Skip to main content
SpringerLink
Log in

Effects of different compositions of diets and chronic ethanol administration on proteolytic and antiproteolytic systems in the pancreatic tissue of rats

  • Original Papers
  • Published:
Research in Experimental Medicine

Summary

The effects of dietary composition in combination with chronic ethanol ingestion on pancreatic proteolytic-antiproteolytic systems were studied by submitting 192 male Wistar rats to a 12-week dietary period. The rats were fed with a standard (S), fat-rich (F), protein-rich (P), or carbohydrate-rich (C) diet after randomized division into four groups. Half of the animals in each dietary group received water and the other half a mixture of 15% (v/v) ethanol and water as their drinking solution. The C-diet caused a significant increase of BAPNA hydrolysis, which was even more pronounced in rats receiving ethanol. Casein hydrolysis was increased significantly by the C-diet, but ethanol did not have any significant effect on this. Hydrolysis of ATEE was decreased significantly by the P- and C-diets, and ethanol in combination with the S- and F-diets decreased hydrolysis of ATEE in comparison with the corresponding water-receiving groups. The trypsin-inhibiting capacity of the pancreatic tissue was significantly increased in the group receiving C- and F-diets as compared to the S-diet-receiving group of animals. It seems that the composition of the consumed diet in combination with chronic ethanol ingestion may play a role in regulating the pancreatic proteolytic-antiproteolytic system. However, these changes were not parallel. The trypsin-inhibiting capacity of pancreatic tissue was also affected by the quality of the diet.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Corring T (1977) Possible role of hydrolysis products of the dietary components in the mechanism of the exocrine pancreatic adaptation to the diet. World Rev Nutr Diet 27:132–144

    PubMed  Google Scholar 

  2. Dagorn JC, Michel R (1976) Nonparallel courses of intrapancreatic levels of exportable enzymes after a fatty meal. Proc Soc Exp Biol Med 151:608–610

    PubMed  Google Scholar 

  3. De Waele B, Kiekens R (1985) Nonparallelism of digestive enzymes in rat pancreatic hypersecretion. Digestion 31:213–219

    PubMed  Google Scholar 

  4. Goslin J, Hong SS, Magee DF, White TT (1965) Relationship between diet, ethyl alcohol consumption and some activities of the exocrine pancreas in rats. Arch Int Pharmacodyn 157:462–469

    PubMed  Google Scholar 

  5. Herva P (1970) Experimental biliary pancreatitis in dogs. Scand J Gastroenterol [Suppl] 5:1–63

    Google Scholar 

  6. Huttunen R (1973) Proteinase inhibition in plasma during experimental acute rat pancreatitis. Ann Med Exp Biol Fenn 51:199–204

    Google Scholar 

  7. Huttunen R, Korhonen LK (1973) New trypsin- and chymotrypsin-binding factor in rat plasma. Ann Med Exp Biol Fenn 51:145–150

    PubMed  Google Scholar 

  8. Izquierdo R, Sandberg L, Squillaci G, Hoppensteadt D, Walenza J, Fareed J, Prinz R (1984) Effect of protease inhibitors on coagulation abnormalities in acute pancreatitis. Ann Surg 50:317–323

    Google Scholar 

  9. Jalovaara P, Huttunen R (1977) Effect of chronic ethanol ingestion on the pancreatic proteolytic enzymes and their inhibitors in the rat. Scand J Gastroenterol 12:785–792

    PubMed  Google Scholar 

  10. Janoff A, Carp H (1982) Proteases, antiproteases, and oxidants: pathways of tissue injury during inflammation. Monogr Pathol 23:62–82

    PubMed  Google Scholar 

  11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    PubMed  Google Scholar 

  12. Miszcuk-Jamska B, Guy D, Figarella C (1983) Alpha1-proteinase inhibitor in pure pancreatic juice. Characterization of a complexed form in patients with chronic calcifying pancreatitis. Hoppe-Seylers Z Physiol Chem 364:1597–1601

    PubMed  Google Scholar 

  13. Pongpaew P, Schelp PP (1983) Albumin, prealbumin, transferrin and serum proteinase inhibitors in asymptomatic and hospitalized Thai alcohol drinkers. J Stud Alcohol 44:381–383

    PubMed  Google Scholar 

  14. Rothman SS (1974) Does pancreas secrete digestive enzymes individually or in a group? Gastroenterology 70:635–636

    Google Scholar 

  15. Rämö OJ, Kiviniemi H, Jalovaara P (1986) The composition of diet in conjunction with chronic ethanol consumption alters plasma proteinase inhibitor levels in rats. Scand J Gastroenterol 21:718–723

    PubMed  Google Scholar 

  16. Sardesai VM, Orten JM (1968) Effect of prolonged alcohol consumption in rats on pancreatic protein synthesis. J Nutr 96:241–246

    PubMed  Google Scholar 

  17. Sarles H, Lebrevil G, Tasso F, Figarella C, Demente F, Devaux MA, Fagonde B, Payan H (1971) A comparison of alcoholic pancreatitis in rat and man. Gut 12:377–388

    PubMed  Google Scholar 

  18. Singh M (1983) Effect of chronic ethanol feeding on pancreatic enzyme secretion in rats in vitro. Dig Dis Sci 28:117–123

    PubMed  Google Scholar 

  19. Singh M, LaSure M, Bockman DE (1982) Pancreatic acinar cell function and morphology in rats chronically fed an ethanol diet. Gastroenterology 82:425–434

    PubMed  Google Scholar 

  20. Wellborn JC, Alston JD, Cannon DJ, Read RC (1983) Serum proteolytic and antiproteolytic activity in acute pancreatitis. Am J Surg 146:834–837

    PubMed  Google Scholar 

  21. White TT, Allan JB (1974) Intrahepatic activation of proteases in the etiology of pancreatitis and cancer of the pancreas. Med Clin N Am 58:1305–1310

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Anatomy, University of Oulu, Kajaanintie 52A, SF-90220, Oulu 22, Finland

    O. J. Rämö & L. K. Korhonen

  2. Dept. of Surgery, University of Oulu Central Hospital, Oulu, Finland

    P. Jalovaara

Authors
  1. O. J. Rämö
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Jalovaara
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. K. Korhonen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by a grant from the Finnish Foundation for Alcohol Studies

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rämö, O.J., Jalovaara, P. & Korhonen, L.K. Effects of different compositions of diets and chronic ethanol administration on proteolytic and antiproteolytic systems in the pancreatic tissue of rats. Res. Exp. Med. 187, 95–103 (1987). https://doi.org/10.1007/BF01851971

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01851971

Key words

Search

Navigation