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Experimental xenogeneic kidney transplantation in closely related systems

E vivo Hemoperfusion of Kidney in the System Cat (Donor) to Dog (Recipient)

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Research in Experimental Medicine

Summary

E-vivo hemoperfusions of cat kidneys by untreated dogs were performed. Because analyses of blood and urine can be done at any time, this model has the methodical advantage above transplantation. The function time of the xenogeneic cat kidneys ranged from 10–15 hrs. In contrast to xenotransplants of closely related animals within a zoological family, which are destroyed by cellular actions, the rejection of grafts of nearly related species within a zoological order seems to be governed by humoral mechanisms. Speciesspecific but not organspecific PNAB trigger humoral and cellular factors, that lead to progressiv organ destruction. Urine and serum parameters show, that the HXR in nearly related species, compared to HXR in widely divergent species, is delayed and weakened and should therefore be distinguished.

Zusammenfassung

E vivo-Hämoperfusion von Katzennieren durch unbehandelte Hunde wurde ausgeführt. Dieses Modell erlaubt Blut- und Urinanalysen zu jeder gewünschten Zeit und besitzt so einen gewissen methodischen Vorteil gegenüber der Transplantation. Die xenogenen Nieren wurden bis zu 15 Std funktionstüchtig erhalten. Im Gegensatz zu xenogenen Transplantationen bei Tieren innerhalb einer zoologischen Familie, bei denen celluläre Abstoßungsreaktionen auftreten, scheint in diesem System von Tieren, die einer gemeinsamen Ordnung angehören, der humorale Typ der Abstoßungsreaktion vorzuherrschen. Speciesspezifische, jedoch nicht organspezifische präformierte natürliche Antikörper setzen eine Folge von humoralen und cellulären Mechanismen in Gang, die zu einer fortschreitenden Zerstörung des Organs führen. Im Urin und Serum gemessene Parameter zeigen, daß eine hyperakute xenogene Abstoßungsreaktion, die im nah verwandten Speciessystem auftritt, verglichen mit der HXAR bei entfernt verwandten Species abgeschwächt und verzögert ist und daher unterschieden werden sollte.

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Abbreviations

PNAB:

Preformed natural antibodies

HXR:

Hyperacute xenogeneic rejection

RBF:

Renal blood flow

U/P:

Urine plasma ratio

AVD:

Arterio venous difference

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Author information

Authors and Affiliations

  1. Institute Surgical Research at the Surgical Clinic of the University of Munich, Germany

    C. Hammer, C. Chaussy, G. Krebs, J. v. Scheel & W. Brendel

  2. Institut für Chirurgische Forschung, Chirurgischen Universitätsklinik, Nußbaumstraße 20, D-8000, München 2, Federal Republik of Germany

    W. Brendel

Authors
  1. C. Hammer
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  2. C. Chaussy
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  3. G. Krebs
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  4. J. v. Scheel
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  5. W. Brendel
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Members of “Sonderforschungsbereich 37: Restitution and Substitution of inner Organs”, Munich.

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Hammer, C., Chaussy, C., Krebs, G. et al. Experimental xenogeneic kidney transplantation in closely related systems. Res. Exp. Med. 160, 32–44 (1973). https://doi.org/10.1007/BF01852577

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  • DOI: https://doi.org/10.1007/BF01852577

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