Skip to main content
SpringerLink
Log in

Antagonismusin vivo vonBacillus spp. gegenüberRhizoctonia solani undPythium spp.

Antagonismin vivo ofBacillus spp. againstRhizoctonia solani andPythium spp.

  • Published:
Anzeiger für Schädlingskunde, Pflanzenschutz, Umweltschutz Aims and scope Submit manuscript

Abstract

In seed germination tests some isolates ofBacillus spp. showed antagonistic effects againstPythium aphanidermatum andRhizoctonia solani. In thoroughly infected soil, cucumber seeds reached a 40–60% rate of germination in the presence of the best antagonists. Protection to bean seeds was less effective; the germination rate was hardly better than 30%. The efficiency of antagonism reached by several isolates ofBacillus spp. appears to depend on their capacity to compete with other microorganisms or to produce antibiotic substances.

Zusammenfassung

Es konntenBacillus-Isolate gefunden werden, die sich in Keimungsversuchen an Samen als antagonistisch gegenüberPythium aphanidermatum undRhizoctonia solani erwiesen. Die Versuche wurden mit Bohnen- und Gurkensamen durchgeführt. Die wirksamsten Antagonisten konnten in infizierter Erde Auflaufraten bei Gurken von 40–60% erzielen.

Die Wirksamkeit der Antagonisten an Bohnen war niedriger, hier lagen die Keimraten kaum höher als 30%. Offenbar hängt die Wirksamkeit derBacillus-Antagonisten von der Fähigkeit ab Antibiotika zu produzieren und in Konkurrenz zu anderen Mikroorganismen zu treten.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literaturverzeichnis

  • Abo-El-Dahab, M. K.;El-Goorani, M. A., 1964: Antagonistic effect of aBacillus subtilis strain uponErwinia amylovora. Phytopathol.54, 1285–1286.

    Google Scholar 

  • Baker, K. F.;Stavely, J. R.;Thomas, C. A.;Sasser, M.;Macfall, J. S., 1983: Inhibitory effect ofBacillus subtilis onUromyces phaseoli and on development of rust postules on bean leaves. Phythopathol.73, 1148–1152.

    Google Scholar 

  • Baker, C. J.;Stavely, J. R.;Mock, N., 1985: Biocontrol of bean rust byBacillus subtilis under field conditions. Plant Dis.69, 770–772.

    Google Scholar 

  • Baker, C. J.;Stavely, J. R.;Thomas, C. A.;Sasser, H.;McFall, J. S., 1983: Inhibitory effect ofBacillus subtilis onUromyces phaseoli and on development of rust pustules on bean leaves. Phytopathol.73, 1148–1152.

    Google Scholar 

  • Bastos, C. N.;DeFigeiredo, J. M., 1976:In vitro inhibition ofColletotrichum gloesporides, agent of anthracnose of cashew tree, by a substance produced byBacillus subtilis. Fitopatol. Bras.1, 179–182.

    Google Scholar 

  • Braithwaithe, C. W. D., 1978: Inhibition ofSclerotium rolfsii byPseudomonas aeruginosa andBacillus subtilis and its significance in the biological control of southern blight of pigeon pea (Cajanus cajan (L.) Millsp.). Page 197 in: Abstr. Pap. Int. Cong. Plant Pathol, 3rd.

  • Broadbent, P.;Baker, K. F.;Franks, N.;Holland, J., 1977: Effect ofBacillus spp. on increased growth of seedlings in steamed and nontreated soil. Phythopathol.67, 1027–1034.

    Google Scholar 

  • Burr, T. J.;Schroth, M. N.;Suslow, T., 1978: Increased potato yields by treatment of seedpieces with specific strains ofPseudomonas fluorescens andPseudomonas putida. Phytopathol.68, 1377–1383.

    Google Scholar 

  • Christou, T., 1962: Penetration and host-parasite relationships ofRhizoctonia solani in the bean plant. Phytopathol.52, 291–380.

    Google Scholar 

  • Clark, R. L.;Block, C. C., 1984: Belly rot resistence inCucumis sativus. Phytopathol.74, 819. (Abstr).

    Google Scholar 

  • Cook, R. J.;Baker, K. F., 1983: The nature of biological control of plant pathogens. Amer. Phytopathol. Soc., St. Paul, Minnesota.

    Google Scholar 

  • Cubeta, M. A.;Hartman, G. L.;Singlair, J. B., 1985: Interaction betweenBacillus subtilis and fungi associated with soybean seeds. Pl. Dis.69, 506–509.

    Google Scholar 

  • Dambra, V.;Mutter, S., 1986: Parasitism ofTrichoderma harzianum on cystospori ofPolymyxa betae. Phytopathol. Z.115, 61–71.

    Google Scholar 

  • Davidson, F.;Reuzer, H. W., 1978: Persistence ofRhizobium japonicum on the soybean seed coat under controlled temperature and humidity. Appl. Environ. Microbiol.35, 94–96.

    Google Scholar 

  • Ferreira, J. H. S.;Matthee, F. N.;Thomas, A. C., 1991: Biological control ofEutypa lata on grapevine by an antagonistic strain ofBacillus subtilis. Phytopathol.81, 283–287.

    Google Scholar 

  • Pilippi, C.;Bagnoli, G.;Treggi, G.;Picci, G., 1984: Antagonistic effects of soil bacteria onFusarium oxysporum SCHLECHT f. sp.dianthi. Plant and Soil80, 119–125.

    Google Scholar 

  • Fravel, D. R.;Spurr, H. W., 1977: Biocontrol of tobacco brown-spot disease byBacillus cereus subsp.mycoides in a controlled environment. Phytopathol.67, 930–932.

    Google Scholar 

  • Gregory, G. F.;Schreiber, L. R.;Ichada, J., 1984: Microorganisms antagonistic to or producing antibiotic inhibitory toCeratocystis ulmi (Abstr.). Phytopathol.74, 804–805.

    Google Scholar 

  • Houston, B. R., 1945: Culture types and pathogenicity of isolates ofCorticium solani. Phytopathol.35, 371–393.

    Google Scholar 

  • Jones, J. P., 1961: Comparative effects of soil fungicide treatments on soil rot and damping-off of cucumber. Plant Dis.45, 376–379.

    Google Scholar 

  • Katz, E.;Demain, A. C., 1977: The peptide antibiotics ofBacillus: Chemistry, biogenesis and possible functions. Bacteriol. Rev.41, 449–474.

    PubMed  Google Scholar 

  • Krause, C. R.;Ichida, J. M.;Schreiber, L. R.;Gregory, G. F., 1987: Morphological and cytological effects onCeratocystis ulmi of an antifungal antibiotic produced byBacillus subtilis. (Abstr.) Phytopathol.77, 1771.

    Google Scholar 

  • Lewis, J. A.;Papavizas, G. C., 1980: Integrated control ofRhizoctonia fruit rot of cucumber. Phytopathol.70, 85–89.

    Google Scholar 

  • Liu, Z. L.;Sinclair, J. B., 1991: Effects of seed coating byBacillus spp. on suppression ofRhizoctonia damping-off, root and stem rot on soybeans. Biol. Cult. Tests.6, 62.

    Google Scholar 

  • Liu, Z. L.;Sinclair, J. B., 1992: Population dynamics of strain B153-2-2 in the rhizosphere of soybean. Phytopathol.82, 1297–1301.

    Google Scholar 

  • McCarter, S. M.;Littrell, R. H., 1969: Comparative pathogenicity ofPythium aphanidermatum andPythium myriotylum to twelve plant species and intraspecific variation in virulence. Phytopathol.60, 264–268.

    Google Scholar 

  • McKeen, C. D.;Reilly, C. C.;Pusey, P. L., 1986: Production and partial characterisation of antifungal substances antagonistic toMonilia fructicola fromBacillus subtilis. Phytopathol.76, 136–139.

    Google Scholar 

  • Merriman, P. R.;Price, R. D.;Kollmorgen, J. F.;Piggott, T.;Ridge, E. H., 1974: Effect of seedinoculation withBacillus subtilis andStreptomyces griseus on the growth of cereals and carrots. Aust. J. Agric. Res.25, 219–226.

    Google Scholar 

  • Middleton, J. T., 1943: The taxonomy, host range, and geographic distribution of the genusPythium. Mem. Torrey Bot. Club20, 1–171.

    Google Scholar 

  • Mitchell, R.;Alexander, M., 1962: Microbiological processes associated with the use of chitin for biological control. Soil. Sci. Soc. Am. Proc.26, 556–558.

    Google Scholar 

  • Morgan, F. L., 1963: Infection inhibition and germ-tube lysis of three cereal rusts byBacillus pumilus. Phytopathol.53, 1346–1348.

    Google Scholar 

  • Nair, N. G.;Fahy, P. C., 1976: Commerical application of biological control of mushroom bacterial blotch. Aust. J. Agric. Res.27, 415–422.

    Google Scholar 

  • Olsen, C. M.;Baker, K. F., 1968: Selective heat treatment of soil and its effect on inhibition ofRhizoctonia solani byBacillus subtilis. Phytopathol.58, 79–87.

    Google Scholar 

  • Papavizas, G. C., 1985:Trichoderma andGliocladium: Biology, ecology and potential for biocontrol. Ann. Rev. Phytopathol.23, 23–54.

    Google Scholar 

  • Pusey, P. L.;Wilson, C. L., 1984: Postharvest biological control of stone fruit brown rot byBacillus subtilis. Plant Dis.68, 753–756.

    Google Scholar 

  • Pusey, P. L.;Wilson, C. L.;Hotchkiss, M. W.;Franklin, J. D., 1986: Compatibility ofBacillus subtilis for postharvest control of peach brown rot with commerical fruit waxes, Dicloran and cold-storage conditions. Plant Dis.70, 587–590.

    Google Scholar 

  • Schippers, B., 1983: Prospects of biological control of plant pathogens with fluorescentPseudomonas spp. Proc. 10th Int. Congr. Pl. Prot. 767–771.

  • Schmitthenner, A. F., 1972: Effect of light and calcium on germination of oospores ofPythium aphanidermatum. Phytopathol.62, 788.

    Google Scholar 

  • Stanghellini, M. E. 1974: Spore germination, growth and survival ofPythium in soil. Proc. Am. Phytopathol. Soc.1, 211–214.

    Google Scholar 

  • Stanghellini, M. E.;Burr, T. J., 1973: Germinationin vivo ofPythium aphanidermatum oospores and sporangia. Phytopathol.63, 1493–1496.

    Google Scholar 

  • Suslow, T. V., 1980: Growth and yield enhancement of sugar beets by pelleting seed with specificPseudomonas spp. (Abstract). Phytopathol. News12, 40.

    Google Scholar 

  • Suslow, T. V.;Schroth, M. N., 1982: Rhizobacteria of sugar beets: Effects of seed application and root colonization on yield. Phytopathol.72, 199–206.

    Google Scholar 

  • Tahavonen, R., 1982: Preliminary experiments into the use ofStreptomyces spp. isolated from peat in the biological control of soil, and seed-borne diseases in peat culture. J. Sci. Agric. Soc. Finland54, 357–369.

    Google Scholar 

  • Turhan, G., 1981: A new race ofStreptomyces ochraceiscleroticus in the biological control of some soil-borne plant pathogens. Z. Pflanzenk., Pflanzenschutz88, 373–381.

    Google Scholar 

  • Utkehede, R. S.;Rahe, J. E., 1983: Interaction of antagonist and pathogen in biological control of onion white rot. Phytopathol.73, 890–893.

    Google Scholar 

  • Weller, D. M., 1988: Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Ann. Rev. Phytopathol.26, 379–407.

    Google Scholar 

  • Wölk, M.;Sarkar, S., 1993 (a): Antagonismusin vitro von fluoreszierenden Pseudomonaden gegenüberRhizoctonia solani undPythium aphanidermatum. Zentralbl. Microbiol.148 237–245.

    Google Scholar 

  • Wölk, M.; Sarkar, S., 1993 (b): Eine Trockenformulierung für bakterielle Antagonisten zur Bakterisation von Pflanzensamen und Vermehrung der Bakterien in der Rhizosphäre. Zentralbl. Mikrobiol. (im Durck).

  • Wölk, M.;Sarkar, S., 1993 (c): Antagonismusin vitro vonBacillus spp. gegenüberRhizoctonia solani undPythium spp. Anz. Schädlingskde., Pflanzenschutz, Umweltschutz66 (7), 121–125.

    Google Scholar 

  • Wölk, M.; Sarkar, S., 1993 (d): Pathogenität vonPythium aphanidermatum gegenüber Gurken (Cucumis sativus). Zentralbl. Mikrobiol. (im Druck).

Download references

Author information

Author notes

  1. M. Wölk &  S. Sarkar

    Present address: Institut für Phytomedizin, Fachgebiet Virologie und Bakteriologie, Universität Hohenheim, D-70593, Stuttgart-Hohenheim, Deutschland

Authors and Affiliations

    Authors
    1. M. Wölk
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. S. Sarkar
      View author publications

      You can also search for this author in PubMed Google Scholar

    Additional information

    Mit 4 Abbildungen

    Rights and permissions

    Reprints and permissions

    About this article

    Cite this article

    Wölk, M., Sarkar, S. Antagonismusin vivo vonBacillus spp. gegenüberRhizoctonia solani undPythium spp.. Anz. Schadlingskde., Pflanzenschutz, Umweltschutz 67, 1–5 (1994). https://doi.org/10.1007/BF01906560

    Download citation

    • Issue Date:

    • DOI: https://doi.org/10.1007/BF01906560

    Search

    Navigation