Summary
Simple light microscopic methods for routine observation and checking cortical granules of vital mammalian eggs will be described. By using interference contrast and dark field techniques, even small cortical granules can easily be examined. These methods are time-saving compared to the usual ones using an electron microscope. They make distribution studies possible without difficulties.
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Nitsch, B., Brück, H.J. & Palm, S. Simple methods for observing cortical granules in living mammalian eggs. Experientia 33, 1252–1253 (1977). https://doi.org/10.1007/BF01922362
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DOI: https://doi.org/10.1007/BF01922362