Abstract
A bacterial strain, CFBP 3388, isolated from Vetch (Vicia sativa, L.) was identified asP. s. pv.syringae on the basis of nutritional and biochemical patterns which were obtained with classical tests and the Biolog™ system. It caused necrotic symptoms typical ofP. s. pv.syringae on bean leaves and pods after artificial inoculation. However, the isolate caused a citrulline-reversible inhibition ofE. coli in phaseolotoxin bioassay. Furthermore, with CFBP 3388 DNA as template a 1900 bp DNA fragment, specific for the phaseolotoxin DNA cluster ofP. s. pv.phaseolicola, was amplified by PCR. This is the first demonstration that an isolate ofP. syringae that is not pv.phaseolicola can produce phaseolotoxin
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Abbreviations
- bp:
-
base pair
- kb:
-
kilobase
- OCT:
-
Ornithine Carbamoyl Transferase
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Tourte, C., Manceau, C. A strain ofPseudomonas syringae which does not belong to pathovarphaseolicola produces phaseolotoxin. Eur J Plant Pathol 101, 483–490 (1995). https://doi.org/10.1007/BF01874471
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DOI: https://doi.org/10.1007/BF01874471