Abstract
A field experiment was set up in 1988 to study the development of rhizomania disease of sugar beet at different inoculum levels of beet necrotic yellow vein virus (BNYVV) in soil. Five, tenfold different, inoculum levels were created by addition of the approximate amounts of 0, 0.5, 5, 50 and 500 kg infested soil per ha (the latter corresponding to 0.01% v/v calculated to the tillage layer). A drip irrigation treatment was applied to study the influence of soil moisture on disease. Susceptible sugar beet, cv. Regina, was grown for three consecutive years.
In the first year, root symptoms were not observed, but BNYVV-infected plants were detected by ELISA in low numbers at all inoculum levels at harvest. After late drilling in 1989, high numbers of infected plants, up to 90–100% in plots with the highest inoculum level, were detected already in June. Root symptoms were also observed from June onwards. In both these years disease incidence increased in time and was significantly influenced by the initial inoculum level. In the third year, the whole field was heavily diseased, and only for the non-irrigated plots incidence differed for different initial inoculum levels. The expression of symptoms by BNYVV-infected plants was influenced by initial inoculum level, thus by the amount and timing of primary infection.
Root weight at harvest was not affected, but sugar content decreased with increasing inoculum level already in 1988, leading to a reduction in sugar yield of 10% at the highest inoculum level. In 1989, both root weight and sugar content decreased progressively with increasing inoculum level, resulting in sugar yield reductions of 11–66% (down to approximately 3000 kg ha−1) for low to high inoculum levels compared to the control. As the control plots became contaminated, all yields were low in 1990, still showing a decrease with increasing inoculum level in the non-irrigated plots, but an overall mean sugar yield of 3323 kg ha−1 for the irrigated ones.
Sodium and α-amino nitrogen content of the root, additional quality parameters determining extractability of sucrose, showed an increase and decrease, respectively, with increasing initial inoculum level already in the first year. The relative differences in contents compared to those from the control were largest for Na content. A significant negative correlation was found between Na (mmol kg−1 root) and sugar content (% of fresh weight); linear for 1988, exponential for 1989 and 1990.
In spring 1989, the infestation of individual plots was assessed using a quantitative bioassay estimating most probable numbers (MPNs) of infective units of BNYVV per 100 g dry soil. The relationship between the MPns determined and root weight, sugar content and sugar yield at harvest could be described by Gompertz curves. The increase in disease incidence with increasing MPN in 1989 was adequately fitted with a logistic equation.
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References
Anonymus (1990) Method for the polarimetric determination of sucrose in sugar beet after clarification with an aluminium sulphate solution. Appendix, pp 88–89 In: Report of the Proceedings of the 20th Session of the International Commission for uniform methods of sugar analysis, Colorado Springs, 3–8 June 1990. Dutton JV (ed), Norwich, 410 pp
Anonymus (1992) Uniforme methode voor gewichtsbepaling, monstername en monsteronderzoek van suikerbieten in Nederland. De Nie LH and Van de Poel PW (eds), published by the Dutch sugar industry, CSM, Amsterdam, 35 pp
Barnett OW (1986) Surveying for plant viruses: design and considerations. In: McLean GD, Garrett RG and Ruesink WG (eds), Plant virus epidemics: monitoring, modelling and predicting outbreaks. Academic Press, Sydney etc, pp 147–166
Brown KF, Messem AB, Dunham RJ and Biscoe PV (1987) Effect of drought on growth and water use of sugar beet. Journal of Agricultural Science, Cambridge 109: 421–435
Bürcky K (1987) Physiologische und pathologische Veränderungen im Stoffwechsel der Zuckerrübe nach Infektion mit dem Aderngelbfleckigkeitsvirus (BNYVV). Proceedings 50th Winter Congress IIRB, Brussels, pp 23–34
Bürcky K and Beiss A (1986) Schädigung der Zuckerrübe durch das Aderngelbfleckigkeits-virus (BNYVV) in Abhängigkeit vom Verseuchungsgrad des Bodens II. Nährstoffgehalt und Saftqualität. Zuckerindustrie 111: 1111–1118
Bürcky K, Büttner G and Winner C (1986) Schädigung der Zuckerrübe durch das Aderngelbfleckigkeitsvirus (BNYVV) in Abhängigkeit vom Verseuchungsgrad des Bodens I. Gewicht, Rübenqualität und Virustiter. Zuckerindustrie 111: 216–223
Büttner G (1992) BSBV — ein neues Rübenvirus. Pflanzenschutz-Praxis 3: 29
Campbell CL (1986) Interpretation and uses of disease progress curves for root diseases. In: Leonard KJ and Fry WE (eds), Plant disease epidemiology: population dynamics and management. Volume 1. Macmillan, New York, pp 38–54
Campbell CL and Madden LV (1990) Introduction to plant disease epidemiology. Wiley, New York, 532 pp
Clark MF and Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34: 421–435
Cochran WG (1953) Sampling techniques. Wiley, New York, Chapman & Hall, London, 330 pp
Davidoff B and Hanks RJ (1989) Sugar beet production as influenced by limited irrigation. Irrigation Science 10: 1–17
Graf A and Isak H (1986) Verfahren zur Bestimmung des Rizomaniabefalls an Zuckerrüben-Stichproben. Zuckerindustrie 111: 138–145
Heijbroek W (1989) The development of rhizomania in two areas of the Netherlands and its effect on sugar-beet growth and quality. Netherlands Journal of Plant Pathology 95: 27–35
Heijbroek W and Van de Bund CF (1982) The influence of some agricultural practices on soil organisms and plant establishment of sugar beet. Netherlands Journal of Plant Pathology 88: 1–17
Henry CM, Jones RCA and Coutts RHA (1986) Occurrence of a soil-borne virus of sugar beet in England. Plant Pathology 35: 585–591
Hillel D (1982) Introduction to soil physics. Academic Press, New York. 364 pp
Hofmeester Y and Tuitert G (1989) Development of rhizomania in an artificially infested field. Mededelingen van de Faculteit Landbouwwetenschappen Rijksuniversiteit Gent 51: 827–834
Kirby EA, Armstrong MJ and Milford GFJ (1987) The absorption and physiological roles of P and K in the sugar beet plant with reference to the function of Na and Mg. Proceedings 50th Winter Congress IIRB (I), Brussels, pp 1–23
Lamers JG (1981) Continuteelt en nauwe rotaties van aardappelen en suikerbieten. PAGV-Lelystad, Publikatie nr 12, 65 pp (Including English summary)
Lesemann D-E, Koenig R, Lindsten K and Henry CM (1989) Serotypes of beet soil-borne furovirus from FRG and Sweden. EPPO Bulletin 19: 539–540
Madden LV and Campbell CL (1990) Nonlinear disease progress curves. In: Kranz J (ed), Epidemics of plant diseases. 2nd ed, Springer-Verlag, Berlin, pp 181–229
Mead R and Curnow RN (1983) Statistical methods in agriculture and experimental biology. Chapman & Hall, London, New York, 335 pp
Müller HJ (1983) Virusbefall und Verhältnis von Inhaltstoffen-auch in Abhängigkeit von Vorfrüchten—im Jahre 1982 in Österreich. Zuckerindustrie 108: 760–763
Müller HJ and Gösswein B (1987) Einfluß von Anbaumethoden insbesondere der Beregnung und der Fruchtfolge auf Rizomania. Proceedings 50th Winter Congress IIRB, Brussels, pp 79–95
Neher DA and Campbell CL (1991) Underestimation of disease progress rates with the logistic, monomolecular, and Gompertz models when maximum disease intensity is less than 100 percent. Phytopathology 82: 811–814
Payne RW, Lane PW, Ainsley AE, Bicknell KE, Digby PGN, Harding SA, Leech PK, Simpson HR, Todd AD, Verrier PJ, White RP, Gower JC, Tunnicliffe Wilson G and Patterson LJ (1988) Genstat 5 Reference Manual. Clarendon Press, Oxford, 749 pp
Pfender WF (1982) Monocyclic and polyclic root diseases: distinguishing between the nature of the disease cycle and the shape of the disease progress curve. Phytopathology 72: 31–32
Pollach G (1984) Versuche zur Verbesserung einer Rizomania-Diagnose auf Basis konventioneller Rübenanalysen. Zuckerindustrie 109: 849–853
Tuitert G (1990) Assessment of the inoculum potential ofPolymyxa betae and beet necrotic yellow vein virus (BNYVV) in soil using the most probable number method. Netherlands Journal of Plant Pathology 96: 331–341
Tuitert G (1993) Horizontal spread of beet necrotic yellow vein virus in soil. Netherlands Journal of Plant Pathology 99: 85–96
Tuitert G and Bollen GJ (1993) Recovery of resting spores ofPolymyxa betae from soil and the influence of duration of the bioassay on the detection level of beet necrotic yellow vein virus in soil. Netherlands Journal of Plant Pathology 99 (Suppl 3): 219–230
Tuitert G and Hofmeester Y (1990) Development of rhizomania at different initial inoculum levels of the soil. Proceedings of the First Symposium of the International Working Group on Plant Viruses with Fungal Vectors, Braunschweig, Germany, August, 21–24, 1990. Schriftenreihe der Deutschen Phytomedizinischen Gesellschaft, Band 1: 73
Tuitert G and Hofmeester Y (1992) Epidemiology of beet necrotic yellow vein virus in sugar beet at different initial inoculum levels in the presence or absence of irrigation: Dynamics of inoculum. Netherlands Journal of Plant Pathology 98: 343–360
Van der Beek MA and Withagen LM (1988) Parameters voor de kwaliteit van suikerbieten. Meststoffen 1: 3–6
Van der Schans DA and Drenth H (1989) De invloed van droogtestress op groei en kwaliteit van suikerbieten. Jaarboek 1987/1988. PAGV-Lelystad, Publikatie nr 43, pp 42–49 (Including English summary)
Van Geijn NJ, Giljam LC and De Nie LH (1983) α-Amino-nitrogen in sugar processing. Proceedings IIRB Symposium ‘Nitrogen and sugar-beet’, Brussels, 16–17 February 1983, pp 13–25
Wieninger L and Rösner G (1983) Zum Verhalten rizomaniageschädigter Rüben in der Saftreinigung. Zuckerindustrie 108: 758–760
Winter SR (1988) Influence of seasonal irrigation amount on sugarbeet yield and quality. Journal of Sugar Beet Research 25: 1–10
Zadoks JC (1978) Methodology of epidemiological research. In: Horsfall JG and Cowling EB (eds) Plant Disease: an advanced treatise. Volume II. Academic Press, New York etc, pp 63–96
Zadoks JC and Schein RD (1979) Epidemiology and Plant Disease Management. Oxford University Press, New York, Oxford, 427 pp
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Tuitert, G., Hofmeester, Y. Epidemiology of beet necrotic yellow vein virus in sugar beet at different initial inoculum levels in the presence or absence of irrigation. Eur J Plant Pathol 100, 19–53 (1994). https://doi.org/10.1007/BF01871964
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DOI: https://doi.org/10.1007/BF01871964