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Pilocarpine stimulation of exocrine pancreas secretionin vitro

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Research in Experimental Medicine

Summary

Thein vitro secretory response of mouse pancreas to pilocarpine stimulation was studied. A sigmoid-shaped concentration-response relationship was found for amylase release and concentrations of pilocarpine hydrochloride from 10−8 to 10−2 M. Higher concentrations failed to stimulate secretion. The minimal effective concentration was 10−6 M and the maximum response—approximately 3 times that of the control level during 60 min incubation—was obtained at 10−4 to 10−2 M. The secretory rate was linear during this period. The secretory response to pilocarpine was abolished by atropine and was greatly reduced under hypoxic conditions.

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Authors and Affiliations

  1. Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada

    Timo J. Nevalainen & David T. Janigan

  2. Laboratory of Elctron Microscopy, University of Turku, Kiinamyllynkatu 10, 20420, Turku 52, Finland

    Timo J. Nevalainen

Authors
  1. Timo J. Nevalainen
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  2. David T. Janigan
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Additional information

The authors wish to thank Mrs. D. Johnston and Mrs. L. Aucoin for skillful technical assistance. Supported by Medical Research Council of Canada, Grant MA-4877.

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Nevalainen, T.J., Janigan, D.T. Pilocarpine stimulation of exocrine pancreas secretionin vitro . Res. Exp. Med. 162, 161–167 (1974). https://doi.org/10.1007/BF01851614

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  • DOI: https://doi.org/10.1007/BF01851614

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