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HPLC determination of xylulose formed by enzymatic xylose isomerization in lignocellulose hydrolysates

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Biotechnology Techniques

Summary

In the concentration range appropriate for enzymatic xylose isomerization, xylulose was measured in a lignocellulose hydrolysate using HPLC with two hydrogen loaded ion exchange columns in series. Spent sulphite liquour (SSL) was used as a model for lignocellulose hydrolysates. In buffer the separation took 22 minutes and in SSL the analysis time was 47 minutes due to the presence of ethanol. The enzymatic isomerization of xylose to xylulose was followed directly in SSL, providing a method for the direct determination of xylose isomerase activity in lignocellulose hydrolysates.

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Authors and Affiliations

  1. Applied Microbiology, Chemical Center, University of Lund, P.O. Box 124, S-22100, Lund, Sweden

    Torbjörn Lindén & Bärbel Hahn-Hägerdal

Authors
  1. Torbjörn Lindén
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  2. Bärbel Hahn-Hägerdal
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Lindén, T., Hahn-Hägerdal, B. HPLC determination of xylulose formed by enzymatic xylose isomerization in lignocellulose hydrolysates. Biotechnol Tech 3, 189–192 (1989). https://doi.org/10.1007/BF01875618

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