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Juvenilizing effect of ecdysone mimic RH 5849 in Galleria mellonella larvae

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Abstract

The response of the final instar larvae ofG. mellonella to topical application of the non-steroidal ecdysone mimic, RH 5849, was age-related as well as dose-dependent. In young final instar larvae, moderate doses of RH 5849 induced perfect supernumerary larval moults, but doses equal to and higher than 8.5 μg per larva caused premature formation of larval cuticle and were lethal. Application of RH 5849 significantly increased allatotropic activity of the brain, and also activated synthesis of juvenile hormone (JH) by the corpora cardiaca/corpora allata complex. Simultaneous application of RH 5849 and FMev, a potent inhibitor of JH synthesis, to young final instar larvae lowered the incidence of perfect supernumerary larval moults. We conclude that the effect of RH 5849 on the developmental programme inG. mellonella is mediated by the corpora allata.

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Authors and Affiliations

  1. Department of Invertebrate Physiology, Warsaw University, Zwirki i Wigury 93, 02-089, Warszawa, Poland

    M. Muszyńska-Pytel, P. Mikołajczyk, M. A. Pszczółkowski & B. Cymborowski

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  1. M. Muszyńska-Pytel
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  2. P. Mikołajczyk
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  3. M. A. Pszczółkowski
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  4. B. Cymborowski
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Additional information

We thank Dr K. D. Wing for providing RH 5849 and Dr H. Oberlander for critical comments on an earlier version of the manuscript. Our thanks are also due to Miss Karen Birmingham for correcting the English manuscript. This work was supported by MEN Grant No. G-MEN 51-90.

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Muszyńska-Pytel, M., Mikołajczyk, P., Pszczółkowski, M.A. et al. Juvenilizing effect of ecdysone mimic RH 5849 in Galleria mellonella larvae. Experientia 48, 1013–1017 (1992). https://doi.org/10.1007/BF01919156

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