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Dimensions of polar pathways through rabbit gallbladder epithelium

The effect of phloretin on nonelectrolyte permeability

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Summary

The permeability of rabbit gallbladder to hydrophilic nonelectrolytes, with molecular weights from 20 to 60,000, has been studied. Restriction in the diffusion of the small electrolytes is very significant up to glycerol, which suggests permeation through aqueous pores with equivalent radii of 4 Å. An extracellular pathway is responsible for the permeation of the larger solutes. This extracellular pathway shows no restriction in diffusion of molecules up to the size of inulin. Dextran (15,000 to 17,000 mol wt) is significantly restricted. Albumin permeability is <10−8 cm sec−1. These observations can be equated with equivalent, pore radii of ≈40 Å for the shunt pathway.

Increasing osmolarities of the incubation medium cause decreased cell-membrane permeability and increased shunt permeability. 0.5mm phloretin induces a 60% reduction in urea permeability and a 168% increase in antipyrine permeability. No effect on the osmotic water permeability or on the shunt permeability is observed in the presence of phloretin. The apparent activation energy of urea permeation changes from values consistent with diffusion in bulk water, to values consistent with diffusion through hydrocarbon regions. This suggests that the polar route for urea permeation is blocked by phloretin.

The contribution of the shunt pathway to osmotic flow induced by sucrose or NaCl gradients is smaller than 16% according to Poiseuille's flow calculations. Tetraethylammoniumchloride and albumin have been shown to be osmotically more effective than sucrose, suggesting a greater shunt contribution to the total water flow.

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  1. Department of Physiology, University of Nijmegen, Nijmegen, The Netherlands

    C. H. van Os, M. D. de Jong & J. F. G. Slegers

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van Os, C.H., de Jong, M.D. & Slegers, J.F.G. Dimensions of polar pathways through rabbit gallbladder epithelium. J. Membrain Biol. 15, 363–382 (1974). https://doi.org/10.1007/BF01870095

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