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Heat production and oxygen consumption of the isolated rabbit heart: Their relation to mechanical function

Wärmeproduktion und Sauerstoffverbrauch isolierter Kaninchenherzen und ihre Beziehung zur Herzfunktion

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Summary

A method is described for simultaneous determination of the heat production and the oxygen consumption of the isolated, isovolumetrically beating rabbit heart. The perfusion of the heart was performed via the aorta at a constant flow rate with carbogen saturated Tyrode's solution at a temperature of 37.0°C. Heart function was varied by stepwise augmentation of the left intraventricular volume (LVV) by means of a balloon catheter. The following mechanical parameter of heart function were determined: enddiastolic pressure (EDP), peak pressure (PP), developed pressure (DP), max. contraction and relaxation velocity (dP/dt-A and dP/dt-B), enddiastolic tension (Tens-EDP), peak tension (Tens-PP), developed tension (Tens-DP), and circumferential tension (Tens-Cir). DP, dP/dt-A and dP/dt-B showed a maximum response to changes of the LVV at 2.0 ml LVV and 19.3 mm Hg EDP.

Heart production (H) and oxygen consumption (Q) were correlated closely to mechanical function and to each other (r=0.89, n=8). The ratio H/Q was 4.9 cal/ml O2 and remained constant during the experiment.

The myocardial energy consumption was significantly correlated to all contraction parameters with the best fit to DP and Tens-Cir (r=0.934 and 0.933 resp.). On the basis of the calculated mean regression lines, the function-independent and the function-dependent energy consumption were calculated.

Zusammenfassung

In der vorliegenden Arbeit wird eine Methode beschrieben, die, basierend auf demFickschen Prinzip, die gleichzeitige Bestimmung der Wärmeproduktion und des Sauerstoffverbrauchs isolierter, isovolumetrisch kontrahierender Kaninchenherzen erlaubt. Die Herzen wurden volumenkonstant über die Aorta mit carbogengesättigter Tyrodelösung von 37°C perfundiert.

Änderungen der mechanischen Funktion des Myokards wurden durch Variation des linksventrikulären Volumens mit Hilfe eines Ballonkatheters induziert. Folgende Parameter wurden bestimmt: enddiastolischer Druck (EDP), Spitzendruck (PP), Druckamplitude (DP), max. Kontraktions-(dP/dt-A) und Erschlaffungsgeschwindigkeit (dP/dt-B) sowie die Spannungsparameter Spitzenspannung (Tens-PP), Spannungsamplitude (Tens-DP), zirkumferentielle Spannung (Tens-Cir).

Parallel zu der Zunahme der mechanischen Aktivität des Myokards kommt es zu einer signifikanten Zunahme der Wärmeproduktion (H) und des O2-Verbrauches (Q). Q und H sind gut miteinander korreliert (r=0,89). Das Verhältnis H/Q beträgt 4,9 cal/ml und ist weitgehend konstant. Der myokardiale Energieverbrauch war signifikant zu allen Kontraktionsparametern korreliert, am engsten zu DP und Tens-Cir.

Die gleichzeitige Bestimmung des O2-Verbrauches und der Wärmeproduktion des Herzens ermöglicht es, festzustellen, ob [1] Energie über anaerobe Mechanismen gewonnen wurde (H/Q>5,1) oder [2] chemische Energie im Myokard gespeichert wurde (H/Q<4,6).

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Author notes

  1. M. Theisohn

    Present address: Pharmakologisches Institut der Universität Köln, D-5000, Köln 41, Gleueler Straße 24, Federal Republic of Germany

Authors and Affiliations

  1. Institut für Pharmakologie der Medizinischen Hochschule Hannover, Hannover, Deutschland

    M. Theisohn, M. Friedrich, P. Justus, K. Güttler & W. Klaus

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With 7 figures and tables

Supported by the Deutsche Forschungsgemeinschaft.

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Theisohn, M., Friedrich, M., Justus, P. et al. Heat production and oxygen consumption of the isolated rabbit heart: Their relation to mechanical function. Basic Res Cardiol 72, 19–33 (1977). https://doi.org/10.1007/BF01906298

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