Summary
The vacuolar equilibrium potential of the lipophilic cation TPMP+ (triphenyl methyl phosphonium) in the giant algaeChara australis andGriffithsia monilis was directly measured. The TPMP+ equilibrium potential was approximately 100mV less negative than the measured vacuolar electrical potential. Thus TPMP+ does not act as a probe of the vacuolar electrical potential and appears to be extruded against an electrochemical gradient. Measurement of the plasmalemma equilibrium potential of TPMP+ showed that extrusion of TPMP+ apparently occurred at both the tonoplast and plasmalemma inChara and at the plasmalemma inGriffithsia. It is concluded that TPMP+ cannot be used as a membrane potential probe inChara orGriffithsia.
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References
Atkins, G.L. 1969. Multicompartment Models in Biological Systems. Methuen and Co., London
Bakker, E.P. 1978. Accumulation of thallous ions (Tl+) as a measure of the electrical potential difference across the cytoplasmic membrane of bacteria.Biochemistry 17:2899–2904
Barts, P.W.J.A., Hoeberichts, J.A., Klaassen, A., Borst-Pauwels, G.W.F.H. 1980. Uptake of the lipophilic cation dibenzyldimethylammonium intoSaccharomyces cerevisiae.Biochim. Biophys. Acta 597:125–136
Beardall, J., Raven, J.A. 1981. Transport of inorganic carbon and the ‘CO2 concentrating mechanism’ inChlorella emersonii (Chlorophyceae).J. Phycol. 17:134–141
Bisson, M.A., Kirst, G.O. 1979a. Osmotic adaption in the marine algaGriffithsia monilis (Rhodophyceae): The role of ions and organic compounds.Aust. J. Plant Physiol. 6:523–538
Bisson, M.A., Kirst, G.O. 1979b Regulation of turgor pressure in marine algae: Ions and low-molecular-weight organic compounds.Aust. J. Plant Physiol. 6:539–556
Bostrom, T.E. 1976. Intercellular Transport of Ions inChara australis. Ph.D. Thesis. The University of Sydney
Briggs, G.E., Hope, A.B., Robertson, R.N. 1961. Electrolytes and Plant Cells. Blackwell Scientific Publ., Oxford
Cheng, F., Haspel, H.C., Vallano, M.L., Osotimehin, B., Sonenberg, M. 1980. Measurement of membrane potentials (Ψ) of erythrocytes and white adipocytes by the accumulation of triphenylmethylphosphonium cation.J. Membrane Biol. 56:191–201
Findlay, G.P., Hope, A.B., Williams, E.J. 1969. Ionic relations of marine algae. I.Griffithsia: membrane electrical properties.Aust. J. Biol. Sci. 22:1163–1178
Findlay, G.P., Hope, A.B., Williams, E.J. 1970. Ionic relations of marine algae. II.Griffithsia: ionic fluxes.Aust. J. Biol. Sci. 23:323–338
Ghazi, A., Schechter, E., Letellier, L., Labedan, B. 1981. Probes of membrane potential inEscherichia coli cells.FEBS Lett. 125:197–200
Grinius, L.L., Jasaitis, A.A., Kadziauskas, Yu.P., Liberman, E.A., Skulachev, V.P., Topali, V.P., Tsofina, L.M., Vladimorova, M.A. 1970. Conversion of biomembrane-produced energy into electric form. I. Submitochondrial particles.Biochim. Biophys. Acta 217:1–12
Harold, P.M., Papineau, D. 1972. Cation transport and electrogenesis byStreptococcus faecalis. I. Membrane potential.J. Membrane Biol. 8:27–44
Hauer, R., Hofer, M. 1978. Evidence for interactions between the energy-dependent transport of sugars and the membrane potential in the yeastRhodotorula gracilis (Rhodosporidium toruloides).J. Membrane Biol. 43:335–349
Hoek, J.B., Nicholls, D.C., Williamson, J.R. 1980. Determination of the mitochondrial protonmotive force in isolated hepatocytes.J. Biol. Chem. 255:1458–1464
Hope, A.B. 1971. Ion Transport and Membranes: A Biophysical Outline. Butterworths, London
Hope, A.B., Walker, N.A. 1975. The Physiology of Giant Algal Cells. Cambridge University Press, Cambridge
Kamo, N., Muratsugu, M., Hongoh, R., Kobatake, Y. 1979. Membrane potential of mitochondria measured with an electrode sensitive to tetraphenyl phosphonium and relationship between proton electrochemical potential and phosphorylation potential in the steady state.J. Membrane Biol. 49:105–121
Komor, E., Tanner, W. 1976. The determination of the membrane potential ofChlorella vulgaris: Evidence for electrogenic sugar transport.Eur. J. Biochem. 70:197–204
Larkum, A.W.D., Weyrauch, S.K. 1977. Photosynthetic action spectra and light harvesting inGriffithsia monilis (Rhodophyta).Photochem. Photobiol. 25:65–72
Liberman, E.A., Topali, V.P., Tsofina, L.M., Jasaitis, A.A., Skulachev, V.P. 1969. Mechanism of coupling of oxidative phosphorylation and the membrane potential of mitochondria.Nature (London) 222:1076–1078
Lichtshtein, D., Kaback, H.R., Blume, A.J. 1979. Use of a lipophilic cation for determination of membrane potential in neuroblastoma-glioma hybrid cell suspensions.Proc. Natl. Acad. Sci. USA 76:650–654
Mewes, H.-W., Rafael, J. 1981. The 2-(dimethylaminostyril)-1-methylpyridinium cation as indicator of the mitochondrial membrane potential.FEBS Lett. 131:7–10
Michel, H., Oesterhelt, D. 1976. Light-induced changes of the pH gradient and the membrane potential inH. halobium.FEBS Lett. 65:175–178
Mitchell, P. 1966. Chemiosmotic Coupling in Oxidative and Photosynthetic Phosphorylation. Glynn Research Ltd., Bodmin, Cornwall, England
Mitchell, P., Moyle, J. 1969. Estimation of membrane potential and pH difference across the cristae membrane of rat liver mitochondria.Eur. J. Biochem. 7:471–484
Raven, J.A. 1976. Transport in algal cells.In: Encyclopedia of Plant Physiology, New Series. U. Lüttge and M.G. Pitman, editors. Vol. 2, Part A: Cells, pp. 129–188. Springer-Verlag, Heidelberg
Reed, R.H., Collins, J.C. 1981. Membrane potential measurements of marine macroalgae:Porphyra purpurea andUlva lactuca.Plant Cell Environ. 4:257–260
Ritchie, R.J., Larkum, A.W.D. 1982. Cation exchange properties of the cell walls ofEnteromorpha intestinalis (L.) link. (Ulvales, Chlorophyta).J. Exp. Bot. 33:125–139
Rubenstein, B. 1978. Use of lipophilic cations to measure the membrane potential of oat leaf protoplasts.Plant Physiol. 62:927–929
Smith, P.T., Walker, N.A. 1981. Studies on the perfused plasmalemma ofChara corallina: I. Current-voltage curves: ATP and potassium dependence.J. Membrane Biol. 60:223–236
Stein, J.R. 1973. Phycological Methods: Culture Methods and Growth measurements. Cambridge University Press, Cambridge
Tanner, W. 1980. Proton sugar cotransport in lower and higher plants.Ber. Dtsch. Bot. Ges. 93:167–176
Walker, N.A. 1982. Membrane transport in charophyte plants: Chemiosmotic but electrically versatile.In: Membranes and Transport: A Critical Review. A. Martinosi, editor. Plenum Press, New York (in press)
Walker, N.A., Pitman, M.G. 1976. Measurement of fluxes across membranes.In: Encyclopedia of Plant Physiology, New Series. U. Lüttge and M.G. Pitman, editors. Vol. 2, Part A: Cells. pp. 93–126. Springer-Verlag, Heidelberg
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Ritchie, R.J. Comparison of a lipophilic cation and microelectrodes to measure membrane potentials of the giant-celled algae,Chara australis (Charophyta) andGriffithsia monilis (Rhodophyta). J. Membrain Biol. 69, 57–63 (1982). https://doi.org/10.1007/BF01871242
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DOI: https://doi.org/10.1007/BF01871242