Abstract
Cobra snakeNaja naja naja hemoglobin shows four bands on Triton electrophoresis. We present the primary structure of oneα and oneβ chain. The separation of polypeptide chains was achieved by ion exchange chromatography on carboxymethyl cellulose column. The amino acid sequence was established by automatic Edman degradation of the native chains and tryptic and hydrolytic peptides in a gas-phase sequencer. The structural data are compared with those of human and other reptile hemoglobins and reveal not only large variations from human but within reptiles. The amino acid exchanges involve several subunit contacts and heme binding sites. This is the first study on the hemoglobin of a land snake. There are only two amino acid sequences of sea snake hemoglobin (Microcephalophis gracilis gracilis andLiophis miliaris) reported in the literature.
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References
Abbasi, A., and Braunitzer, G. (1991).Biol. Chem. Hoppe-Seyler 372, 473–479.
Abbasi, A., Wells, R. M. G., Brittain, T., and Braunitzer, G. (1988).Biol. Chem. Hoppe-Seyler 369, 755–764.
Alter, B. P., Goff, S. C., Efremov, G. D., Gravely, M. E., and Huisman, T. H. J. (1980).Br. J. Haematol. 44, 527–534.
Bellairs, A. d'A., and Underwood, G. (1951).Biol. Rev. 26, 193–237.
Carroll, R. L. (1969). InBiology of the Reptilia, Vol. 1 (Gans, C., Bellairs, A. d'A., and Parsons, T. S., eds.), Academic Press, New York, pp. 1–44.
Clegg, J. B., Naughton, M. A., and Weatherall, D. J. (1966).J. Mol. Biol. 19, 91–108.
DeSmet, W. H. O. (1978).Acta Zool. Pathol. Antverp. 70, 119–131.
Dessauer, H. C. (1970). InBiology of the Reptilia, Vol. 3 (Gans, C., and Parsons, T. S., eds.), Academic Press, New York, pp. 1–72.
Dessauer, H. C., Fox, W., and Ramirez, J. R. (1957).Arch. Biochem. Biophys. 71, 11–16.
Duguet, M., Chauvet, J.-P., and Acher, R. (1974).FEBS Lett. 47, 333–337.
Edman, P., and Begg, G. (1967).Eur. J. Biochem. 1, 80–91.
Else, P. L., and Hulbert, A. J. (1981).Am. J. Physiol. 240, R3-R9.
Hirs, C. H. W. (1967).Meth. Enzymol. 11, 216–222.
Hirs, C. H. W., Stein, W. H., and Moore, S. (1956).J. Biol. Chem. 221, 151–159.
Howell, B. J., and Rahn, H. (1976). InBiology of the Reptilia, Vol. 5 (Gans, C., and Dawson, W. R., eds.), Academic Press, New York, pp. 335.
Islam, A., Persson, B., Zaidi, Z. H., and Jornvall, H. (1990).J. Protein Chem. 9, 533–541.
Isaacks, R. E., and Harkness, D. R. (1980).Am. Zool. 20, 15–129.
Jauregui-Adell, J. and Marti, J. (1975).Anal. Biochem. 69, 468–473.
Kratzin, H., Yang, C., Krusche, J. U., and Hilschman, N. (1980).Hoppe-Seylers Z. Physiol. Chem. 361, 1591–1598.
Leclercq, F., Schnek, A. G., Braunitzer, G., Stangl, A., and Schrank, B. (1981).Hoppe-Seyler's Z. Physiol. Chem. 362, 1151–1158.
Leclercq, F., Stangl, A., Schrank, B., Vandesasserie, C., Paul, C., Schnek, A. G., Leonis, J., and Braunitzer, G. (1982).Int. J. Peptide Protein Res. 20, 337–350.
Matsuura, M. S. A., Fushitani, K., and Riggs, A. F. (1989).J. Biol. Chem. 264, 5515–5521.
Naqvi, S., Zaidi, Z. H., von Bahr-Lindstrom, H., Carlquist, M., and Jornvall, H. (1983).FEBS Lett. 162, 290–295.
Ogo, S. H., Abe, A. S., and Focesi, A., Jr. (1979).Comp. Biochem. Physiol. 63A, 285–289.
Ogo, S. H., Matsuura, M. S. A., and Focesi, A., Jr. (1984).Comp. Biochem. Physiol. 18A, 587–589.
Pough, F. H. (1969).Comp. Biochem. Physiol. 31, 885–901.
Pough, F. H. (1976).Physiol. Zool. 49, 141–151.
Rodney, M. H., Hunkapiller, M. W., Hood, L. E., and Dreyer, W. J. (1981).J. Biol. Chem. 256, 7990–7997.
Rossi-Fanelli, A., and Antonini, E. (1958).Biochim. Biophys. Acta 30, 608–615.
Rucknagel, K. P., Reischl, E., and Braunitzer, G. (1984).Hoppe-Seyler's Z. Physiol. Chem. 365, 1163–1171.
Rucknagel, K. P., Braunitzer, G., and Wiesner, H. (1988).Biol. Chem. Hoppe-Seyler 369, 1143–1150.
Sullivan, B. (1967).Science 157, 1308–1310.
Sullivan, B. (1974). InChemical Zoology (Florkin, M., and Scheer, B. T., eds.), Academic Press, New York, p. 377.
Wood, S. C., and Lenfant, C. (1976). InBiology of the Reptilia, Vol. 5 (Gans, C., and Dawson, W. R., eds.), Academic Press, New York, p. 225.
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Naqvi, S., Abbasi, A. & Zaidi, Z.H. Primary structure of hemoglobin from cobraNaja naja naja. J Protein Chem 13, 669–679 (1994). https://doi.org/10.1007/BF01886951
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DOI: https://doi.org/10.1007/BF01886951