Abstract
Total, packed cell and, plasma volume estimates were made for the whole body and selected tissues of rainbow trout by the simultaneous injection of radiolabelled trout erythrocyte (51Cr-RBC) and radioiodinated bovine serum albumin (125I-BSA) tracers. Blood volumes were estimated with both markers separately by the tracer-hematocrit method and as the combination of the51Cr-RBC packed cell and125I-BSA plasma volumes. Mean whole body blood volume was significantly less when calculated from the51Cr-RBC tracer data (3.52±0.78 ml/100 g; ±SD) than when calculated with the125I-BSA tracer (5.06±0.86 ml/100 g) or as the sum of the two volumes combined (4.49±0.60 ml/100 g). The whole body hematocrit (28±5%), estimated as the quotient of the51Cr-RBC volume divided by the sum of the125I-BSA and the51Cr-RBC volumes, also was significantly less than the dorsal aortic microhematocrit (36±4%). Estimates of total blood volumes in most tissues were significantly smaller when calculated from the51Cr-RBC data than when calculated by the other two methods. Tissue blood volumes were greatest in highly vascularized and well perfused tissues and least in poorly vascularized tissues. The relative degree of vascularization among tissues generally remained the same regardless of whether the red cell or the plasma tracer was used to calculated blood volume. It is not clear whether the expanded plasma volume is the result of the distribution of erythrocyte-poor blood into the secondary circulation or the result of extravascular exchange of plasma proteins.
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References cited
Altman, P.L. and Dittmar, D.S. 1971. Transcapillary exchange of large molecules: mammals.In Respiration and Circulation. pp. 504–507. Edited by A.L. Altman and D.S. Dittmar. Federation of American Societies for Experimental Biology, Washington, D.C.
Baker, C.H. 1963. Cr51-labelled red cell, I131-fibrinogen, and T-1824 dilution spaces. Am. J. Physiol. 204: 176–180.
Baker, C.H. and Wykoff, H.D. 1961. Time-concentration curves and dilution spaces of T-1824 and I131-labelled proteins in dogs. Am. J. Physiol. 201: 1159–1163.
Barron, M.G., Tarr, B.D. and Hayton, W.L. 1987. Temperature-dependence of cardiac output and regional blood flow in rainbow troutSalmo gairdneri Richardson. J. Fish Biol. 31: 735–744.
Beyenbach, K.W. and Kirschner, L.B. 1975. Kidney and urinary bladder function of the rainbow trout in Mg and Na excretion. Am. J. Physiol. 229: 389–393.
Boland, E.J. and Olson, K.R. 1979. Vascular organization of the catfish gill filament. Cell Tiss. Rev. 198: 487–500.
Burne, R.H. 1929. A system of ‘fine’ vessels associated with lymphatics in the cod (Gadus morrhua). Phil. Trans. Roy. Soc. 217B: 335–366.
Conte, F.P., Wagner, H.H. and Harris, T.O. 1963. Measurement of blood volume in the fish (Salmo gairdneri). Am. J. Physiol. 205: 533–540.
Dewey, W.C. 1959. Vascular-extravascular exchange of I131 plasma proteins in the rat. Am. J. Physiol. 197: 423–431.
Duff, D.W., Fitzgerald, D., Kullman, D., Lipke, D.W., Ward, J. and Olson, K.R. 1987. Blood volume and red cell space in tissues of the rainbow trout.Salmo gairdneri. Comp. Biochem. Physiol. 87A: 393–398.
Everett, N.B., Simmons, B. and Lasher, E.P. 1956. Distribution of blood (Fe59) and plasma (I131) volumes of rats determined by liquid nitrogen freezing. Circ. Res. 4: 419–424.
Gingerich, W.H. 1986. Tissue distribution and elimination of rotenone in rainbow trout. Aquat. Toxicol. 8: 27–40.
Gingerich, W.H., Pityer, R.A. and Rach, J.J. 1987. Estimates of plasma, packed cell and total blood volume in tissues of the rainbow trout (Salmo gairdneri). Comp. Biochem. Physiol. 87A: 251–256.
Hargens, A.R., Millard, R.W. and Johansen, K. 1974. High capillary permeability in fishes. Comp. Biochem. Physiol. 48A: 657–680.
Houston, A.H. and DeWilde, M.A. 1969. Environmental temperature and the body fluid system of the freshwater teleost-III. Hematology and blood volume of thermally acclimated brook trout (Salvelinus fontinalis). Comp. Biochem. Physiol. 28: 837–855.
Huggel, H.J., Lane, H.C. and Dureret, C.G. 1969. Determination de la courbe d'homogenisation et du volume sanguin circulant de la truiteSalmo gairdneri Rich. par la methode de la dilution isotopique de l'131I et du51Cr. J. Physiol. Paris 61: 145–154.
Mosse, P.R.L., 1978. The distribution of capillaries in the somatic musculature of two vertebrate types with particular reference to teleost fish. Cell Tiss. Res. 187: 281–303.
Nikinmaa, M., Soivio, A. and Riolo, E. 1981. Blood volume ofSalmo gairdneri: Influence of ambient temperature. Comp. Biochem. Physiol. 69A: 767–769.
Ohkawa, K., Tsukada, Y., Nunomura, W., Ando, M., Kimura, I., Hara, A., Hibi, N. and Hirai, H. 1987. Main serum protein of rainbow trout (Salmo gairdneri): its biological properties and significance. Comp. Biochem. Physiol. 88B: 497–501.
Olson, K.R. 1981. Morphology and vascular anatomy of the gills of a primitive air-breathing fish, the bowfin (Amia calva). Cell Tiss. Res. 218: 499–517.
Olson, K.R. 1984. Distribution of flow and plasma skimmung in isolated perfused gills of three teleosts. J. Exp. Biol. 232: 319–325.
Olson, K.R. and Kent, B. 1980. The microvasculature of the elasmobranch gill. Cell Tiss. Res. 209: 49–63.
Peters, T. Jr. 1975. Serum albuminIn The Plasma Proteins, Structure, Function, and Genetic Control. pp. 133–181. Edited by F.W. Putnam, Academic Press, New York, London.
Schiffman, R.H. and Fromm, P.O. 1959. Measurements of some physiological parameters in rainbow trout (Salmo gairdneri). Can. J. Zool. 37: 25–32.
Smith, I.S. 1966. Blood volumes of three salmonids. J. Fish. Res. Bd. Can. 23: 1439–1446.
Stevens, E.D. 1968. The effect of exercise on the distribution of blood to various organs in rainbow trout. Comp. Biochem. Physiol. 25: 615–625.
Swan, H. and Nelson, A.W. 1971. Blood volume 1: Critique: Spun vs. Isotope Hematocrit:125IHSA vs.51CrRBC. Ann. Surgery 173: 481–495.
Thorson, T. B. 1961. Partitioning of body water in Osteichthyes: Phylogenetic and ecological implications in aquatic vertebrates. Biol. Bull. 120: 238–254.
Vidt, D.G. and Saperstein, L.A. 1957. Distrubition volumes of T-1824 and Chromium51 labelled red cells immediated following intravenous injection. Circ. Res. 5: 129–132.
Vogel, W.O.P. 1985a. Systemic vascular anastomoses, primary and secondary vessels in fish, and the phylogeny of lymphtics.In Cardiovascular Shunts. pp. 144–159. Edited by K. Johansen and W. W. Burggren. Alfred Benzon Symposium 21, Munksgaard, Copenhagen.
Vogel, W.O.P. 1985b. The caudal heart of fish: not a lymph heart: Acta. Anat. 121: 41–45.
Vogel, W.O.P., Vogel, V. and Scholte, W. 1974. Ultrastructural study of arterio-venous anastomoses in gill filaments ofTilapia mossambica. Cell Tiss. Res. 155: 491–512.
Vogel, W.O.P., Vogel, V. and Pfautsch, M. 1976. Arteriovenous anastomoses in rainbow trout gill filaments. Cell Tiss. Res. 167: 373–385.
Vogel, W.O.P. and Claviez, M. 1981. Vascular specialization in fish, but no evidence for lymphatics. Z. Naturforsch. 36: 490–492.
Zizza, F. and Reeve, E.B. 1958. Erroneous measurement of plasma volume in the rabbit by T-1824. Am. J. Physiol. 194: 522–526.
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Gingerich, W.H., Pityer, R.A. Comparison of whole body and tissue blood volumes in rainbow trout (Salmo gairdneri) with125I bovine serum albumin and51Cr-erythrocyte tracers. Fish Physiol Biochem 6, 39–47 (1989). https://doi.org/10.1007/BF01875602
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DOI: https://doi.org/10.1007/BF01875602