Abstract
It is thought that impairment, of energy metabolism that results in deterioration of membrane function, leading to loss of the Mg2+ block on NMDA receptors, and allowing persistent activation of these receptors by glutamate, might be a cause of neuronal death in neurodegenerative disorders. Studies in rodents using mitochondrial respiratory chain toxins, such as aminooxyacetic acid, 1-methyl-4-phenylpyridinium, malonic acid and 3-nitropropionic acid, suggest that such processes may indeed be involved in neurotoxicity. Striatal and nigral degeneration induced by mitochondrial toxins in rodents resembles the neuropathology seen in humans suffering from Huntington's or Parkinson's disease, and can be prevented either by decortication or by NMDA receptor antagonists. Such experimental observations suggest that glutamate may be involved in neuronal death leading to neurodegenerative disorders in humans. If so, glutamate antagonists may offer a therapeutic approach for retarding the progression of these disabling disorders.
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Albin, R. L., and Greenamyre, J. T., Alternative excitotoxic hypothesis. Neurology42 (1992) 733–738
Alston, T. A., Mela, L., and Bright, H. J., 3-Nitropropionate, the toxic substance of Indigofera, is a suicide inactivator of succinate dehydrogenase. Proc. natl. Acad. Sci USA74 (1977) 3767–3771.
Beal, M. F., Brouillet, E., Jenkins, B., Ferrante, R. J., Kowall, N. W., Miller, J. M., Storey, E., Strivastava, R., Rosen, B. R., and Hyman, B. T., Neurochemical and histologic characterization of striatal excitotoxic lesions produced by the mitochondrial toxin 3-nitropropionic acid. J. Neurosci.13 (1993) 4181–4192.
Beal, M. F., Brouillet, E., Jenkins, B., Henshaw, R., Rosen B., and Hyman, B. T., Age-dependent striatal excitotoxic lesions produced by the endogenous mitochondrial inhibitor malonate. J. Neurochem.61 (1993) 1147–1150.
Beal, M. F., Brouillet, E., Srivastava, R., de Socarraz, H., Jenkins, B., Rosen, B., and Hyman, B. T., Both local and systemic administration of the mitochondrial toxin sodium azide result in striatal lesions by an excitotoxic mechanism. Neurology43 (1993) A407.
Beal, M. F., Hyman, B. T., and Koroshetz, W., Do defects in mitochondrial energy metabolism underlie the pathology of neurodegenerative diseases? Trends Neurosci.16 (1993) 125–131.
Beal, M. F., Kowall, N. W., Ellison, D. W., Mazurek, M. F., Swartz, K. J., and Martin, J. B., Replication of the neurochemical characteristics of Huntington's disease by quinolinic acid. Nature321 (1986) 168–171.
Beal, M. F., Swartz, K. J., Hyman, B. T., Storey, E., Finn, S. F., and Koroshetz, W., Aminooxyacetic acid results in excitotoxic lesions by a novel indirect mechanism. J. Neurochem.57 (1991) 1068–1073.
Bowling, A. C., Mutisya, E. M., Walker, L. C., Price, D. L., Cork, L. C., and Beal, M. F., Age-dependent impairment of mitochondrial function in primate brain. J Neurochem.60 (1993) 1964–1967.
Brennan, W. A., Bird, E. D., and Aprillo, V. R., Regional mitochondrial metabolism in Huntington's disease brain. J. Neurochem.44 (1985) 1448–1450.
Brouillet, E., and Beal, M. F., NMDA antagonists partially protect against MPTP induced neurotoxicity in mice. Neuro report4 (1993) 387–390.
Brouillet, E., Jenkins, B. G., Hyman, B. T., Ferrante, R. J., Kowall, N. W., Srivastava, R., Roy, D. S., Rosen, B. R., and Beal, M. F., Age-dependent vulnerability of the striatum to the mitochondrial toxin 3-nitropropionic acid. J. Neurochem.60 (1993) 356–359.
Brouillet, E. P., Shinobu, L., McGarvey, U., and Beal, M. F., Manganese injection into the rat striatum produces excitotoxic lesions by impairing energy metabolism. Exp. Neurol.120 (1993) 89–94.
Chan, P., Langston, J. W., and Di Monte, D., Relationship between the effects of MPTP on energy metabolism and levels of L-aspartate and L-glutamate in the mouse brain. Soc. Neurosci. Abstr.18 (1992) 1103.
Corsini, G. U., Vaglini, F., Fornai, F., Saginario, A., and Zuddas, A., (+)MK 801 prevents dopaminergic perikarya damage in MPTP and acetaldehyde-treated mice. Posters Neurosci.1 (1991) 33–36.
Dalia, A., Neff, N. H., and Hadjiconstantinou, M., GM1 ganglioside improves dopaminergic markers of rat mesencephalic cultures treated with MPP+. J. Neurosci.13 (1993) 3104–3111.
Dawson, V. L., Dawson, T. M., London, E. E., Bredt, D. S., and Snyder, S. H., Nitric oxide mediates glutamate neurotoxicity in primary cortical cultures. Proc. natl Acad. Sci. USA88 (1991) 6368–6371.
Di Monte, D., Jewell, S. A., Ekstrom, C., Sandy, M. S., and Smith, M. T., 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridine (MPP+) cause rapid ATP depletion in isolated hepatocytes. Bioch. biophys. Res. Commun.137 (1986) 310–315.
Drapier, J.-C., and Hibbs, J. B., Differentiation of murine macrophages to express nonspecific cytotoxicity for tumor cells results in L-arginine-dependent inhibition of mitochondrial iron-sulfur enzymes in the macrophage effect cells. J. Immunol.140 (1988) 2829–2838.
Finiels-Marlier, F., Marini, A. M., Williams, P., and Paul, S. M., The N-methyl-D-aspartate antagonist MK-801 fails to protect dopaminergic neurons from 1-methyl-4-phenylpyridinium toxicity in vitro. J. Neurochem.60 (1993) 1968–1971.
Freese, A., Finklestein, S. P., and DiFiglia, M., Basis fibroblast growth factor protects striatal neurons in vitro from NMDA-receptor mediated excitotoxicity. Brain Res.575 (1993) 351–355.
Garthwaite, J., Southam, E., and East, S. J., Glutamate receptors, nitric oxide, and cyclic GMP, in: Excitatory Amino Acids and Second Messenger Systems, pp. 87–101. Eds V. I. Teichberg and L. Turski. Springer, Berlin 1991.
Gould, D. H., and Gustine, D. L., Basal ganglia degeneration, myelin alterations, and enzyme inhibition in mice by the plant toxin 3-nitropropanoic acid. Neuropath. appl. Neurobiol.8 (1982) 377–393.
Greene, J. G., Porter, R. H. P., Eller, R. V., and Greenamyre, J. T., Inhibition of succinate dehydrogenase by malonic acid produces an “excitotoxic” lesion in rat striatum. J. Neurochem.61 (1993) 1151–1154.
Hadjiconstantinou, M., Rossetti, Z. L., Paxton, R. C., and Neff, N. H., Administration of GM1 ganglioside restores the dopamine content in striatum after chronic treatment with MPTP. Neuropharmacology25 (1986) 1075–1077.
Heikkila, R. E., Nicklas, W. J., Vyas, I., and Duvoisin, R. C., Dopaminergic toxicity of rotenone and the 1-methyl-4 phenylpyridinium ion after their stereotaxic administration to rats: implication for mechanism of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine toxicity. Neurosci. Lett.62 (1985) 389–394.
Hibbs, J. B., Overview of cytotoxic mechanisms and defense of the intracellular environment against microbes, in: The Biology of Nitric Oxide — Enzymology, Biochemistry and Immunology, pp. 201–206. Eds S. Moncada, M. A. Marletta, J. B. Hibbs, and E. A. Higgs. Portland Press, London and Chapel Hill 1992.
Hibbs, J. B., Taintor, R. R., and Vavrin, Z., Macrophage cytotoxicity: role for L-arginine deiminase activity and imino nitrogen oxidation to nitrite. Science235 (1987) 473–476.
Higashi, H., and Yamagata, T., Mechanism for ganglioside-mediated modulation of a calmodulin-dependent enzyme. J. biol. Chem.267 (1992) 9839–9843.
Higgins, D. S., and Greenamyre, J. T., Dihydrorotenone binding in Alzheimer's disease: a preliminary study. Neurology43 (1993) A251.
Ikeda, J., Kashimura, J., Morita, I., and Murota, S., Endogenous nitric oxide inhibits glutamate-induced calcium influx exclusively in the NADPH diaphorase containing neurons. J. cereb. Blood Flow Metab.13 (1993) S39.
Izumi, Y., Benz, A. M., Clifford, B. B., and Zorumski, C. F., Neurotoxic effects of sodium nitroprusside in rat hippocampal slices. Exp. Neurol.121 (1993) 14–23.
Jenner, P., Schapira, A. H. V. and Marsden, C. D., New insights into the cause of Parkinson's disease. Neurology42 (1992) 2241–2250.
Koroshetz, W. J., Jenkins, B., Rosen, B., Beal, M. F., Ubiquinone lowers occipital lactate levels in patients with Huntington's disease. Neurology43 (1993) A334.
Kharlamov, A., Guidotti, A., Costa, E., Hayes, R., and Armstrong, D., Semisynthetic sphingolipids prevent protein kinase C translocation and neuronal damage in the perifocal area following a photochemically induced thrombotic brain cortical lesion. J. Neurosci.13 (1993) 2483–2494.
Kupsch, A., Löschmann, P.-A., Sauer, H., Arnold, H., Renner, P., Pufal, D., Burg, M., Wachtel, H., ten Bruggencate, G., and Oertel, W. H., Do NMDA receptor antagonists protect against MPTP-toxicity? Biochemical and immunocytochemical analyses in black mice. Brain Res.592 (1992) 74–83.
Lange, K. W., Löschmann, P.-A., Sofic, E., Burg, M., Horowski, R., Kalveram, D. T., Wachtel, H., Riederer, P., The competitive NMDA antagonist CPP protects substantia nigra from MPTP-induced degeneration in primates. Naunyn-Schmiedeberg's Arch. Pharmac.348 (1993) 569–575.
Lange, K. W., Youdim, M. B. H., and Riederer, P., Neurotoxicity and neuroprotection in Parkinson's disease. J. neural Transm. S38 (1992) 27–44.
Lei, S. Z., Pan, Z.-H., Aggarwal, S. K., Chen, H.S. V., Hartman, J., Sucher, N. J., and Lipton, S. A., Effect of nitric oxide production on the redox modulatory site of the NMDA receptor-channel complex. Neuron8 (1992) 1087–1099.
Lipartiti, M., Lazzaro, A., and Manev, H., Ganglioside derivative LIGA 20 reduces NMDA neurotoxicity in neonatal rat brain. Neuroreport3 (1992) 919–921.
Lipton, S. A., Choi, Y.-B., Pan Z.-H., Lei, S. Z., Chen, H.-S. V., Sucher, N. J., Loscalzo, J., Singel, D. J., and Stamler, J. S., A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitroso-compounds. Nature364 (1993) 626–632.
Loiacono, R. E., and Beart, P. M., Hippocampal lesions induced by microinjection of the nitric oxide donor nitroprusside. Eur. J. Pharmac.216 (1993) 331–333.
Ludolph, A. C., He, F., Spencer, P. S., Hammerstad, J., and Sabri, M., 3-Nitropropionic acid — Exogenous animal neurotoxin and possible human striatal toxin. Can. J. Neurol. Sci.18 (1991) 492–498.
Ludolph, A. C., Seelig, M. O., Ludolph, A., Novitt, P., Allen, C. M., Spencer, P. S., and Sabri, M. I., 3-Nitropropionic acid decreases cellular energy levels and causes neuronal degeneration in cortical explants. Neurodegeneration1 (1992) 21–28.
Manev, H., Favron, M., Vicini, S., Guidotti, A., and Costa, E., Glutamate-induced neuronal death in primary cultures of cerebellar granule cells: protection by synthetic derivatives of endogenous sphingolipids. J. Pharmac. exp. Ther.252 (1990) 419–427.
Manev, H., Guidotti, A., and Costa, E., Protection by gangliosides against glutamate excitotoxicity. Adv. Lipid Res.25 (1993) 269–288.
Manzoni, O., and Bockaert, J., Nitric oxide synthase activity endogenously modulates NMDA receptors. J. Neurochem.61 (1993) 368–370.
Mattson, M. P., Zhang, Y., and Bose, S., Growth factors prevent mitochondrial dysfunction loss of calcium homeostasis, and cell injury, but not ATP depletion in hippocampal neurons deprived of glucose. Exp. Neurol.121 (1993) 1–13.
McMaster, O. G., Du, F., French, E. D., and Schwarcz, R., Focal injection of aminooxyacetic acid produces seizures and lesions in rat hippocampus: evidence for mediation by NMDA receptors. Exp. Neurol.113 (1991) 378–385.
Mihatsch, W., Russ, H., and Przuntek, H., Intracerebroventricular administration of simultaneously administered nomifensine, deprenyl, and 1-t-butyl-4, 4-diphenylpiperdine. J. Neural Transm.71 (1988) 177–188.
Michel, P. P., and Agid, Y., The glutamate antagonist, MK-801, does not prevent dopaminergic cell death induced by the 1-methyl-4-phenylpyridinium ion (MPP+) in rat dissociated mesencephalic cultures. Brain Res.597 (1992) 233–240.
Miller, R. J., Murphy, S. N., and Glaum, S. R., Neuronal Ca2+ channels and their regulation by excitatory amino acids. Ann. New York Acad. Sci. 568 (1989) 149–158.
Nakata, N., Kato, H., and Kogure, K., Protective effects of basic fibroblast growth factor against hippocampoil neuronal damage following cerebral ischemia in the gerbil. Brain Res.605 (1993) 354–356.
Nicklas, W. J., Vyas, I., and Heikkila, R. E., Inhibition of NADH-linked oxidation in brain mitochondria by MPP+, a metabolite of the neurotoxin MPTP. Life Sci. 36 (1985) 2503–2508.
Nicotera, P., Bellomo, G., and Orrenius, S., Calcium-mediated mechanisms in chemically induced cell death. Annu. Rev. Pharmax. Toxic.32 (1992) 449–470.
Nowicki, J. P., Duval, H., Poignet, H., and Scatton, B., Nitric oxide mediates neuronal death after focal cerebral ischemia in the mouse. Eur. J. Pharmac.204 (1991) 339–340.
Nozaki, K., Finklestein, S. P., and Beal, M. F., Basic fibroblast growth factor protects against hypoxia-ischemia and NMDA neurotoxicity in neonatal rats. J. cereb. Blood Flow Metab.13 (1993) 221–228.
Novelli, A., Reilly, J. A., Lysko, P. G., Henneberry, R. C., Glutamate becomes neurotoxic via the N-methyl-D-aspartate receptor when intracellular energy levels are reduced. Brain Res.451 (1988) 205–212.
Olney, J. W., Excitatory amino acids and neuropsychiatric disorders. Biol. Psychiatry26 (1989) 505–525.
Otto, D. and Unsicker, K., Basic FGF reverses chemical and morphological deficits in the nigrostriatal system of MPTP-treated mice. J. Neurosci.10 (1990) 1912–1921.
Parker, W. D., Boyson, S. J., Luder, A. S., and Parks, J. K., Evidence for a defect in NADH: ubiquinone oxidoreductase (complex I) in Huntington's disease. Neurology40 (1990) 1231–1234.
Parker, W. D., Boyson, S. J., and Parks, J. K., Abnormalities of the electron transport chain in idiopathic Parkinson's disease. Ann. Neurol.26 (1989) 719–723.
Parker, W. D., Filley, C. M., and Parks, J. K., Cytochrome oxidase deficiency in Alzheimer's disease. Neurology 40 (1990) 1302–1303.
Santiago, M., Venero, J. L., Machado, A., and Cano, J., In vivo protection of striatum from MPP+ neurotoxicity by N-methyl-D-aspartate antagonists. Brain Res.586 (1992) 203–207.
Schapira, A. H. V., Mann, V. M., Cooper, J. M., Dexter, D., Daniel, S. E., Jenner, P., Clark, J. B., and Marsden, C. D., Mitochondrial complex I deficiency in Parkinson's disease. J. Neurochem.55 (1990) 2142–2145.
Schneider, J. S., Pope, A., Simpson, K., Taggart, J., Smith, M. G., and DiStefano, L., Recovery from experimental parkinsonism in primates with GM1 ganglioside treatment. Science256 (1992) 843–846.
Schneider, J. S., Stull, N., DiStefano, L., and Iacovitti, L., Increased potency of orally active semisynthetic sphingolipids compared GM1 ganglioside: in vivo and in vitro studies. Soc. Neurosci. Abstr.19 (1993) 1052.
Schöls, L., Reichmann, H., Langkafel, M., Kuhn, W., and Przuntek, H., Mitochondrial disorders in cerebellar ataxias. Mov. Disorders7 (1992) S44.
Schon, E. A., Mitochondrial disorders in muscle, Curr. Opinion Neurol. Neurosurg.6 (1993) 19–26.
Schwarcz, R., Speciale, C., and Turski, W. A., Kynurenines, glia and the pathogenesis of neurodegenerative disorders, in: Parkinsonism and Aging, pp. 97–105. Eds D. B. Calne, G. Comi, D. Crippa, R. Horowski and M. Trabucchi, Raven Press, New York 1990.
Shoffner, J. M., Watts, R. L., Juncos, J. L., Torroni, A., and Wallace, D. C., Mitochondrial oxidative phosphorylation defects in Parkinson's disease. Ann. Neurol.30 (1991) 332–339.
Simonian, N. A., and Hyman, B. T., Cytochrome oxidase is altered in the hippocampal formation in Alzheimer's disease. Neurology43 (1993) A251.
Simpson, J. R., and Isacson, O., Mitochondrial impairment reduces the threshold for in vivo NMDA-mediated neuronal death in the striatum. Exp. Neurol.121 (1993) 57–64.
Smith, R. P., Louis, C. A., Kruszyna, R., and Kruszyna, H., Acute neurotoxicity of sodium azide and nitric oxide. Fund. appl. Toxic.17 (1991) 120–127.
Sonsalla, P. K., Zeevalk, G. D., Manzino, L., Giovanni, A., and Nicklas, W. J., MK-801 fails to protect against the dopaminergic neuropathology produced by systemic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine in mice or intranigral 1-methyl-4-phenylpyridinium in rats. J. Neurochem.58 (1992) 1979–1982.
Spina, M. B., Squinto, S. P., Miller, J., Lindsay, R. M., and Hyman, C., Brain-derived neurotrophic factor protects dopamine neurons against 6-hydroxydopamine and N-methyl-4-phenylpyridinium ion toxicity: involvement of glutathione system. J. Neurochem.59 (1992) 99–106.
Stamler, J. S., Singel, D. J., and Loscalzo, J., Biochemistry of nitric oxide and its redox-activated forms. Science258 (1992) 1898–1902.
Storey, E., Hyman, B. T., Jenkins, B., Brouillet, E., Miller, J. M., Rosen, B. R., Beal, M. F., 1-Methyl-4-phenylpyridinium produces excitotoxic lesions in rat striatum as a result of impairment of oxidative metabolism. J. Neurochem.58 (1992) 1975–1978.
Srivastava, R., Brouillet E., Beal, M. F., Storey, E., and Hyman, B. T., Blockade of 1-methyl-4-phenylpyridinium ion (MPP+) nigral toxicity in the rat by prior decortication or MK-801 treatment: a stereological estimate of neuronal loss. Neurobiol. Aging14 (1993) 295–301.
Tabatabaei, A., Perry, T. L., Hansen, S., and Krieger, C., Partial effect of MK-801 on MPTP-induced reduction of striatal dopamine in mice. Neurosci. Lett141 (1992) 192–194.
Turski, L., Excitatory amino acid antagonist and Parkinson's disease in: Parkinson's Disease — From Basic Research and Early Diagonosis to Long-term Treatment, pp. 97–114. Eds U. K. Rinne, T. Nagatsu and R. Horowski. Medicom, Bussum 1991.
Turski, L., Bressler, K., Rettig, K.-J., Löschmann, P.-A., and Wachtel, H., Protection of substantia nigra from MPP+ neurotoxicity by N-methyl-D-aspartate antagonists. Nature349 (1991) 414–418.
Turski, L., and Melamed, E., Is there a role for excitatory amino acid antagonists? Parkinson's Disease — Controversial Issues in Therapy, pp. 44–53. Eds C. D. Marsden, and W. H. Oertel. Medicom, Bussum 1993.
Turski, L., and Stephens, D. N., Excitatory amino acid antagonists protect mice against MPP+ seizures. Synapse10 (1992) 120–125.
Turski, W. A., Aminooxyacetic acid produces excitotoxic lesions in the rat substantia nigra. Naunyn-Schmiedebergs Arch. Pharmac.344 (1991) R67.
Turski, W. A., Urbańska, E., Sieklucka, M., and Ikonomidou, C., Quinolinate-like neurotoxicity produced by aminooxyacetic acid in rat striatum. 1st International Congress on Amino Acid Research, Vienna — August 7–12, 1989, Book of abstracts.
Turski, W. A., Urbańska, E., Sieklucka, M., and Ikonomidou, C., Quinolinate-like neurotoxicity produced by aminooxyacetic acid in rat striatum. Amino Acids2 (1992) 245–253.
Urbańska, E., Ikonomidou, C., Sieklucka, M., and Turski, W. A., Aminooxyacetic acid produces excitotoxic lesions in the rat striatum. Soc. Neurosci. Abstr.15 (1989) 764.
Urbańska, E., Ikonomidou, C., Sieklucka, M., and Turski, W. A., Aminooxyacetic acid produces excitotoxic lesions in the rat striatum. Synapse9 (1991) 129–135.
Wallace, D. C., Mitochondrial genetics: a paradigm for aging and degenerative diseases. Science256 (1992) 628–632.
Youdim, M. B. H., The biology of toxic events in Parkinson's disease, in: Progress in Research on Neurodegeneration, Vol. I, pp. 67–86. Eds D. B. Calne, R. Horowski, Y. Mizuno, W. H. Poewe, P. Riederer, and M. B. H. Youdim, Birkhäuser, Boston 1993.
Zeevalk, G. D., and Nicklas, W. J., Chemically induced hypoglycemia and anoxia: relationship to glutamate receptor-mediated toxicity in retina. J. Pharmac. exp. Ther.253 (1990) 1285–1292.
Zeevalk, G. D., and Nicklas, W. J., Mechanisms underlying initiation of excitotoxicity associated with metabolic inhibition. J. Pharmac. exp. Ther.257 (1991) 870–878.
Zuddas, A., Oberto, G., Vaglini, F., Fascetti, F., Fornai, F., and Corsini, G. U., MK 801 prevents MPTP-induced parkinsonism in primates. J. Neurochem.59 (1992) 733–739.
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Turski, L., Turski, W.A. Towards an understanding of the role of glutamate in neurodegenerative disorders: energy metabolism and neuropathology. Experientia 49, 1064–1072 (1993). https://doi.org/10.1007/BF01929915
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DOI: https://doi.org/10.1007/BF01929915