Summary
Studies were performed to characterize the binding1 of bile acids to intestinal brush border membranes. Total14C-taurodeoxycholate binding was: 1) similar for brush borders prepared from jejunum and ileum, 2) linear with respect to monomer concentration, 3) uninhibited by a structural analog, and 4) not depressed by boiling or trypsin. A linear relationship existed between binding and the number of hydrogen bonds formed by a bile acid and the slope of the line corresponded to σΔF of 300 cal/mol. The binding of bile acids to the 105,000×g supernatant fraction of sonicated brush borders was similar to the binding of phospholipid liposomes using gel chromatography. These data suggest that: 1) the kinetics and characteristics of binding of bile acid to ileal brush borders do not reflect the kinetics and characteristics of active ileal transport previously obtained in whole tissue preparations, but instead reflect the kinetics and characteristics of passive jejunal transport; 2) a determinant of binding is hydrogen bonding with water; 3) isolated intact brush borders are relatively polar membranes; and 4) binding to solubilized brush borders may represent partitioning between the aqueous phase and membrane lipid.
Similar content being viewed by others
References
Accatino, L., Simon, F.R. 1976. Identification and characterization of a bile acid receptor in isolated liver surface membranes.J. Clin. Invest. 57:496
Dahlqvist, A. 1964. Method for assay of intestinal disaccharidases.Anal. Biochem. 7:18
Diamond, J.M., Wright, E.M. 1969. Biological membranes: The physical basis of ion and nonelectrolyte selectivity.Annu. Rev. Physiol. 31:581
Dietschy, J.M., Salomon, H.S., Siperstein, M.D. 1966. Bile acid metabolism. I. Studies on the mechanisms of intestinal transport.J. Clin. Invest. 45:832
Eichholz, A., Howell, K.E. 1972. Progress in gastroenterology. Binding studies as an approach to the study of intestinal transport.Gastroenterology 62:647
Fleischer, S., Fleischer, B. 1967. Removal and binding of polar lipids in mitochondria and other membrane systems.In: Methods in Enzymology. R.N. Estabrook and M.E. Pullman, editors. Vol. 10, p. 406. Academic Press Inc., New York
Forstner, G.G., Sabesin, S.M., Isselbacher, K.J. 1968. Rat intestinal microvillous membranes. Purification and biochemical characterization.Biochem. J. 106:381
Fujita, M., Ohta, H., Kawai, I., Matsui, H., Nakao, M. 1972. Differential isolation microvillous and basolateral plasma membranes from intestinal mucosa: Mutually exclusive distribution of digestive enzymes and ouabain-sensitive ATPase.Biochim. Biophys. Acta 274:336
Holt, P.R. 1964. Intestinal absorption of bile salts in the rat.Am. J. Physiol. 207:1
Houghton, S.E., McCarthy, C.F. 1973. The isolation, partial characterization, and subfractionation of human intestinal brush borders.GUT 14:529
Hummel, J.P., Dreyer, W.J. 1962. Measurement of protein-binding penomena by gel filtration.Biochim. Biophys. Acta 63:530
Katz, Y., Diamond, J.M. 1974. A method for measuring nonelectrolyte partition coefficients between liposomes and water.J. Membrane Biol. 17:69
Kawai, K., Fujita, M., Nakao, M. 1974. Lipid components of two different regions of an intestinal epithelial cell membrane of mouse.Biochim. Biophys. Acta 389:222
Krag, E., Phillips, S.F. 1974. Active and passive bile acid absorption in man. Perfusion studies of the ileum and jejunum.J. Clin. Invest. 53:1686
Lack, L., Weiner, I.M. 1966. Intestinal bile salt transport: Structure-activity relationships and other properties.Am. J. Physiol. 210:1142
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265
Makino, S., Reynolds, J.A., Tanford, C. 1973. The binding of deoxycholate and Triton X-100 to proteins.J. Biol. Chem. 248:4926
Metcalfe, J.C., Seeman, P., Burgen, A.S.V. 1968. The proton relaxation of benzyl alcohol in erythrocyte membranes.Mol. Pharmacol. 4:87
Pardee, A.B. 1968. Membrane transport proteins.Science 162:632
Parkinson, D.K., Ebel, H., DiBona, D.R., Sharp, G.W.G. 1972. Localization of the action of cholera toxin on adenyl cyclase in mucosal epithelial cells of rabbit intestine.J. Clin. Invest. 51:2293
Quigley, J.P., Gotterer, G.S. 1969. Distribution of (Na+−K+)-stimulated ATPase activity in rat intestinal mucosa.Biochim. Biophys. Acta 173:456
Robinson, J.R. 1949. Some effects of glucose and calcium upon the metabolism of kidney slices from adult and newborn rats.Biochem. J. 45:68
Rouser, G., Fleischer, S. 1967. I. Determination of phospholipids by phosphorus analysis. J. Isolation of phospholipids by TLC and Sephadex column chromatography.In: Methods in Enzymology. R.N. Estabrook and M.E. Pullman, editors. Vol. 10, p. 404. Academic Press Inc., New York
Schiff, E.R., Small, N.C., Dietschy, J.M. 1972. Characterization of the kinetics of the passive and active transport mechanisms for bile acid absorption in the small intestine and colon of the rat.J. Clin. Invest. 210:489
Seeman, P. 1969. Temperature dependence of erythrocyte membrane expansion by alcohol anesthetics. Possible support for the partition theory of anesthetics.Biochim. Biophys. Acta 183:520
Small, D.M. 1971. The physical chemistry of cholanic acids.In: The Bile Acids. P.P. Nair and S. Kritchevsky, editors. Vol. 1, p. 249. Plenum Press, New York
Smigel, M., Frölich, J.C., Fleischer, S. 1974. Characterization of prostaglandin E receptor on membranes and its use in the assay of prostaglandin E1.In: Methods in Enzymology. S. Fleischer and L. Packer, editors. Vol. 32, p. 109. Academic Press Inc., New York
Stein, W.D. 1967. The Movement of Molecules Across Cell Membranes. p. 65. Academic Press Inc., New York
Taussky, H.H., Shorr, E. 1953. A microcolorimetric method for the determination of inorganic phosphorus.J. Biol. Chem. 202:675
Westergaard, H., Dietschy, J.M. 1974. Delineation of the dimensions and permeability characteristics of the two major diffusion barriers to passive mucosal uptake in the rabbit intestine.J. Clin. Invest. 54:718
Wilson, F.A., Dietschy, J.M. 1972. Characterization of bile acid absorption across the unstirred water layer and brush border of the rat jejunum.J. Clin. Invest. 51:3015
Wilson, F.A., Treanor, L.L. 1975. Characterization of the passive and active transport mechanisms for bile acid uptake into rat isolated intestinal epithelial cells.Biochim. Biophys. Acta 406:280
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wilson, F.A., Treanor, L.L. Characterization of bile acid binding to rat intestinal brush border membranes. J. Membrain Biol. 33, 213–230 (1977). https://doi.org/10.1007/BF01869517
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01869517