Summary
Addition of noradrenaline (4×10−5 m) to the inner bathing fluid in the skin of the frogRana esculenta results in increased unidirectional fluxes of urea, thiourea, N-methyl-thiourea, N-N′-dimethylthiourea and mannitol. Fluxes towards the external medium (Φ 0) undergo a much greater increase than those moving in the opposite direction (Φ i ). The effect of noradrenaline on (Φ 0) is higher for urea and thiourea than mannitol, while its effect on (Φ 0) thiourea derivatives is related to lipid solubility. This phenomenon does not occur for (Φ i ) of the same molecules.
FCCP (10−6 m) pretreatment strongly inhibits the noradrenaline effect on (Φ 0). In skin pretreated whith colchicine (2×10−5 m) both urea fluxes are increased to the same extent by noradrenaline. Noradrenaline is concluded to exert two separate effects: (1) a change in permeability in both directions; (2) a secretion of nonelectrolytes towards the external fluid. Such secretion is most probably associated with the hormone-induced secretion of fluid and electrolytes, perhaps mediated by an exocytotic mechanism.
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Amsterdam, A., Ohad, I., Schramm, M. 1969. Dynamic changes in the ultrastructure of the acinar cell of the rat parotid gland during the secretory cycle.J. Cell. Biol. 41:753
Bänerlein, E., Keil, R. 1976. Enzyme secretion mediated by the epinephrine β-receptor in rat parotid slices.FEBS Lett. 61:68
Batzri, S., Selinger, Z. 1973. Lipophilic thiourea and thiouracil as inhibitors of oxidative phosphorylation.J. Biol. Chem. 248:356
Campbell, J.P., Huf, E.G. 1967. Electrolytes in frog skin secretion.Comp. Biochem. Physiol. 23:213
Field, M., Sheerin, H., Henderson, A., Smith, P. 1975. Catecholamine effect on cyclic AMP levels and ion secretion in rabbit ileal mucosa.Am. J. Physiol. 229:86
House, C.R. 1970. The effect of noradrenaline on the toad skin potential.J. Physiol. (London) 209:513
Inouè, S., Sato, H. 1967. Cell mobility by labile association of molecules. The nature of mitotic spindle fibers and their role in chromosome movement.J. Gen. Physiol. 50 (suppl.):259
Rasmussen, H. 1970. Cell communication, calcium ion and cyclic adenosine monophosphate.Science 170:404
Svelto, M., Storelli, C., Lippe, C. 1973. Transport of some nonelectrolytes through amphibian skins.Comp. Biochem. Physiol. 46:221
Taylor, A., Mamelak, M., Reaven, E., Maffly, R. 1973. Vasopressin: Possible role of microtubules and microfilaments in its action.Science 181:347
Ussing, H.H., Erlij, D., Lassen, U. 1974. Transport pathways in biological membranes.Ann. Rev. Physiol. 36:17
Vredgenhil, A.P., Roukema, P.A. 1975. Comparison of the secretory processes in the parotid and sublingual glands of the mouse. Regulation of the secretory processes.Biochim. Biophys. Acta 163:494
Wilson, L. 1970. Properties of colchicine binding protein from chick embryo brain. Interaction with vinco alkaloids and podophyllotoxine.Biochemistry 9:4999
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Svelto, M., Perrini, M.C.R. & Lippe, C. Noradrenaline induced secretion of nonelectrolytes through frog skin. J. Membrain Biol. 36, 1–11 (1977). https://doi.org/10.1007/BF01868140
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DOI: https://doi.org/10.1007/BF01868140