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Detection of green algae (Chlorophyceae) for the diagnosis of drowning

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Abstract

The plankton test (generally, diatom test) is one of the methods available to diagnose the cause of death of submerged bodies. The solubilization method using tissue solubilizer Soluene-350 was used in this study to detect not only diatoms but also green algae, based on the fact that the solubilizer does not digest the cell walls of green algae which are made from cellulose. Detection of green algae from organs of submerged cadavers is very informative to determine drowning in fresh water, and also in cases where only few diatoms are detected in the organs.[/p]

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References

  1. Neidhart DA, Greendyke RM (1967) The significance of diatom demonstration in the diagnosis of death by drowning. Am J Clin Pathol 48: 377–382

    PubMed  Google Scholar 

  2. Udermann H, Schuhmann G (1975) Eine verbesserte Methode zum Diatomeen-Nachweis. Z Rechtsmed 76:119–122

    PubMed  Google Scholar 

  3. Peabody AJ (1977) Diatoms in forensic science. J Forensic Sci Soc 17:81–87

    Google Scholar 

  4. Peabody AJ (1980) Diatoms and drowning: a review. Med Sci Law 20: 254–261

    PubMed  Google Scholar 

  5. Ranner G, Juan H, Udermann M (1982) Zum Beweiswert von Diatomeen in Knochenmark beim Ertrinkungstod. Z Rechtsmed 88:57–65

    PubMed  Google Scholar 

  6. Auer A, Mottonen M (1988) Diatoms and drowning. Z Rechtsmed 101:87–98

    PubMed  Google Scholar 

  7. Auer A (1991) Qualitative diatom analysis as a tool to diagnose drowning. Am J Forensic Med Pathol 12: 213–218

    PubMed  Google Scholar 

  8. Ludes B, Quantin S, Coste M, Mangin P (1994) Application of a simple enzymatic digestion method for diatom detection in the diagnosis of drowning in putrified copses of diatom analysis. Int J Legal Med 107: 37–41

    PubMed  Google Scholar 

  9. Spitz WU, Schneider V (1964) The significance of diatoms in the diagnosis of death by drowning. J Forensic Sci 9:11–18

    Google Scholar 

  10. Jääskeläinen AJ (1967) Diatomeenbefunde in Wasserleichen. Eine neue Methode zur quantitativen Messung der Diatomeen im Organismus. Dtsche Z Gerichtl Med 61:41–47

    Google Scholar 

  11. Plueckhahn VD (1977) Alcohol and accidental submersion from watercraft and surrounds. Med Sci Law 17(2): 246–250

    PubMed  Google Scholar 

  12. Schellmann B, Sperl W (1979) Nachweis in Knochenmark (Femur) Nichtertrunkener. Z Rechtsmed 83:319–324

    PubMed  Google Scholar 

  13. Gylseth B, Mowe G (1979) Diatoms and lung tissue. Lancet 29: 1375

    Google Scholar 

  14. Foged N (1983) Diatoms and drowning — Once more. Forensic Sci Int 21:153–159

    PubMed  Google Scholar 

  15. Polson CJ, Gee DJ, Knight B (eds) (1985) Drowning. In: The essentials of forensic medicine. Pergamon Press, Oxford, pp 421–428

    Google Scholar 

  16. Pachar JV, Cameron JM (1992) Submersion cases: a retrospective study 1988–1990. Med Sci Law 32:15–17

    PubMed  Google Scholar 

  17. Tomita M, Fujita T, Mikami M (1981) An application of the oxygen plasma incineration to the identification of death by drowning (in Japanese). Nat Res Inst Police Sci 34:27–30

    Google Scholar 

  18. Fukui Y, Hata M, Takahashi S, Matsubara K (1980) A new method for detecting diatoms in human organs. Forensic Sci Int 16:67–74

    PubMed  Google Scholar 

  19. Matsumoto H, Fukui Y (1993) A simple method for diatom detection in drowning. Forensic Sci Int 60:91–95

    PubMed  Google Scholar 

  20. Funayama M, Aoki Y, Sebetan IM, Sagisaka K (1987) Detection of diatoms in blood by a combination of membrane filtering and chemical digestion. Forensic Sci Int 34:175–182

    PubMed  Google Scholar 

  21. Terazawa K, Takatori T (1980) Isolation of intact plankton from drowning lung tissue by centrifugation in a colloidal silica gradient. Forensic Sci Int 16:63–66

    PubMed  Google Scholar 

  22. Kobayashi M, Yamada Y, Zhang WD, Itakura Y, Nagao M, Takatori T (1993) Novel detection of plankton from lung tissue by enzymatic digestion method. Forensic Sci Int 60: 81–90

    PubMed  Google Scholar 

  23. Okada T, Ide K, Isahai I, Okada M (1983) Studies on detection method of planktons contained chlorophyll by fluorescence. Jpn J Leg Med 37:194–197

    Google Scholar 

  24. Qu J, Wang E (1992) A study on the diagnosis of drowning by examination of lung chlorophyll(a) of planktons with a spectrofluorophotometer. Forensic Sci Int 53: 149–155

    PubMed  Google Scholar 

  25. Smith IK, Lang AL (1987) Decoloration and solubilization of plant tissue prior to determination of3H,14C, and35S by liquid scintillation. Anal Biochem 164:531–536

    PubMed  Google Scholar 

  26. Mizuno T (1978) Illustrations of the freshwater plankton of Japan, Revised edn. Hoikusha, Osaka (in Japanese)

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Yoshimura, S., Yoshida, M., Okii, Y. et al. Detection of green algae (Chlorophyceae) for the diagnosis of drowning. Int J Leg Med 108, 39–42 (1995). https://doi.org/10.1007/BF01845616

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  • DOI: https://doi.org/10.1007/BF01845616

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