Summary
A new method is described for orienting the growth of embryonic chick heart cells as thin annuli about nylon monofilament. Analytical measurements of cell water, intracellular potassium, cell volume, and cell surface area incorporate several new techniques and provide the quantitative basis for characterizing the respective cell types in the preparation. The measurements support the hypothesis that tissue culture methodology does not alter the morphological and physiological properties of cardiac muscle cells. The preparations are ideally suited for radiotracer studies since tissue mass can be increased while retaining a relatively short diffusional distance.
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Burrows, R., Lamb, J.F. 1962. Sodium and potassium fluxes in cells cultured from chick embryo heart muscle.J. Physiol. (London) 162:510
Carmeliet, E.E. 1961. Chloride and Potassium Permeability in Cardiac Purkinje Fibres. Editions Arscia S.A., Bruxelles
Carmeliet, E.E., Horres, C.R., Lieberman, M., Vereecke, J.S. 1975. Potassium permeability in the embryonic chick heart: Changes with age, external K and valinomycin.In: Developmental and Physiological Correlates of Cardiac Muscle. M. Lieberman and T. Sano, editors. p. 103. Raven Press, New York
Edelman, G.M., Rutishauser, U., Millette, C.F. 1971. Cell fractionation and arrangement on fibers, beads, and surfaces.Proc. Nat. Acad. Sci. USA 68:2153
Eisenberg, B.R., Kuda, A.M., Peter, J.B. 1974. Stereological analysis of mammalian skeletal muscle. I. Soleus muscle of the adult guinea pig.J. Cell Biol. 60:732
Girardi, A.J., MacMichael, H., Jr., Henle, W. 1956. The use of HeLa cells in suspension for the quantitative study of virus propagation.Virology 2:532
Haas, H.G., Kern, R. 1966. Potassium fluxes in voltage clamped Purkinje fibres.Pfluegers Arch. 291:69
Harrison, R.G. 1914. The reaction of embryonic cells to solid structures.J. Exp. Zool. 17:521
Harsch, M., Green, J.W. 1963. Electrolyte analyses of chick embryonic fluids and heart tissues.J. Cell. Comp. Physiol. 62:319
Horres, C.R. 1975. Potassium tracer kinetics of growth oriented heart cells in tissue culture. Ph. D. Dissertation, Duke University, Durham, N.C.
Horres, C.R., Lieberman, M. 1975. Compartmental analysis of potassium-42 exchange kinetics in embryonic heart.Circulation 52 (Suppl. II): 20 (abstr.)
Horres, C.R., Lieberman, M. 1977. Compartmental analysis of potassium efflux from growth-oriented heart cells.J. Membrane Biol. 34:331
Kaighn, M.E., Ebert, J.D., Stott, P.M. 1966. The susceptibility of differentiating muscle clones to Rous sarcoma virus.Proc. Nat. Acad. Sci. USA 56:133
Karnovsky, M.J. 1965. A formaldehyde-paraformaldehyde fixative of high osmolarity for use in electron microscopy.J. Cell Biol. 27:137a
Keynes, R.D., Lewis, P.R. 1951. The sodium and potassium content of cephalopod nerve fibres.J. Physiol. (London) 114:151
Lieberman, M. 1973. Electrophysiological studies of a synthetic strand of cardiac muscle.Physiologist 16:551
Lieberman, M., Roggeveen, A.E., Purdy, J.E., Johnson, E.A. 1972. Synthetic strands of cardiac muscle: Growth and physiological implication.Science 175:909
Lieberman, M., Sawanobori, T., Kootsey, J.M., Johnson, E.A. 1975. A synthetic strand of cardiac muscle. Its passive electrical properties.J. Gen. Physiol. 65:527
Lieberman, M., Sawanobori, T., Shigeto, N., Johnson, E.A. 1975. Physiologic implications of heart muscle in tissue culture.In: Developmental and Physiological Correlates of Cardiac Muscle. M. Lieberman and T. Sano, editors. p. 139. Raven Press, New York
Manasek, F.J. 1970. Histogenesis of the embryonic myocardiumAm. J. Cardiol. 25:149
McDonald, T.F., DeHaan, R.L. 1973. Ion levels and membrane potential in chick heart tissue and cultured cells.J. Gen. Physiol. 61:89
Okada, T., Nakamori, J., Hirayama, M. 1971. Primary tissue culture method using glass fibre.Hiroshima J. Med. Sci. 20:269
Page, E. 1962. Cat heart muscle in vitro. III. The extracellular space.J. Gen. Physiol. 46:201
Page, E. 1965. Ion movement in heart muscle: Tissue compartments and the experimental definition of driving forces.Ann. N.Y. Acad. Sci. 127:34
Page, E., McCallister, L.P., Power, B. 1971. Stereological measurements of cardiac ultrastructures implicated in excitation contraction coupling.Proc. Nat. Acad. Sci. USA 68:1465
Polinger, I.S. 1970. Separation of cell types in embryonic heart cell cultures.Exp. Cell Res. 63:78
Purdy, J.E., Lieberman, M., Roggeveen, A.E., Kirk, R.G. 1972. Synthetic strands of cardiac muscle. Formation and structure.J. Cell Biol. 55:563
Schmid, H.E., Meschan, I., Watts, F.C., Hosick, T., Muelbaecher, C.A. 1970. Effect of free131I on renal excretion of diatrizoate-131I, an inulin substitute.Am. J. Physiol. 218:903
Sitte, H. 1967. Morphometrische Untersuchungen an Zellen.In: Quantitative Methods in Morphology. E.R. Weibel and H. Elias, editors. p. 167. Springer-Verlag, New York
Sommer, J.R., Johnson, E.A. 1968. Cardiac muscle. A comparative study of Purkinje fibers and ventricular fibers.J. Cell Biol. 36:497
Sommer, J.R., Johnson, E.A. 1969. Cardiac muscle: A comparative ultrastructural study with special reference to frog and chicken hearts.Z. Zellforsch. 98:437
Waymouth, C. 1974. To disaggregate or not to disaggreegate. Injury and cell disaggregation, transient or permanent?In Vitro 10:97
Weibel, E.R. 1972. A stereological method for estimating volume and surface of sarcoplasmic reticulum.J. Microscop. 95:229
Weibel, E.R. 1973. Stereological techniques for electron microscopic morphometry.In: Principles and Techniques of Electron Microscopy Biological Applications. M.A. Hayat, editor. Vol. 3, p. 239. Van Nostrand Rheinhold Company, New York
Yaffe, D. 1968. Retention of differentiation potentialities during prolonged cultivation of myogenic cells.Proc. Nat. Acad. Sci. USA 61:477
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Horres, C.R., Lieberman, M. & Purdy, J.E. Growth orientation of heart cells on nylon monofilament. J. Membrain Biol. 34, 313–329 (1977). https://doi.org/10.1007/BF01870306
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DOI: https://doi.org/10.1007/BF01870306