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Selection of organic solvents for in situ extraction of fermentation products fromClostridium thermohydrosulfuricum cultures

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Biotechnology Techniques

Summary

Fifteen organic solvents were examined to determine their biocompatibility for in situ extraction of fermentation products from cultures of the thermophilic anaerobeClostridium thermohydrosul furicum. Five solvents (hexadecane, isooctane, kerosene, oleyl alcohol, Shellsol TD) were found to be non-toxic toClostridium thermohydrosul furicum. Interfacial tensions, phase separation and partition coefficients for ethanol of the biocompatible solvents were compared. With the exception of kerosene, these solvents showed good separation from the aqueous phase. Oleyl alcohol had the highest partition coefficient for ethanol (KD=0.34 at 65°C) and appears to be suitable for extractive ethanol fermentation.

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Authors and Affiliations

  1. Lehrstuhl A für Verfahrenstechnik, Technische Universität München, Arcisstrasse 21, D-8000, München 2, Germany

    Christopher Job & Eckhart Blass

  2. Lehrstuhl für Mikrobiologie, Technische Universität München, Arcisstrasse 21, D-8000, München 2, Germany

    Cosima Schertler & Walter L. Staudenbauer

Authors
  1. Christopher Job
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  2. Cosima Schertler
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  3. Walter L. Staudenbauer
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  4. Eckhart Blass
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Job, C., Schertler, C., Staudenbauer, W.L. et al. Selection of organic solvents for in situ extraction of fermentation products fromClostridium thermohydrosulfuricum cultures. Biotechnol Tech 3, 315–320 (1989). https://doi.org/10.1007/BF01875628

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