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Thyroid hormone regulation of Na,K-ATPase subunit-mRNA expression in neonatal rat myocardium

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Summary

Regulation of Na,K-ATPase mRNAα isoform and mRNAβ expression by thyroid hormone (T3) in neonatal rat myocardium was examined. In euthyroid neonates between ages of 2 and 5 days, mRNAα1, mRNAα3, and mRNAβ1 abundances were nearly constant while mRNAα2 was undetectable. During the interval between postnatal days 5 and 15, mRNAα3 decreased to negligible levels and mRNAα2 became expressed and increased in abundance to account for ∼20% of the mRNAα pool by the 15th postnatal day. To examine the effect of T3 on this developmental program, neonates were injected with 75 μg T3/100 g body weight or diluent alone on the second and third postnatal days and myocardial Na,K-ATPase subunit-mRNA abundances were determined on the third and fourth postnatal days. Because T3 treatment increased the RNA/DNA ratios of myocardial tissue, the subunit-mRNA abundances were normalized per unit DNA. Following 24 and 48 hr of T3 treatment, the abundances of mRNAα1, mRNAα3, and mRNAβ1 increased, while mRNAα2 continued to remain undetectable during the 2-day interval between the second to fourth postnatal days. It is concluded that T3 augments the abundance of Na,K-ATPase subunit mRNAs that are already being expressed in the neonatal rat myocardium. The results further suggest that T3 does not act as a “molecular switch” in the developmental expression of the mRNAα isoforms in rat myocardium during the first four postnatal days.

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  1. Department of Medicine, College of Physicians and Surgeons, Columbia University, 10032, New York, New York

    Jivan Melikian & Faramarz Ismail-Beigi

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  1. Jivan Melikian
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Melikian, J., Ismail-Beigi, F. Thyroid hormone regulation of Na,K-ATPase subunit-mRNA expression in neonatal rat myocardium. J. Membrain Biol. 119, 171–177 (1991). https://doi.org/10.1007/BF01871416

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  • DOI: https://doi.org/10.1007/BF01871416

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