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Strychnine as an anticholinesterase: In vitro studies with rat brain enzymes

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La estricnna inhibe competitivamente a la acetilcolinesterasa (AChE, acetilcolina acetil-hidrolasa, E.C. 3.1.1.7) y a la colinesterasa (ChE, acilcolina acilhidrolasa, E.C. 3.1.1.8) de cerebro de rata. Se calculó un ki de 7×10−5 M para AChE y de 1.7×10−4 M para ChE cuando sus actividades enzimáticas se midieron con acetilbetametilcolina y butirilcolina, respectivamente. Se analizó la relevancia de estos hallazgos para el mecanismo de acción de dosis altas de estricnina in vivo, concluyéndose que esta acción anticolinesterásica es de escasa importancia.

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Authors and Affiliations

  1. Department of Physiology and Biophysics Faculty of Medicine, University of Chile, Casilla 6524, Santiago-4, Chile

    G. Alid, L. F. Valdés & F. J. Orrego

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  1. G. Alid
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  2. L. F. Valdés
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  3. F. J. Orrego
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Additional information

Acknowledgments. We are grateful toO. Petit for technical assistance, to Dr. B. Cassels for the chromatographic analysis of strychnine, to Dr.J. E. Allende for the loan of the Radiometer Titrator and to Cristina Maureira for her secretarial help. Supported by Grants from NIMH No. I RO3 MH 19125 CONICYT (project No. 80) and by Comisión de Investigación Cientifica, Universidad de Chile (Project No. 95).

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Alid, G., Valdés, L.F. & Orrego, F.J. Strychnine as an anticholinesterase: In vitro studies with rat brain enzymes. Experientia 30, 266–268 (1974). https://doi.org/10.1007/BF01934819

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