Summary
Plasma 11-hydroxycorticosteroid levels were measured fluorometrically during anaphylactic shock of guinea pigs.
Whereas in the acute anaphylactic reaction elicited by i.v. injection of antigen, no increase was observed, a highly significant rise of plasma corticosteroids was found in the so-called protracted shock.
Pretreatment with theα-receptor blocking agent, phentolamine, reduced the plasma corticosteroid levels in the protracted shock significantly. Antihistaminics, and propranolol, had no inhibitory effect.
Zusammenfassung
Die 11-Hydroxycorticosteroid-Konzentrationen im Plasma anaphylaktischer Meerschweinchen wurden fluorometrisch gemessen.
Im akuten anaphylaktischen Schock, der durch i.v. Gabe von Antigen ausgelöst wurde, waren die Plasmaspiegel nicht erhöht, während im sog. protrahierten Schock ein hochsignifikanter Anstieg gemessen wurde.
Vorbehandlung mit demα-Receptorenblocker Phentolamin verminderte die Corticosteroidspiegel im protrahierten Schock signifikant, während Propranolol sowie Antihistaminica keinen Hemmeffekt hatten.
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References
Bernauer, W., Filipowski, P.: On the significance of anaphylactic catecholamine release in guinea pigs. Naunyn-Schmiedebergs Arch. Pharmacol.276, 35–47 (1973).
Bernauer, W., Gossow, U., Hahn, F.: The role of catecholamines in mepyramine resistance of anaphylactic bronchospasm in vitro. Int. Arch. Allergy37, 311–324 (1970).
Bernauer, W., Hagedorn, M., Filipowski, P.: Catecholamine release during anaphylactic shock in guinea pigs. Naunyn-Schmiedebergs Arch. Pharmak.270, 326–334 (1971).
Bhargava, K. P., Bhargava, R., Gupta, M. B.: Central adrenoceptors concerned in the release of adrenocorticotrophic hormone. Brit. J. Pharmacol.45, 682–683 (1972).
Bullock, L. P., New, M. I.: Testosterone and cortisol concentration in spermatic, adrenal and systemic venous blood in adult male guinea pigs. Endocrinology88, 523–526 (1971).
Collier, H. O. J., James, G. W. L.: Humoral factors affecting pulmonary inflation during acute anaphylaxis in the guinea-pig in vivo. Brit. J. Pharmacol.30, 283–301 (1967).
Fajer, A. B., Vogt, M.: Adrenocortical secretion in the guinea-pig. J. Physiol. (Lond.)169, 373–385 (1963).
Giertz, H., Hahn, F., Jurna, J., Schmutzler, W.: Vergleichende Untersuchungen über den anaphylaktischen Schock und den Anaphylatoxinschock am intakten Meerschweinchen. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak.242, 65–75 (1961).
Graef, V., Staudinger, Hj.: Untersuchungen zur Spezifität der fluorometrischen Bestimmung der 11-Hydroxycorticosteroide im Plasma. Z. klin. Chem. klin. Biochem.8, 368–373 (1970).
Green, H. F.: The antagonism of histamine and the anaphylactic response by phenylpyridylallylamines. Brit. J. Pharmacol.8, 171–176 (1953).
Guillemin, R.: A re-evaluation of acetylcholine, adrenaline, noradrenaline and histamine as possible mediators of the pituitary adreno-corticotrophic activation. Endocrinology56, 248–255 (1955).
Hahn, F., Bernauer, W., Mahlstedt, J., Resch-Bollhagen, S., Beck, E.: Synergistic effects of adrenaline and mepyramine on the anaphylactic reactions of the bronchi and pulmonary vessels in the guinea pig heart-lung-preparation. Naunyn-Schmiedebergs Arch. Pharmak.267, 224–240 (1970).
Long, C. N. H., Fry, E. G.: Effect of epinephrine on adrenal cholesterol and ascorbic acid. Proc. Soc. exp. Biol. (N.Y.)59, 67–68 (1945).
Mattingly, D.: A simple fluorimetric method for the estimation of free 11-hydroxycorticoids in human plasma. J. clin. Path.15, 374–379 (1962).
Piper, P. J., Collier, H. O. J., Vane, J. R.: Release of catecholamines in the guineapig by substances involved in anaphylaxis. Nature (Lond.)213, 838–840 (1967).
Royce, P. C., Sayers, G.: Blood ACTH: Effects of ether, pentobarbital, epinephrine and pain. Endocrinology63, 794–800 (1958).
Smelik, P. G., de Wied, D.: Corticotrophin-releasing action of adrenaline, serotonin and pitressin. Experientia (Basel)14, 17–18 (1958).
Steenburg, R. W., Thomasson, B. H.: Fluorometric determination of corticosteroids in human blood: Comparison of results with the Silber-Porter method. J. clin. Endocr.24, 875–882 (1964).
Stone, S. H.: Acute and protracted anaphylactic shock in guinea pigs after subcutaneous eliciting injection of antigen. J. Immunol.82, 138–145 (1959).
Sweat, M. L.: Sulfuric acid-induced fluorescence of corticosteroids. Analyt. Chem.26, 773–776 (1954).
Thornton, M., Hughes, E. R., Kelley, V. C., Ely, R. S.: Influence of age on plasma adrenocorticosteroids in rats, rabbits, and guinea pigs. Amer. J. Physiol.202, 392–394 (1962).
Uete, T., Shimogami, A., Wasa, M.: Sulfuric acid fluorescence of various steroid hormones. J. Biochem.67, 1–7 (1970).
Vogt, M.: Observations on some conditions affecting the rate of hormone output by the suprarenal cortex. J. Physiol. (Lond.)103, 317–332 (1944).
Wilcox, H. B., Seegal, B. C.: Influence of an ethylendiamine derivative on histamine intoxication and anaphylactic shock in the intact guinea pigs and the isolated guinea pig heart. J. Immunol.44, 219–229 (1942).
Williamson, R.: Late death occurring in active anaphylaxis. J. Hyg. (Lond.)36, 588–593 (1936).
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Bernauer, W., Liebig, R. Plasma corticosteroid levels in anaphylactic shock of guinea pigs. Res. Exp. Med. 161, 233–242 (1973). https://doi.org/10.1007/BF01855050
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DOI: https://doi.org/10.1007/BF01855050