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Ultrastructural identification of human tonsil T-lymphocytes by peroxidase-conjugated anti-HTLA serum

  • Specialia
  • Haematologica, Immunologica
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Summary

A horse anti-serum rendered specific for human T-lymphocytes was conjugated with peroxidase and used for ultrastructural identification of human tonsil T-lymhocytes. With T- and B-enriched suspensions, virtually all T-lymphocytes were labelled with Po-anti-HTLA, whereas no B-cells were stained with this conjugate. The labelling was found to be irregularly distributed on the plasma membrane of T-cells. Direct identification with specific Po-anti-HTLA conjugate confirm the ultrastructural characteristics of thymus-dependant cells.

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Authors and Affiliations

  1. Laboratoire d' Immunopathologie, Clinique Dermatologique, Hôpital E. Herriot, F-69374, Lyon Cédex 2, (France)

    D. Schmitt & J. Thivolet

  2. Laboratoire d' Immunologie, I.N.S.E.R.M. U 80 et Clinique de Nephrologie, Hôpital E. Herriot, F-69374, Lyon Cédex 2, (France)

    J. Brochier & J. P. Révillard

Authors
  1. D. Schmitt
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  2. J. Brochier
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  3. J. P. Révillard
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  4. J. Thivolet
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Additional information

This work was supported by I.N.S.E.R.M. grants No. 74-5-027-01 and A.T.P. No. 73-16.

Acknowlegments. We are indebted toMartine Blanc who prepared the cell suspensions and toDanielle Germain who did the preparations for electron microscopic examination.

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Schmitt, D., Brochier, J., Révillard, J.P. et al. Ultrastructural identification of human tonsil T-lymphocytes by peroxidase-conjugated anti-HTLA serum. Experientia 32, 1208–1210 (1976). https://doi.org/10.1007/BF01927629

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  • DOI: https://doi.org/10.1007/BF01927629

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