Résumé
Utilisant une nouvelle mesure de densité, de haute précision, on étudia l'effet de l'éthanol sur le poids spécifique des membranes des érythrocytes et des synaptosomes. La concentration de l'éthanol qui produit l'anesthésie générale (0.05M) étendit le volume spécifique des membranes à 0.5–0.6%. Aux concentrations de l'éthanol qui produisent l'anesthésie locale (1M) l'extension du volume spécifique fut de 3 et 6%. Ces résultats supportent la théorie de l'extension des membranes par les anesthétiques et suggèrent que l'extension des constituants protéinés des membranes est plus importante que celle des constituants lipides.
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References
P. Seeman, Pharmac. Rev.24, 583 (1972).
E. I. Eger II, C. Lundgren, S. L. Miller andW. C. Stevens, Anesthesiology30, 129 (1969).
K. W. Miller, W. D. M. Paton andE. B. Smith, Nature, Lond.206, 574 (1965).
P. Seeman, S. Roth andH. Schneider, Biochim. biophys. Acta225, 171 (1971).
J. C. Metcalfe, P. Seeman andA. S. V. Burgen, Molec. Pharmac.4, 87 (1968).
S. Roth andP. Seeman, Nature, Lond.231, 284 (1971).
P. Seeman andS. Roth, Biochim. biophys. Acta255, 171 (1972).
H. Eyring Science154, 1609 (1966).
M. J. Lever, K. W. Miller, W. D. M. Paton andE. B. Smith, Nature, Lond.231, 368 (1971).
K. W. Miller, W. D. M. Paton, R. A. Smith andE. B. Smith, Molec. Pharmac.9, 131 (1973).
C. S. Spyropoulos, J. gen. Physiol.40, 849 (1957).
S. H. Roth, R. A. Smith andW. D. M. Paton, submitted for publication (1973).
P. Seeman, M. Chau-Wong andS. Moyyen, Can. J. Physiol. Pharmac.50, 1193 (1972).
O. Kratky, H. Leopold andH. Stabinger, Z. angew. Physiol.27, 273 (1969). technical translation 1583 of Nat. Res. Council (Ottawa, Canada). The instrument used was a precision density meter (Model DMA 02C) made by Anton Paar, K. G., Graz, Austria.
M. J. T. Schneider andA. S. Schneider, J. Membr. Biol.9, 127 (1972).—J. Clifford, B. A. Pethica andE. G. Smith, inMembrane Models and the Formation of Biological Membranes (Eds.L. Bolis andB. A. Pethica; North Holland Publishers, Amsterdam 1968), p. 19.
J. W. Dundee, Anesth. Analg.49, 467 (1970).
A. D. Bangham, A. Rev. Biochem.41, 753 (1972).
S. M. Johnson, K. W. Miller andA. D. Bangham, Biochim. biophys. Acta307, 42 (1973).
J. R. Trudell, W. L. Hubbell andE. N. Cohen, Biochim. biophys. Acta291, 328 (1973).
J. R. Trudell, W. L. Hubbell, E. N. Cohen andJ. J. Kendig Anesthesiology38, 207 (1973).
Appendix: Equation 1 is derived as follows.dms=(mb+mmw+mm)/(Vb+Vmw+Vm), wheremb,mmw andmm are the masses (in g per test-tube) of buffer medium, membrane water, and dry membrane, respectively, and where Vb, Vmw, and Vm are the corresponding volumes (in ml per tube). Dividing all 6 terms by Vs (=volume of suspension in tube), and substituting W formm/Vs,mm/dm for Vm, anddbV formb, one obtainsdms=[(db Vb/Vs)+(mmw/Vs)+W]/[(Vb/Vs)+(Vmw/Vs)+W/dm]. Further substitutingf forVb/Vs, andh formmw/Vs, and rearranging, one arrives at equation 1.
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Acknowledgment. I thank Dr.H. Schneider (National Research Council Ottawa, Canada) for suggesting the use of the density meter, and Ms.M. Chau-Wong, ProfessorK. Yates and Dr.R. McLelland for their help. Supported by Addiction Research Foundation of Ontario, the Ontario Mental Health Foundation, and the Medical Research Council of Canada (grant No. MT-2951).
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Seeman, P. The membrane expansion theory of anesthesia: Direct evidence using ethanol and a high-precision density meter. Experientia 30, 759–760 (1974). https://doi.org/10.1007/BF01924170
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DOI: https://doi.org/10.1007/BF01924170