Summary
Urinary bladders ofBufo marinus were depolarized, by raising the serosal K concentration, to facilitate voltage-clamping of the apical membrane. Passive Na transport across the apical membrane was then studied with near-instantaneous current-voltage curves obtained before and after eliciting a natriferic response with oxytocin. Fitting with the constant-field equation showed that the natriferic effect is accounted for by an increase in the apical Na permeability. It is accompanied by a small increase in cellular Na activity. Furthermore, fluctuation analysis of the amiloride-induced shot-noise component of the short-circuit current indicated that the permeability increase is not due to increased Na translocation through those Na channels which were already conducting prior to hormonal stimulation. Rather, the natriferic effects is found to be based on an increase in the population of transporting channels. It appears that, in response to the hormone, Na channels are rapidly “recruited” from a pool of electrically silent channels.
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References
Aceves, J., Cuthbert, A.W., Edwardson, J.M. 1979. Estimation of the density of sodium entry sites in frog skin epithelium from the uptake of [3H]-benzamil.J. Physiol. (London) 295:477–490
Andersen, B., Ussing, H.H. 1957. Solvent drag on nonelectrolytes during osmotic flow through isolated toad skin and its response to antidiuretic hormone.Acta Physiol. Scand. 39:228–239
Andreoli, T.E., Schafer, J.A. 1976. Mass transport across cell membranes: The effects of antidiuretic hormone on water and solute flows in epithelia.Annu. Rev. Physiol. 38:451–500
Begenisich, T., Stevens, C.F. 1975. How many conductance states do potassium channels have?Biophys. J. 15:843–846
Biber, T.U.L., Cruz, L.J. 1973. Effect of antidiuretic hormone on sodium uptake across outer surface of frog skin.Am. J. Physiol. 225:912–917
Cereijido, M., Herrera, F.C., Flanigan, W.J., Curran, P.F. 1964. The influence of Na-concentration on Na-transport across frog skin.J. Gen. Physiol. 47:879–893
Curran, P.F., Herrera, F.C., Flanigan, W.J. 1963. The effect of Ca and antidiuretic hormone on Na-transport across frog skin. II. Sites and mechanisms of action.J. Gen. Physiol. 46:1011–1027
Cuthbert, A.W. 1976. Amiloride as a probe for sodium entry sites in frog skin epithelium.J. Physiol. (London) 266:28P
Cuthbert, A.W., Painter, E. 1969. Capacitance changes in frog skin caused by theophylline and antidiuretic hormone.Brit. J. Pharmacol. 37:314–324
Cuthbert, A.W., Shum, W.K. 1974. Amiloride and the sodium channel.Naunyn Schmiederbergs Arch. Pharmacol. 281:261–269
Cuthbert, A.W., Shum, W.K. 1975. Effects of vasopressin and aldosterone on amiloride binding in toad bladder epithelial cells.Proc. R. Soc. London B 189:543–575
DiBona, D.R. 1978. Direct visualization of epithelial morphology in the living amphibian urinary bladder.J. Membrane Biol. Special Issue:45–70
Eigler, J., Kelter, J., Renner, E. 1967. Wirkungscharakteristika eines neuen Acylquanidins-Amilorid-HCl (MK 870) an der isolierten Haut von Amphibien.Klin. Wochenschr. 45:737–738
Ekblad, E.B.M., Strum, J.M., Edelman, I.S. 1976. Differential covalent labeling of apical and basal-lateral membranes of the epithelium of the toad bladder.J. Membrane Biol. 26:301–317
Frazier, H.S., Dempsey, E.F., Leaf, A. 1962. Movement of sodium across the mucosal surface of the isolated toad bladder and its modification by vasopressin.J. Gen. Physiol. 45:529–543
Fuchs, W., Hviid Larsen, E., Lindemann, B. 1977. Current-voltage curve of sodium channels and concentration dependence of sodium permeability in frog skin.J. Physiol. (London) 267:137–166
Furhmann, F.A., Ussing, H.H. 1951. A characteristic response of the isolated frog skin potential to neurohypophyseal principles and its relation to the transport of sodium and water.J. Cell. Comp. Physiol. 38:109–130
Gronowicz, G., Masur, S.K., Holtzman, E. 1980. Quantitative analysis of exocytosis and endocytosis in the hydroosmotic response of toad bladder.J. Membrane Biol. 52:221–235
Jörgensen, C.B., Levi, H., Ussing, H.H. 1946. On the influence of neurohypophyseal principles on the sodium metabolism in the axolotl (Amblystoma mexicanuum).Acta Physiol. Scand. 12:350–371
Kachadorian, W.A., Levine, S.D., Wade, J.B., DiScala, V.A., Hays, R.M. 1977. Relationship of aggregated intramembranous particles to water permeability in vasopressin-treated toad urinary bladder.J. Clin. Invest. 59:576–581
Li, J.H.-Y., Palmer, L.G., Edelman, I.S., Lindemann, B. 1979. Effect of ADH on Na-channel parameters in toad urinary bladder.Pfluegers Arch. 382:R13
Lindemann, B., DeFelice, L.J. 1981. On the use of general network functions in the evaluation of noise spectra obtained from epithelia.In: Ion Transport by Epithelia: Recent Advances. S.G. Schultz, editor. Raven Press, New York (in press)
Lindemann, B., Van Driessche, W. 1977. Sodium specific membrane channels of frog skin are pores: Current fluctuations reveal high turnover.Science 195:292–294
Lindemann, B., Van Driessche, W. 1978. The mechanism of Na-uptake through Na-selective channels in the epithelium of frog skin.In: Membrane Transport Processes. J.F. Hoffman, editor. Vol. 1, p. 155. Raven Press, New York
Macknight, A.D.C., DiBona, D.R., Leaf, A. 1980. Sodium transport across toad urinary bladder: A model “tight” epithelium.Physiol. Rev. 60:615–715
Mandel, L.J. 1978. Effects of pH, Ca, ADH, and theophylline on kinetics of Na-entry in frog skin.Am. J. Physiol. 235:C35-C48
Muller, J., Kachadorian, W.A., DiScala, V.A. 1980. Evidence that ADH-stimulated intramembrane particle aggregates are transferred from cytoplasmic to luminal membranes in toad bladder epithelial cells.J. Cell Biol. 85:83–95
Orloff, J., Handler, J. 1962. The similarity of effects of vasopressin, adenosine-3′,5′-phosphate (cyclic AMP) and theophylline on the toad bladder.J. Clin. Invest. 41:702–709
Orloff, J., Handler, J. 1967. The role of adenosine 3′,5′-phosphate in the action of antidiuretic hormone.Am. J. Med. 42:757–768
Palmer, L.G., Edelman, I.S. 1981. Control of apical Na permeability in the toad urinary bladder by aldosterone.Ann. N.Y. Acad. Sci. (in press)
Palmer, L.G., Edelman, I.S., Lindemann, B. 1980. Current-voltage analysis of apical sodium-transport in toad urinary bladders: Effects of inhibitors of transport and metabolism.J. Membrane Biol. 57:59–71
Palmer, L.G., Li, J.H.-Y., Lindemann, G., Edelman, I.S. 1982. Aldosterone control of the density of sodium channels in the toad urinary bladder.J. Membrane Biol. 64:91–102
Roloff, C., Dörge, A., Rick, R., Thurau, K. 1978. Effect of vasopressin on intracellular electrolyte composition of the frog skin.Pfluegers Arch. 377:R40
Stetson, D.L., Lewis, S.A., Wade, J.B. 1981. ADH-induced increase in transepithelial capacitance in toad bladder.Biophys. J. 33:43a
Van Driessche, W., Hegel, U. 1978. Amiloride induced fluctuations of short circuit current through toad urinary bladder. 6th International Biophysics Congress. Kyoto, Japan. p. 215
Van Driessche, W., Lindemann, B. 1978. Low noise amplification of voltage and current fluctuations arising in epithelia.Rev. Sci. Instrum. 49:52–55
Van Driessche, W., Lindemann, B. 1979. Concentration dependence of currents through single sodium-selective pores in frog skin.Nature (London) 282:519–520
Wade, J.B. 1978. Membrane structural specialization of the toad urinary bladder revealed by the freeze-fracture technique. III. Location, structure and vasopressin dependence of intramembrane particle arrays.J. Membrane Biol. Special Issue: 281–296
Warnche, J., Lindemann, B. 1979. A sinewave-burst method to obtain impedance spectra of transporting epithelia during voltage clamp.Pfluegers Arch. 382:R12
Warncke, J., Lindemann, B. 1980. Effect of ADH on the capacitance of apical epithelial membranes.Proc. 28th Int. Congr. Physiol. Sci. (Budapest) pp. 129–133.
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Li, J.H.Y., Palmer, L.G., Edelman, I.S. et al. The role of sodium-channel density in the natriferic response of the toad urinary bladder to an antidiuretic hormone. J. Membrain Biol. 64, 77–89 (1982). https://doi.org/10.1007/BF01870770
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DOI: https://doi.org/10.1007/BF01870770