Summary
Estradiol and testosterone both lowered endogenous liver glycogen and at 20-fold higher doses impaired triamcinolone acetonide mediated glyconeogenesis in adult adrenalectomized male rats. Neither steroid influenced liver tyrosine transaminase although tryptophan pyrrolase activity was depressed by testosterone. Progesterone increased liver tryptophan pyrrolase but did not influence other parameters. Cortexolone did not alter either of these processes whereas cortisol induced both enzymes and, at much higher dose levels, gluconeogenesis. Binding of3H-triamcinolone acetonide to its cytoplasmic receptor in vitro was left unaffected in presence of 20-fold greater concentration of either sex steroid but almost totally abolished by cold, homologous molecules. Similar results were obtained by3H-cortisol except that estradiol partially competed for3H-cortisol binding sites even at 20-fold greater concentrations of cold estradiol. Separation on DEAE-cellulose-52 and Ultrogel 44 columns revealed binding of all steroids to macromolecules of comparable physicochemical properties although the ratios of binding to the various subpopulations of the receptor were a function of the steroid in question. These results are discussed in terms of sex steroid binding to different moieties of a complex, heterogeneous, polymorphic protein rather than inhibition of binding to the active configuration acquired in presence of an inducer.
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References
Agarwal, M. K.: Analysis of the influence of selected neurotropic agents on hepatic metabolism in relation to endotoxicosis. Biochem.Pharmacol.23, 2577 (1974)
Agarwal, M. K.: Chromatographic demonstration of mineralocorticoid-specific receptors in rat kidney. Nature254, 623 (1975)
Agarwal, M. K.: Evidence that natural vs. synthetic steroid hormones bind to physicochemically distinct cellular receptors. Biochem. Biophys. Res. Comm.73, 767 (1976)
Agarwal, M. K. (ed.): Multiple molecular forms of steroid hormone receptors. North Holland: Elsevier 1977
Agarwal, M. K. (ed.): Antihormones. North Holland: Elsevier 1979
Agarwal, M. K., Hoffman, W. W., Rosen, F.: The effect of endotoxin and thorotrast on inducible enzymes in the isolated, perfused rat liver. Biochim. Biophys. Acta177, 250 (1969)
Eisenfeld, A. J., Aten, R., Weinberger, M., Hasselbacher, G., Halpern, K., Krakoff, L.: Estrogen receptor in the mammalian liver. Science191, 862 (1975)
Gorski, H., Ganon, F.: Current models of steroid hormone action: A critique. Ann. Rev. Physiol.38, 425 (1976)
Solhanick, H. A., Kipnis, D. M., Vaude-Wiele, R. E. (eds.): Metabolic effects of gonadal hormones and contraceptive steroids. New York: Plenum Press 1969
Song, C. S., Rifkind, A. B., Gillette, P. N., Kappas, A.: Hormones and the liver. Am. J. Obs. Gynecol.105, 813 (1969)
Yamamoto, K. R.: Steroid receptors: Element for modulation of eukaryotic transcription. Ann. Rev. Biochem.45, 721 (1976)
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Supported by grants from: Délégation Générale à la Recherche Scientifique et Technique (IMB 75. 7. 0744); Institut National de la Santé et de la Recherche Médicale, and Centre National de la Recherche Scientifique
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Agarwal, M.K., Sekiya, S. & Lazar, G. Search for antiglucocorticoid activity in rat liver in vivo. Res. Exp. Med. 176, 181–192 (1979). https://doi.org/10.1007/BF01851757
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DOI: https://doi.org/10.1007/BF01851757