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Production of β-glucosidase withAspergillus phoenicis QM329 in aqueous two-phase systems

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Biotechnology Techniques

Summary

The production of β-glucosidase withAspergillus phoenicis QM 329 was studied in two different aqueous two-phase systems: polyethylene glycol (PEG) 1550 7.5%/Dextran T2000 9.5% and PEG 8000 4%/polyvinyl alcohol (PVA) 14000 8%. The enzyme concentrations in the top phase of the phase systems were 3.4 IU/ml and 3.2 IU/ml, respectively, compared with 2.0 IU/ml obtained in a regular medium. The total amount of β-glucosidase obtained in the phase systems was 265 IU and 176 IU, respectively, compared with 200 IU in a regular medium.

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References

  • Albertsson, P-Å., (1986).Partition of Cell Particles and Macromolecules, third edition, New York; Wiley & Sons, Inc.

    Google Scholar 

  • Albertsson, P-Å., Cajarville, A., Brooks, D. E., and Tjerneld, F. (1987).Biochim. Biophys. Acta 926, 87–93.

    Google Scholar 

  • Allen, A., Sternberg, D. (1980).Biotechnol. Bioeng. Symp No 10, 189–197.

  • Andersson, E., Johansson, A.-C., and Hahn-Hägerdal, B. (1985).Enzyme Microb. Technol. 7, 333–338.

    Google Scholar 

  • Andersson, E., and Hahn-Hägerdal, B. (1988).Appl. Microbiol. Biotechnol. 29, 329–336.

    Google Scholar 

  • Andersson, E., and Hahn-Hägerdal, B. (1989).Enzyme Microb. Technol. in press.

  • Hägerdal, B., Harris, H., and Pye, E. K. (1979).Biotechnol. Bioeng. 21, 345–355.

    Google Scholar 

  • Kula, M-R., Kroner, K.H., and Hustedt, H. (1982).Adv. Biochem. Eng. 24, 74–118.

    Google Scholar 

  • Persson, I., Tjerneld, F., Hahn-Hägerdal, B. (1984)Enzyme Microb. Technol. 6, 415–418.

    Google Scholar 

  • Persson, I., Tjerneld, F., and Hahn-Hägerdal, B. (1989a).Process Biochem., accepted for publication.

  • Persson, I., Tjerneld, F., and Hahn-Hägerdal, B. (1989b). Submitted for publication.

  • Persson, I., Tjerneld, F., and Hahn-Hägerdal, B. (1989c). Submitted for publication.

  • Ramgren, M., Andersson, E., Hahn-Hägerdal, B. (1988).Appl. Microbiol. Biotechnol. 29, 337–340.

    Google Scholar 

  • Réczey, K., Persson, I., Tjerneld, F., and Hahn-Hägerdal, B. (1989).Biotechnol. Techniques accepted for publication.

  • Sternberg, D., Vijayakumar, P., Reese, E.T. (1977).Can. J. Microbiol. 23, 139–147.

    Google Scholar 

  • Tjerneld, F., Persson, I., Albertsson, P.-Å., Hahn-Hägerdal, B. (1985).Biotechnol. Bioeng. 27, 1036–1043.

    Google Scholar 

  • Vogel, H.J. (1964).The American Naturalist XCVII, 435–446.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biochemistry, Lund University, P O Box 124, S-221 00, Lund, Sweden

    Ingrid Persson & Folke Tjerneld

  2. Department of Applied Microbiology Chemical Center, Lund University, P O Box 124, S-221 00, Lund, Sweden

    Bärbel Hahn-Hägerdal

Authors
  1. Ingrid Persson
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  2. Folke Tjerneld
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  3. Bärbel Hahn-Hägerdal
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Persson, I., Tjerneld, F. & Hahn-Hägerdal, B. Production of β-glucosidase withAspergillus phoenicis QM329 in aqueous two-phase systems. Biotechnol Tech 3, 265–270 (1989). https://doi.org/10.1007/BF01876062

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  • DOI: https://doi.org/10.1007/BF01876062

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