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Nifedipine limits infarct size for 24 hours in closed chest coronary embolized dogs

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Summary

We studied the ability of nifedipine, a calcium antagonist, to limit infarct size in the closed chest, coronary embolized dog. Immediately after embolization141Ce labelled microspheres were administered into the left ventricle. Myocardium not receiving microspheres was considered to be the region at risk. The nifedipine group (10 dogs) received a bolus (16 μg/kg i.v. over 8 minutes as a loading dose) followed by continuous infusion (1,000 μg/24 hours) 10 min after embolization. The control group (9 dogs) received an equal volume of saline. Twenty-four hours after embolization the dogs were sacrificed, the heart sectioned into 4-mm slices and the slices were stained with tetrazolium to reveal the infarct. The region at risk was determined by autoradiography of the microspheres in the heart slices. Infarct and risk zone volume were determined by planimetric methods. The nifedipine group had a significantly smaller infarct volume to risk zone voluem ratio than the control group (38.7±4.7% vs. 79.5±4.3%, p<0.001). We conclude that nifedipine produces a sustained limitation of infarct size following permanent occlusion of a dog's coronary artery.

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Author notes

  1. J. M. Downey

    Present address: Department of Physiology, College of Medicine, University of South Alabama, 36688, Mobile, Alabama, (USA)

  2. D. M. Yellon

    Present address: Heart Research Unit, The Rayne Institute, St. Thomas' Hospital, SE1, London, UK

Authors and Affiliations

  1. Department of Physiology, College of Medicine, University of South Alabama, 36688, Mobile, Alabama, (USA)

    S. Yoshida, F. R. Friedman & D. E. Chambers

  2. Heart Research Unit, The Rayne Institute, St. Thomas' Hospital, SE1, London, UK

    D. J. Hearse

Authors
  1. S. Yoshida
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  2. J. M. Downey
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  3. F. R. Friedman
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  4. D. E. Chambers
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  5. D. J. Hearse
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  6. D. M. Yellon
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Additional information

Supported by grants from USPHS, Number HL20648 (USA) and Medical Research Council (Great Britain).

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Yoshida, S., Downey, J.M., Friedman, F.R. et al. Nifedipine limits infarct size for 24 hours in closed chest coronary embolized dogs. Basic Res Cardiol 80, 76–87 (1985). https://doi.org/10.1007/BF01906746

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  • DOI: https://doi.org/10.1007/BF01906746

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