Summary
By determination using fluorescence polarization measurements with 1,6-diphenyl 1,3,5-hexatriene, ergosterolreplaced pellicle and microsome membranes ofTetrahymena cells become less fluid, whereas those membranes from chimyl alcohol-fed cells are more fluid, when compared with the control native membranes.
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References
S.J. Singer, A. Rev. Biochem.43, 805 (1974).
D. Chapman and P.J. Quinn, Chem. Phys. Lipids17, 363 (1976).
Y. Nozawa and G.A. Thompson, J. Cell Biol.49, 722 (1971).
H. Iida, T. Maeda, K. Ohki, Y. Nozawa and S. Ohnishi, Biochim. biophys. Acta, in press.
H. Shimonaka and Y. Nozawa, Cell Structure and Function2, 81 (1977).
C.E. Martin, K. Hiramitsu, Y. Kitajima, Y. Nozawa, L. Skriver and G.A. Thompson, Biochemistry15, 5218 (1977).
R. Kasai, Y. Kitajima, C.E. Martin, Y. Nozawa, L. Skriver and G.A. Thompson, Biochemistry15, 5228 (1977).
G.A. Thompson and Y. Nozawa, Biochim. biophys. Acta472, 55 (1977).
Y. Nozawa, H. Fukushima and H. Iida, Biochim. biophys. Acta406, 248 (1975).
H. Fukushima, T. Watanabe and Y. Nozawa, Biochim. biophys. Acta436, 249 (1976).
G.A. Thompson and Y. Nozawa, J. Cell Biol.49, 712 (1971).
Y. Nozawa, in: Methods in Cell Biology, vol. 10, p. 105. Ed. D.M. Prescott, Academic Press, New York 1975.
M. Shinitzky and M. Inbar, J. molec. Biol.85, 603 (1974).
M. Shinitzky and Y. Barenholz, J. biol. Chem.249, 2652 (1974).
Y. Nozawa, H. Iida, H. Fukushima, K. Ohki and S. Ohnishi, Biochim. biophys. Acta367, 134 (1974).
H. Fukushima, C.E. Martin, H. Iida, Y. Kitajima, G.A. Thompson and Y. Nozawa, Biochim. biophys. Acta431, 165 (1976).
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Acknowledgments. We wish to express thanks to Central Scientific Commerce, Inc., Tokyo for permission to use an Elscint Microviscosimeter MV-Ia. This work was in part supported by grant from the Ministry of Education.
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Shimonaka, H., Fukushima, H., Kawai, K. et al. Altered microviscosity of in vivo lipid-manipulated membranes inTetrahymena pyriformis: A fluorescence study. Experientia 34, 586–587 (1978). https://doi.org/10.1007/BF01936974
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DOI: https://doi.org/10.1007/BF01936974