Summary
The permeation of various cations and biogenic amines across artificial lipid membranes (bilayer membranes) was investigated by means of electrical conductivity measurements and fluorescence spectroscopy. Their permeability properties were modified by doping them with five different carboxylic ionophores. The induced permeability changes were correlated with some biological activities of the ionophores.
Four out of five ionophores increased the permeability of doped membranes for Li+, Na+, K+, Mg2+ and Ca2+. Two of them showed a preference for K+ whereas one (X-537A) increased the membrane permeability for K+ as well as for Ca2+. It was also found that the ionophores increased the permeability for serotonin, dopamine, norepinephrine and epinephrine. No direct coupling was found between the facilitated ion permeation and the permeation of biogenic amines induced by the ionophores. The measurements can be qualitatively explained by assuming that the permeation of biogenic amines is competitively inhibited by cations. It appears that one biogenic amine molecule forms a complex with one ionophore molecule, the complex acting as a carrier for biogenic amines. All ionophores investigated increased the bilayer permeability considerably for some biogenic amines. (A preference up to 420∶1 for serotonin over epinephrine was measured for one specific ionophore.)
There was no correlation between thein vitro antibacterial activity (against bacillus E and bacillus TA) of the ionophores and their potency to change the ion permeability of doped membranes. The correlation found between the ionophore-induced permeation of biogenic amines through membranes and their antibacterial activity is probably without biological meaning. However, a rather good correlation was found between cardiac sympathetic effects of the ionophores and their ability to facilitate permeation of norepinephrine through artificial membranes.
Similar content being viewed by others
References
Bean, R. C., Shepherd, W. C., Chan, H. 1968. Permeability of lipid bilayer membranes to organic solutes.J. Gen. Physiol. 52:495
Berger, J., Rachlin, A. I., Scott, W. E., Sternbach, L. H., Goldberg, M. W. 1951. The isolation of three new crystalline antibiotics from streptomyces.J. Amer. Chem. Soc. 73:5295
Haeusler, G., Haefely, W., Thoenen, H. 1969. Chemical sympathectomy in the cat with 6-hydroxydopamine.J. Pharmacol. 170:50
Harned, R. L., Hidy, P. H., Corum, C. J., Jones, K. L. 1951. Nigericin, a new crystalline antibiotic from an unidentified streptomyces.Antibiot. Chemother. 1:594
Henderson, B. J. F., McGivan, J. D., Chappell, J. B. 1969. The action of certain antibiotics on mitochondrial, erythrocyte and artificial phospholipid membranes.Biochem. J. 111:521
Johnson, S. M., Herrin, J., Liu, S. J., Paul, I. C. 1970. The crystal and molecular structure of the barium salt of an antibiotic containing a high proportion of oxygen.J. Amer. Chem. Soc. 92:4428
Langendorff, O. 1895. Untersuchungen am überlebenden Säugetierherzen.Arch. Ges. Physiol. 61:291
Lardy, H. A., Graven, S. N., Estrada-O, S. 1967. Specific induction and inhibition of cation and anion transport in mitochondria.Fed. Proc. 26:1355
Läuger, P. 1972. Carrier-mediated ion transport.Science 178:24
Levy, J. V., Cohen, J. A., Inesi, G. 1973. Contractile effects of a calcium ionophore.Nature 242:461
Moore, C., Pressman, B. C. 1964. Mechanism for action of valinomycin on mitochondria.Biochem. Biophys. Res. Commun. 15:562
Mueller, P., Rudin, D. O. 1969. Translocators in bimolecular lipid membranes: Their role in dissipative and conservative bioenergy transductions.Curr. Top. Bioenerget. 3:157
Mueller, P., Rudin, D. O., Tien, H. T., Wescott, W. C. 1964. Formation and properties of bimolecular lipid membranes.In: Recent Progress in Surface Science. J. F. Danielli, K. G. A. Pankhurst and A. C. Riddiford, editors. Vol. 1, p. 379. Academic Press, New York
Pressman, B. C. 1968. Ionophorous antibiotics as models for biological transport.Fed. Proc. 27:1283
Pressman, B. C. 1970. Antibiotic models for carrier-mediated transport through membranes.Antimicrob. Chemother. 1969:28
Pressman, B. C. 1972. Carboxylic ionophores as model carriers for divalent ions.In: The Role of Membranes in Metabolic Regulation. M. A. Mehlman and R. W. Hanson, editors. p. 149. Academic Press, New York
Pressman, B. C. 1973. Properties of ionophores with broad range cation selectivity.Fed. Proc. 32:1698
Pressman, B. C., de Guzman, N. T. 1974. New ionophores for old organells.Ann. N.Y. Acad. Sci. 227:380
Pressman, B. C., Harris, E. J., Jagger, W. S., Johnson, J. H. 1967. Antibiotic mediated transport of alkali ions across lipid barriers.Proc. Nat. Acad. Sci. 58:1949
Reed, P. W., Lardy, H. A. 1972. A divalent cation ionophore.J. Biol. Chem. 247:6970
Scarpa, A., Inesi, G. 1972. Ionophore mediated equilibration of calcium in gradients in fragmented sarcoplasmatic reticulum.FEBS Lett. 22:273
Schadt, M. 1973. Photoresponse of bimolecular lipid membranes pigmented with retinal and vitamin A acid.Biochim. Biophys. Acta 223:351
Stein, W. D. 1967. The movement of molecules across cell membranes.In: Theoretical and Experimental Biology. W. D. Stein, editor. Vol. 6, p. 36. Academic Press, New York
Westley, J. W., Oliveto, E. P., Berger, J., Evans, R. H., Glass, R., Stempel, A., Toome, V., Williams, T. 1973. Chemical transformations of antibiotic X-537 A and their effect on antibacterial activity.J. Med. Chem. 16:397
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schadt, M., Haeusler, G. Permeability of lipid bilayer membranes to biogenic amines and cations: Changes induced by ionophores and correlations with biological activities. J. Membrain Biol. 18, 277–294 (1974). https://doi.org/10.1007/BF01870117
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01870117