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Genetic Characterization of a new allele of the rabbit group b Ck allotypes

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Abstract

An inherited variant (b4v) of the rabbit kappa-chain allotype b4 is characterized by the presence of serine in place of alanine at position 121 and leucine in place of glutamine at position 124. The variant was traced through eight generations of a pedigreed rabbit family. Genetic analysis of this trait demonstrated that it is an allele of the other group b allotypes, and recent breedings have produced rabbits homozygous for this light-chain type. Two findings, other than the amino acid sequence differences, distinguish b4v from b4 in our colony. First, the level of expression of b4v in heterozygous rabbits is less than that of b4. For example, in b4b4v rabbits, approximately 30% of the preimmune IgG carries b4v. In b4vb5 animals, 46% of the IgG carries the allotype b5, although in animals of allotype b4b5, 38% of the IgG is b5. Second, retrospective analysis of some litters revealed an abnormally low frequency of b4v in male heterozygotes. However, male b4vb4v homozygotes were found at the expected frequency in prospective crosses between b4vb5 rabbits.

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References

  • Bodmer, W.F.: A new genetic model for allelism at histocompatibility and other complex loci: polymorphism for control of gene expression.Transplant. Proc. 5:1471–1475, 1973

    CAS  PubMed  Google Scholar 

  • Cazenave, P.A. and Roland, J.: Phylogenetic studies on allotype specificities of rabbit immunoglobulins.Ann. Inst. Pasteur 127c:317–332, 1976

    Google Scholar 

  • Chen, K.C.S., Kindt, T.J., and Krause, R.M.: Amino acid sequence of an allotype b4 light chain from a rabbit antibody to streptococcal carbohydrate.Proc. Natl. Acad. Sci. U.S.A. 71:1995–1998, 1974

    Article  CAS  PubMed  Google Scholar 

  • Cuatrecasas, P. and Parikh, I.: Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose.Biochemistry 11:2291–2299, 1972

    Article  CAS  PubMed  Google Scholar 

  • Dubiski, S.: Genetics and regulation of immunoglobulin allotypes.Med. Clin. North Am. 56:557–575, 1972

    CAS  PubMed  Google Scholar 

  • Ein, D.: Nonallelic behavior of the Oz groups in human immunoglobulinX chains.Proc. Natl. Acad. Sci. USA 60:982–985, 1968

    Article  CAS  PubMed  Google Scholar 

  • Farnsworth, V., Goodfliesh, R., Rodkey, S., and Hood, L.: Immunoglobulin allotypes of rabbit kappa chains: polymorphism of a control mechanism regulating closely linked duplicated genes?Proc. Natl. Acad. Sci., U.S.A. 73:1293–1296, 1976

    Article  CAS  PubMed  Google Scholar 

  • Fraser, K.J., Poulsen, R., and Haber, E.: Specific cleavage between variable and constant domains of rabbit antibody light chains by dilute acid hydrolysis.Biochemistry 11:4974–4977, 1972

    Article  CAS  PubMed  Google Scholar 

  • Gibson, D., Levanon, M., and Smithies, O.: Heterogeneity of human immunoglobulin light chains: nonallelic variation in the constant region ofX chains.Biochemistry 10:3114–3122, 1971

    Article  CAS  PubMed  Google Scholar 

  • Gilman-Sachs, A., Mage, R.G., Young, G.O., Alexander, C., and Dray, S.: Identification and genetic control of two rabbit immunoglobulin allotypes at a second light chain locus, the c locus.J. Immunol. 103:1159–1167, 1969

    CAS  PubMed  Google Scholar 

  • Gottlieb, A.B., Seide, R.K., and Kindt, T.J.: Quantitative determination of allotypic and idiotypic markers with antisera coupled to N-hydroxy-succinimide-activated Sepharose.J. Immunol. 114:51–54, 1975

    CAS  PubMed  Google Scholar 

  • Hess, M., Hilschmann, N., Rivat, L., Rivat, C., and Ropartz, C.: Isotypes in human immunoglobulin A chains.Nature (New Biol.) 234:58–61, 1971

    CAS  Google Scholar 

  • Hill, R.L., Delaney, R., Lebovitz, H.E., and Fellows, R.E.: Studies on the amino acid sequence of heavy chains from rabbit immunoglobulin G.Proc. R. Soc. B. 166:159–175, 1966

    Article  CAS  Google Scholar 

  • Kindt, T.J.: Rabbit immunoglobulin allotypes: structure, immunology, and genetics.Adv. Immunol. 21:35–86, 1975

    Article  CAS  PubMed  Google Scholar 

  • Kindt, T.J., Seide, R.K., Lachlad, H., and Thunberg, A.L.: Serologie identity of the b4 allotypic determinants present on homogeneous rabbit C chains with different N-terminal AA sequences.J. Immunol. 109:735–741, 1972

    CAS  PubMed  Google Scholar 

  • Landucci-Tosi, S., Tosi, R., and Perramon, A.: Some rabbit allotypic determinants are isotypes in hares.J. Immunol. 110:286–289, 1973

    CAS  Google Scholar 

  • Oudin, J.: Allotypy of rabbit serum proteins. I. Immunochemical analysis leading to the individualization of seven main allotypes.J. Exp. Med. 112:107–124, 1960a

    Article  CAS  PubMed  Google Scholar 

  • Oudin, J.: Allotypy of rabbit serum proteins. II. Relationships between various allotypes: Their common antigenic specificity, their distribution in a sample population: Genetic implications.J. Exp. Med. 112:125–142, 1960b

    Article  CAS  PubMed  Google Scholar 

  • Oudin, J.: Genetic regulation of immunoglobulin synthesis.J. Cell. Physiol. 67(Supp. 1):77–108, 1966

    Article  PubMed  Google Scholar 

  • Smith, L.J. and Mandy, W.J.: Expression of group b light chain allotypes in cottontail rabbits.J. Immunol. 120:624–629, 1978

    CAS  PubMed  Google Scholar 

  • Smith, L.J., Sogn, J.A., Kindt, T.J., and Mandy, W.J.: Serologie distinction between the rabbit kappa L chain allotype b4 and an allele b4v.Eur. J. Immunol, in press, 1978

  • Sogn, J.A., Hooke, P.D., Friedman, S.M., and Kindt, T.J.: Antibody avidity and allelic selection for L chain allotypes in rabbits hyperimmunized with streptococcal vaccine.J. Immunol. 113:1024–1028, 1977

    Google Scholar 

  • Sogn, J.A. and Kindt, T.J.: A genetic polymorphism in the constant region of rabbit b4 kappa chains.J. Exp. Med. 143:1475–1482, 1976

    Article  CAS  PubMed  Google Scholar 

  • Starace, V. and Querinjean, P.: The primary structure of a rat kappa Bence-Jones protein: Phylogenetic relationship of V and C region genes.J. Immunol. 115:59–62, 1975

    CAS  PubMed  Google Scholar 

  • Stemke, G.W.: Allotypic specificities A- and B-chains of rabbit gammaglobulin.Science 145:403–405, 1964

    Article  CAS  PubMed  Google Scholar 

  • Strosberg, A.D.: Multiple expression of rabbit allotypes: the tip of the iceberg?Immunogenetics 4:499–514, 1977

    Article  Google Scholar 

  • Strosberg, A.D., Hamers-Casterman, C., van der Loo, W., and Hamers, R.: A rabbit with the allotypic phenotype ala2a3/b4b5b6.J. Immunol. 113:1313–1318, 1974

    CAS  PubMed  Google Scholar 

  • Thunberg, A.L.: Studies on the genetic control of antibody variable region synthesis. Ph.D. Thesis, Rockefeller University, New York, 1974

    Google Scholar 

  • van Der Loo, W., Hamers-Casterman, C., and Hamers, R.: Phylogeny of the rabbit immunoglobulin light chain markers: distribution of determinants associated with rabbit b-locus in different logomorph families.Arch. Int. Physiol. Biochim. 84:192–193, 1976

    PubMed  Google Scholar 

  • van Der Loo, W., Hamers-Casterman, C., and Naessens, J.: Existence of two allelic forms of the rabbit immunoglobulin light chain marker b4.Arch. Intern. Physiol. Biochim. 83:203–204, 1975

    CAS  Google Scholar 

  • Wang, A., Fundenberg, H.H., and Bazin, H.: Partial amino acid sequences of k chains of rat immunoglobulins: genetic and evolutionary implications.Biochem. Genet. 14:209–223, 1976

    Article  CAS  PubMed  Google Scholar 

  • Yarmush, M.L. and Kindt, T.J.: Isolation and characterization of IgG molecules expressing latent group b allotypes from pedigreed bV rabbits.J. Exp. Med. 148:522–533, 1978

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, 20014, Bethesda, Maryland

    John A. Sogn & Thomas J. Kindt

  2. National Institutes of Health, 20014, Bethesda, Maryland

    John A. Sogn & Thomas J. Kindt

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  1. John A. Sogn
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  2. Thomas J. Kindt
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For reasons of simplicity the variant will be referred to here as b4v

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Sogn, J.A., Kindt, T.J. Genetic Characterization of a new allele of the rabbit group b Ck allotypes. Immunogenetics 7, 141–147 (1978). https://doi.org/10.1007/BF01843999

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  • DOI: https://doi.org/10.1007/BF01843999

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