Summary
The active hexose transport system ofChlorella vulgaris is obligatorily coupled to metabolic energy. No facilitated diffusion component, as in the β-galactoside transport ofEscherichia coli, for example, is observed withChlorella. In the presence of nystatin, however, facilitated diffusion of sugar analogues occurs. Thus, only under this condition can the classical overshoot experiment be successfully carried out. The net efflux of sugars induced by nystatin does not take place through holes. It can be explained by the assumption that the mobility of the unloaded carrier is less restricted in the presence of nystatin. Nystatin also changes theK m for influx, whereas theK m for efflux is not significantly affected. Possible roles of sterols in this eucaryotic sugar transport system are discussed.
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Komor, B., Komor, E. & Tanner, W. Transformation of a strictly coupled active transport system into a facilitated diffusion system by nystatin. J. Membrain Biol. 17, 231–238 (1974). https://doi.org/10.1007/BF01870184
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DOI: https://doi.org/10.1007/BF01870184