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9,10-Dihydroergotamine: Production of antibodies and radioimmunoassay

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Summary

Antibodies against 9,10-dihydroergotamine (DHE) were produced by immunizing rabbits with a conjugate of 6-nor-6-carboxymethyl-9,10-dihydroergotamine and bovine serum albumin. A highly specific and sensitive radioimmunoassay for DHE has been developed.

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Authors and Affiliations

  1. Pharmaceutical Division, Sandoz Ltd., CH-4002, Basel, Switzerland

    J. Rosenthaler & H. Munzer

Authors
  1. J. Rosenthaler
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  2. H. Munzer
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Additional information

We are greatly indebted to Dr.T. Fehr for the synthesis of 6-nor-6-carboxymethyl-9,10-dihydroergotamine, and to Dr.E. Schreier for providing us with 9,10-dihydroergotamine-[13-3H]-base of high specific activity.

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Rosenthaler, J., Munzer, H. 9,10-Dihydroergotamine: Production of antibodies and radioimmunoassay. Experientia 32, 234–236 (1976). https://doi.org/10.1007/BF01937784

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  • DOI: https://doi.org/10.1007/BF01937784

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