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A new experimental model of ARDS and pulmonary hypertension in the dog

  • Original Papers
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Research in Experimental Medicine

Summary

The aim of this study was to establish a stable and reproducible model of pulmonary artery hypertension with concomitant ARDS-like changes of lung function and lung morphology. In eight anesthetized and ventilated dogs, 0.01 ml/kg oleic acid (OA) was given i.v. followed by repetitive injections of 100 µm glass beads (GB) into the right atrium until a mean pulmonary artery pressure of 35–40 mm Hg was reached. Mean right ventricular (RVP) and pulmonary artery (PAP) pressures, pulmonary vascular resistance (PVR), lung compliance and resistance, PaO2, intrapulmonary shunt and colloidosmotic pressure (COP) were closely monitored for 150 min. PAP, RVP, and PVR considerably increased subsequent to OA/GB injection, and stabilized at a high level within 70 min, showing only a minimal decrease (PAP, RVP) or no change (PVR) during the following 80 min. A significant decrease of PaO2 and pulmonary compliance as well as an increase of resistance and intrapulmonary shunt were found as early as 30 min after the last embolization and they remained unchanged for 120 min. Reduction of COP suggested transcapillary leakage of macromolecules. Histology revealed an interstitial and intraalveolar edema. We conclude that the combined injection of oleic acid and glass beads provokes microvascular lung injury and results in stable pulmonary artery hypertension with concomitant ARDS-like changes of lung function. Thus, an acute model is provided in the dog allowing for the study of cardiac function in ARDS complicated by pulmonary artery hypertension.

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Authors and Affiliations

  1. Department of Experimental Surgery, University of Heidelberg, Heidelberg, Federal Republic of Germany

    B. Zwissler & K. Messmer

  2. Department of Anesthesiology, University of Munich, Medical Center Großhadern, Munich, Federal Republic of Germany

    H. Forst

  3. 2nd Department of Surgery, Miyazuki Medical College, Kihara Kiyotake-cho, Japan

    K. Ishii

Authors
  1. B. Zwissler
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  2. H. Forst
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  3. K. Ishii
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  4. K. Messmer
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Zwissler, B., Forst, H., Ishii, K. et al. A new experimental model of ARDS and pulmonary hypertension in the dog. Res. Exp. Med. 189, 427–438 (1989). https://doi.org/10.1007/BF01855010

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  • DOI: https://doi.org/10.1007/BF01855010

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