.

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Pellegrino de Iraldi, A. Electron cytochemical demonstration of-SH groups in the synaptic vesicles of photoreceptor cells with the mixture of zinc iodide-osmium tetroxide. Experientia 31, 842–844 (1975). https://doi.org/10.1007/BF01938497

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  • DOI: https://doi.org/10.1007/BF01938497

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