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A low-cost aqueous two phase system for enzyme extraction

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Biotechnology Techniques

Summary

Several low-cost maltodextrins form two liquid phases with polyethylene glycol (PEG) in aqueous solution. We have measured physical properties for three PEG-maltodextrin two-phase systems and demonstrated their bioseparation capabilities by extracting alcohol dehydrogenase from yeast extracts with a PEG bound textile dye.

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Authors and Affiliations

  1. Center for Chemical Engineering, National Bureau of Standards, 325 Broadway, Division 773.10, 80303-3328, Boulder, CO, USA

    David C. Szlag & Kenneth A. Guiliano

Authors
  1. David C. Szlag
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  2. Kenneth A. Guiliano
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Szlag, D.C., Guiliano, K.A. A low-cost aqueous two phase system for enzyme extraction. Biotechnol Tech 2, 277–282 (1988). https://doi.org/10.1007/BF01875542

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  • DOI: https://doi.org/10.1007/BF01875542

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