Summary
The keyproblem of hemorrhagic shock consists in decreased tissue nutrition and tissue drainage from metabolites with subsequent hypoxic cellular damage. In two groups of 50 dogs the effect of whole blood (n = 30) versus hemodilution with dextran-60 (n = 20) was evaluated from central hemodynamics, capillary blood flow and transcapillary exchange from tissue to blood in skeletal muscle by use of a double isotope technique. Following a period of hemorrhagic hypotension either all the shed blood or dextran-60 were infused. Hemodilution with dextran produced a decrease in hematocrit to 20%, lowered effeciently blood viscosity and TPR and increased cardiac output to 160% of control. Capillary blood flow and exchange were nearly doubled as compared to the control level. Retransfusion of blood caused only transient normalization with rapid deterioration in central and peripheral hemodynamics, together with an increase in blood viscosity. The viscosity depressant effect of hemodilution is discussed as key factor causing the better immediate response to dextran infusion in hemorrhagic shock.
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Abbreviations
- AVDO2 :
-
arteriovenous oxygen difference (percent saturation)
- CO:
-
cardiac output
- HCT:
-
large vessel hematocrit
- MAP:
-
mean arterial pressure
- c.v.PO2 :
-
oxygen partial pressure in centralvenous blood
- PSp:
-
permeability-surface area product
- TPR:
-
total peripheral resistance
References
Appelgren, K. L.: Effect of perfusion pressure and hematocrit on capillary flow and transport in hyperemic skeletal muscle of the dog. Microvasc. Res.4, 231 (1972).
Appelgren, K. L.: Perfusion and diffusion in shock. Acta physiol. scand., Suppl.378 (1972).
Appelgren, K. L., Lewis, D. H.: Capillary flow and capillary transport in dog skeletal muscle after induced intravascular RBC-aggregation and disaggregation. Europ. Surg. Res.2, 161 (1970).
Appelgren, K. L., Lewis, D. H.: Capillary flow and capillary transport in dog skeletal muscle in hemorrhagic shock. Europ. Surg. Res.4, 29 (1972).
Baeckström, P., Folkow, B., Löfving, B., Kovach, A. G. B., Öberg, B.: Evidence of “plugging” of the microcirculation following acute hemorrhage. In: Ditzel and Lewis, 6th Europ. Conf. Microcirculation, Aalborg 1970, pp. 16–22. Basel: Karger 1971.
Bond, T. P., Guest, M. M.: Intravascular behaviour of red cells in the microcirculation. In: Meßmer, K., Schmid-Schönbein, H.: Hemodilution. Theoretical basis and clinical application. Int. Symp. Rottach-Egern 1971, p. 46. Basel: Karger 1972.
Bond, R. F., Manley, E. S., Jr., Green, H. D.: Cutaneous and skeletal muscle vascular responses to hemorrhage and irreversible shock. Amer. J. Physiol.212, 488 (1967).
Burri, C., Allgöwer, M.: Der therapeutische Effekt verschiedener Plasmaexpander im experimentellen hämorrhagischen Schock. Chirurg36, 1 (1965).
Chien, S.: Role of sympathetic nervous system in hemorrhage. Physiol. Rev.47, 214 (1967).
Chien, S.: Blood rheology and its relation to flow resistance and transcapillary exchange with special reference to shock. Advanc. Microcirc.2, 89 (1969).
Chien, S.: Present state of blood rheology. In: Meßmer, K., Schmid-Schönbein, H.: Hemodilution. Theoretical basis and clinical application. Int. Symp. Rottach-Egern 1971, pp. 1–45. Basel: Karger 1972.
Crowell, J. W., Ford, R. G., Lewis, V. M.: Oxygen transport in hemorrhagic shock as a function of the hematocrit ratio. Amer. J. Physiol.196, 1033 (1959).
Crowell, J. W., Smith, E. E.: Oxygen deficit and irreversible hemorrhagic shock. Amer. J. Physiol.206, 313 (1964).
Cunningham, J. N., Shires, G. T., Wagner, Y.: Cellular transport defects in hemorrhagic shock. Surgery70, 215 (1971).
Dillon, J., Lynch, L. J., Jr., Meyers, R., Butcher, H. R., Moyer, C. A.: A bioassay of treatment of hemorrhagic shock. I. The role of blood, Ringer's solution with lactate, and macromolecules (dextran and hydroxyethyl starch) in the treatment of hemorrhagic shock in anesthetized dog. Arch. Surg.93, 537 (1966).
Djojosugito, A. M., Folkow, B., Öberg, B., White, S.: A comparison of blood viscosity measured in vitro and in a vascular bed. Acta physiol. scand.78, 70 (1970).
Duling, B. R., Berne, R. M.: Oxygen and the local regulation of blood flow: Possible significance of longitudinal gradients in arterial blood oxygen tension. Circulat. Res.28, Suppl. I, 65 (1971).
Fahraeus, R., Lundqvist, T.: The viscosity of the blood in narrow capillary tubes. Amer. J. Physiol.96, 562 (1931).
Foster, R. E.: Diffusion of gases. In: Handbook of Physiology, Sect. 3: Respiration, Vol. I, p. 839. Washington D. C.: Amer. Physiol. Soc. 1964.
Fronek, K., Zweifach, B. W.: Changes of splanchnic hemodynamics in hemorrhagic hypotension and endotoxemia. J. Surg. Res.11, 232 (1971).
Gaehtgens, P., Uekermann, U.: The apparent viscosity of blood in different vascular compartements of the autoperfused canine foreleg, and its variation with hematocrit. Abstracts Europ. Soc. Microcirculation 7th Conf. on Microcirculation, Aberdeen 1972, p. 18.
Gelin, L. E., Bergentz, S. E., Helander, C. G., Linder, E., Nilsson, N. J., Rudenstam, C. M.: Hemodynamic consequences from increased viscosity of blood. In: A. L. Copley, Hemorheology, p. 721. Oxford-New York: Pergamon Press 1968.
Goldstone, J., Hutchins, P. M., Schmid-Schönbein, H., Urschel, Ch., Sonnenblick, E., Wells, R.: Correlation of microvascular and rheologic factors in hemorrhagic shock. In: Ditzel and Lewis, 6th Europ. Conf. Microcirculation, Aalborg 1970, p. 150. Basel: Karger 1971.
Gosselin, R. E., Audino, L. F.: Muscle blood flow and functional capillary density evaluated by isotope clearance. Pflügers Arch.322, 197 (1971).
Gray, S. D.: Microscopic observation of skeletal muscle, vascular responses to vasopressors during severe hemorrhagic hypotension. J. Trauma12, 147 (1972).
Gruber, U. F.: Blutersatz, p. 99. Berlin-Heidelberg-New York: Springer 1968.
Gruber, U. F.: Blutersatz, p. 164. Berlin-Heidelberg-New York: Springer 1968.
Halmagyi, D. F., Kennedy, M., Goodman, A. H.: Response to dextran in posthemorrhagic shock after combined adrenergic receptor blockade. J. appl. Physiol.30, 186 (1971).
Hardaway, R. M.: The fallacy of hemorrhagic shock models in dogs. In: Ditzel and Lewis, Microcirculatory approaches to current therapeutic problems. Symposia 6th Europ. Conf. Microcirculation, Aalborg 1970, p. 24. Basel: Karger 1971.
Honig, C. R., Frierson, J. L., Nelson, C. N.: O2-transport and VO2 in resting muscle: significance for tissue-capillary exchange. Amer. J. Physiol.220, 357 (1971).
Jesch, F., Klövekorn, W. P., Sunder-Plassmann, L., Meßmer, K.: Die Bedeutung des Uptake im experimentellen hämorrhagischen Schock. Res. exp. Med.157, 267 (1972).
Kety, S. S.: Determinants of tissue oxygen tension. Fed. Proc.13, 666 (1957).
Koven, J. H., Jambunathan, G., Fung Lo, S.: The improvement of capillary transport capacity in a low flow state. Europ. Surg. Res.4, 296 (1972).
Lassen, N. A., Lindbjerg, I. F., Dahn, S.: Validity of the Xenon-133 method for measurement of muscle blood flow evaluated by simultaneous venous occlusion plethysmography. Circulat. Res.16, 287 (1965).
Lewis, D. H.: Measurement of capillary flow and capillary transport. In: Meßmer, K., Schmid-Schönbein, H., Hemodilution. Theoretical basis and clinical application, Int. Symp. Rottach-Egern 1971, p. 118. Basel: Karger 1972.
Lewis, D. H., Mellander, S.: Competitive effects of sympathetic control and tissue metabolites on resistance and capacitance vessels and capillary filtration in skeletal muscle. Acta physiol. scand.56, 162 (1962).
Meiselman, H. J., Merrill, E. W., Salzman, E. W.: Effect of dextran on rheology of human blood: low shear viscometry. J. appl. Physiol.22, 280 (1967).
Mela, L., Bacalzo, L. V., Jr., Miller, L. D.: Defective oxidative metabolism of rat liver mitochondria in hemorrhagic and endotoxin shock. Amer. J. Physiol.220, 571 (1971).
Merrill, E. W., Pelletier, G. A.: Viscosity of human blood: transition from Newtonian to non-Newtonian. J. appl. Physiol.23, 178 (1967).
Meßmer, K.: Die Grundlagen der modernen Schocktherapie. Münch. med. Wschr.112, 357 (1970).
Meßmer, K., Brendel, W.: Pathophysiologische Aspekte des hypovolämischen, kardiogenen und bakteriotoxischen Schocks. Med. Welt22, 1159 (1971).
Meßmer, K., Klövekorn, W. P., Sunder-Plassmann, L., Brendel, W.: Studies concerning the effect of trasylol in a standardized model of hemorrhagic shock in dogs. In: W. Brendel, G. L. Haberland, New aspects in trasylol therapy, p. 25. Stuttgart: Schattauer 1972.
Meßmer, K., Lewis, D. H., Sunder-Plassmann, L., Klövekorn, W. P., Mendler, N., Holper, K.: Acute normovolemic hemodilution. Changes of central hemodynamics and microcirculatory flow in skeletal muscle. Europ. Surg. Res.4, 55 (1972).
Meßmer, K., Sunder-Plassmann, L., Klövekorn, W. P., Holper, K.: Circulatory significance of hemodilution: Rheological changes and limitations. Advanc. Microcirc.4, 1 (1972).
Miller, L. D., Oski, F. A., Diaco, J. F., Sugerman, H. J., Gottlieb, A. J., Davidson, D., Delivoria-Papadopoulos, M.: The affinity of hemoglobin for oxygen: its control and in vivo significance. Surgery68, 187 (1970).
Renkin, E. M.: Exchange of substances through capillary walls. In: Wolstenholme, Knight, Circulatory and respiratory mass transport. A Ciba Foundation Symposion, p. 50. London: Churchill 1969.
Renkin, E. M.: The nutritional shunt-flow hypothesis in skeletal muscle circulation. Circulat. Res.28, Suppl. I, 21 (1971).
Replogle, R.: Hemodynamic compensation of acute changes of the hemoglobin concentration. In: K. Meßmer, H. Schmid-Schönbein, Hemodilution. Theoretical basis and clinical application. Int. Symp. Rottach-Egern 1971, p. 160. Basel: Karger 1972.
Richardson, D. R., Zweifach, B. W.: Pressure relationships in the macro- and microcirculation of the mesentery. Microvasc. Res.2, 474 (1970).
Roughton, F. J. W.: Transport of oxygen and carbon dioxide. In: Handbook of Physiology, Sect. 3: Respiration, Vol. I, p. 767. Washington D. C.: Amer. Physiol. Soc. 1964.
Schmid-Schönbein, H., Klose, J., Volger, E.: Effect of colloidal plasma substitutes on microrheology of human blood. In: K. Meßmer, H. Schmid-Schönbein, Hemodilution. Theoretical basis and clinical application. Int. Symp. Rottach-Egern 1971, p. 66. Basel: Karger 1972.
Schmid-Schönbein, H., Wells, R.: Fluid drop like transition of erythrocytes under shear. Science165, 288 (1969).
Skinner, N. S., Costin, J. C.: Interactions between oxygen, potassium and osmolality in regulation of skeletal muscle blood flow. Circulat. Res.28, 73 (1971).
Stainsly, W. N., Otis, A. B.: Blood flow, blood oxygen tension, oxygen uptake and oxygen transport in skeletal muscle. Amer. J. Physiol.206, 858 (1964).
Sunder-Plassmann, L., Klövekorn, W. P., Meßmer, K., Brendel, W.: Microcirculatory pattern in hemorrhagic shock in dogs. In: W. Brendel, G. L. Haberland, New aspects in trasylol therapy, p. 33. Stuttgart: Schattauer 1972.
Sunder-Plassmann, L., Klövekorn, W. P., Holper, K., Hase, U., Meßmer, K.: The physiological significance of acutely induced hemodilution. In: Ditzel and Lewis, 6th Europ. Conf. Microcirculation, Aalborg 1970, p. 23. Basel: Karger 1971.
Sunder-Plassmann, L., Klövekorn, W. P., Meßmer, K.: Hemodynamic and rheological changes induced by hemodilution with colloids. In: K. Meßmer, H. Schmid-Schönbein, Hemodilution. Theoretical basis and clinical application. Int. Symp. Rottach-Egern 1971, p. 184. Basel: Karger 1972.
Sunder-Plassmann, L., Klövekorn, W. P., Meßmer, K.: Veränderungen der Hämodynamik und Fließeigenschaften des Blutes bei Anwendung künstlicher Kolloide. Workshop Timmendorfer Strand: Akute Volumen- und Substitutionstherapie mit Blut, Blutbestandteilen, Plasmaersatz und Elektrolyten, p. 139. München: Lehmann 1972.
Usami, S., Chien, S.: Shear deformation of red cell ghosts. Biorheology9, 166 (1972).
Wangensteen, St. L., Glenn, Th. M., Lefer, A. M., Ferguson, W. W., Morris, J. R.: Circulatory responses to the infusion of splanchnic lysosomal enzymes. Europ. Surg. Res.4, 368 (1972).
Wells, R., Schmid-Schönbein, H.: Red cell deformation and fluidity of concentrated cell suspensions. J. appl. Physiol.27, 213 (1969).
Zweifach, B. W.: Local regulation of capillary pressure. Circulat. Res.28, 129 (1971).
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Dedicated to Prof. Dr. Dr. R. Zenker on his 70th anniversery.
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Sunder-Plassmann, L., Jesch, F., Klövekorn, W.P. et al. Limited hemodilution in hemorrhagic shock in dogs: Effects on central hemodynamics and the microcirculation in skeletal muscle. Res. Exp. Med. 159, 167–182 (1973). https://doi.org/10.1007/BF01851544
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DOI: https://doi.org/10.1007/BF01851544