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Noninvasive, quantitative determination of muscle blood flow in man by a combination of venous-occlusion plethysmography and computed tomography

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Summary

Because of the lack of non-invasive methods for measuring muscle blood flow, quantitative investigations of blood flow in the skeletal muscle of hypertensive subjects are rare. We therefore developed a new method for the determination of muscle blood flow noninvasively and quantitatively by a combination of computed tomography and venous occlusion plethysmography (strain-gauge method).

At two sites on one forearm (p=site of the largest diameter, d=1 cm proximal to the epicondyle lat.) the volumes of tissues [Vt=total volume, VM=muscle volume, Vsk=bone volume, VR=residual volume=Vt−(VM+VSk)] were determined by computed tomograms and total forearm blood flow (Fp and Fd, respectively in ml/100 ml tissue x min) measured by strain-gauge plethysmography. After correcting for the bone volume at the different sites, Fp and Fd were transformed into the absolute influx rates of blood volume (Qp and Qd). From Qp and Qd and the different tissue volumes, the muscle bloof flow (FM in ml/100 ml muscle x min) could be calculated:

$$F_M = 100 \times \frac{{(Q_p * \times V_{R,d} ) - (Q_d * \times V_{R,p} )}}{{(V_{M,p} \times V_{R,d} ) - (V_{M,d} \times V_{R,p} )}}$$

Results thus derived were compared with data from the literature Cooper et al. (17). At rest there was neither a significant difference in Fp (own results: 3.62±1.67, Cooper: 3.25±1.42 ml/100 ml tissue x min, means±S.D.) nor in FM (4.08±2.07 and 3.66±1.57 ml/100 ml muscle x min, respectively), however, Fp and FM were significantly different (p<0.05). In the mean, FM was 13% greater than Fp, range: −40 to +38% (Cooper 15%, range: −17 to +43%). The individual difference could not be predicted by any of the parameters.

Testing the procedure by means of a pharmacological agent (clonidine) with known effects on muscle blood flow (no change) and skin blood flow (decrease) revealed the correct reproduction of this hemodynamic pattern with our method. The usual identification of total with muscle blood flow would have led to false conclusions.

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Author notes

  1. F. Weber

    Present address: Abteilung für Nieren- und Hochdruckkranke im Zentrum für Innere Medizin der Universität (GHS), Hufelandstraße 55, 4300, Essen, FRG

Authors and Affiliations

  1. Abteilung für Nieren- und Hochdruckkranke im Zentrum für Innere Medizin der Universität (GHS), Hufelandstraße 55, 4300, Essen, FRG

    M. Anlauf

  2. Röntgendiagnostisches Zentralinstitut der Universität (GHS), Hufelandstraße 55, 4300, Essen, FRG

    M. Serdarevic

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Dedicated to Prof. Dr. K. D. Bock for his 65th birthday

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Weber, F., Anlauf, M. & Serdarevic, M. Noninvasive, quantitative determination of muscle blood flow in man by a combination of venous-occlusion plethysmography and computed tomography. Basic Res Cardiol 83, 327–341 (1988). https://doi.org/10.1007/BF01907366

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