Summary
In the brain of the honeybee (Apis mellifica), it was found that the rRNA content decreases rapidly during the first 2 days of adult life. An increase is observed from days 3 to 5, followed by another decrease to a level inferior to that of day 2 to 3. Except for a rise on the 5th day, 4s RNA remains constant. An unidentified RNA fraction of low molecular weight (D) sharply decreases on day 5. The possibility is discussed that these results reflect changes in RNA metabolism that can be related to the sequences of activity observed in the bees during the first 9 days after hatching.
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Literatur
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Lösung I: 0,1M NaAc pH 5,0; 0,5% Na-Dodecylsulfat 10 μg/ml Polyvinylsulfat; 500 μg/ml Bentonit; 10−4 M MgCl2; 0,1M NaCl.
Phenol: 80% Phenol; 0,1% Hydroxychinolin.
Puffer I: 0,01M NaAc pH 5,1; 10−4 M MgCl2; 20% Saccharose.
Puffer II: 48 ml 1N HCl; 4,95 gTris; 0,46 ml TEMED; ad 50 ml Wasser, pH 5,5.
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Kuhn, O., Kubli, E. & Hauschteck-Jungen, E. Quantitative Bestimmung von RNS-Fraktionen im Gehirn frisch geschlüpfter Bienen (Apis mellifica). Experientia 28, 982–983 (1972). https://doi.org/10.1007/BF01924987
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DOI: https://doi.org/10.1007/BF01924987