Abstract
In order to improve the efficiency of mussel chromosome preparation, a tissue culture procedure has been developed. Mantle and foot explants were grown in tubes in media composed of Eagle's Basal Medium supplemented either with salts or seawater, enriched with egg yolk, adjusted to pH 7.50, and containing penicillin and streptomycin. After 4 days of incubation at 18°C, antibiotics were renewed and after 6–7 days, cultures were ready for harvesting and preparation of microscopical slides. The cultures were a source of actively dividing cells and consistent metaphase spreads were obtained. Evidence from BrdU incorporation suggested that cells could undergo several rounds of replication. The chromsome spreads were good enough for karyotyping and to successfully silver stain the nucleolar organizer regions.
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Cornet, M. A short-term culture method for chromosome preparation from somatic tissues of adult mussel (Mytilus edulis). Experientia 49, 87–90 (1993). https://doi.org/10.1007/BF01928798
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DOI: https://doi.org/10.1007/BF01928798