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A rapid method for determining ATP by the firefly luciferin-luciferase system

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Zusammenfassung

Eine schnelle Methode zur Bestimmung von ATP in picogramm-Mengen wird beschrieben. Die Grundlage für die Methode ist eine Reaktion zwischen ATP und einem Inkubationsansatz, der Luciferin, Mg2+ und partiell gereinigte Luciferase enthält, und ist zur Bestimmung von ATP in biologischem Material von unterschiedlicher Beschaffenheit (Bakterien, Pflanzen-und Tiergewebe) geeignet.

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Authors and Affiliations

  1. United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, 21701, Frederick, Maryland, USA

    H. A. Neufeld, R. D. Towner & Judith Pace

Authors
  1. H. A. Neufeld
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  2. R. D. Towner
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  3. Judith Pace
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Neufeld, H.A., Towner, R.D. & Pace, J. A rapid method for determining ATP by the firefly luciferin-luciferase system. Experientia 31, 391–392 (1975). https://doi.org/10.1007/BF01922604

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  • DOI: https://doi.org/10.1007/BF01922604

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