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Enhanced antiproliferative activity by metastatic RAW117 lymphoma cells

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Abstract

The highly malignant/metastatic murine large cell lymphoma cell line RAW117-H10 forms 100–200 times more liver metastatic tumors than its parental counterpart cell line RAW117-P. RAW117-H10 cells, but not the less malignant/metastatic parental cells, significantly inhibited the mitogen-induced proliferation of normal syngeneic Balb/c and allogeneic ICRC mouse spleen cells. Such an inhibition also occurred when mitomycin-C treated metastatic lymphoma cells were added 24 h after initiation of culture, indicating that no competition with mitogen binding sites on the lymphocytes was necssary for inhibition of proliferation. ‘Antiproliferative’ cell surface molecules were extracted non-cytolytically from the RAW117-H10 cells using butanol. The butanol extracts from the metastatic RAW117-H10 cells also inhibited the mitogen-induced proliferation and natural killer (NK) cell-mediated cytotoxicity of normal spleen cells. Our results indicate that these ‘antiproliferative’ cell surface molecules of metastatic murine RAW117-H10 lymphoma cells may have important role(s) in tumor-mediated host immunosuppression.

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Author notes

  1. H. M. Gharpure

    Present address: Span Research Center, 175 New Industrial Estate, 394210, Udhna, Surat, India

Authors and Affiliations

  1. Department of Anatomy, University of Nebraska Medical Center Omaha, 68198-6395, Nebraska, USA

    S. S. Joshi & J. G. Sharp

  2. Department of Pathology & Microbiology, University of Nebraska Medical Center Omaha, 68198-6395, Nebraska, USA

    S. J. O'connor & D. D. Weisenburger

  3. Department of Microbiology and Immunology, Indiana University School of Medicine, 46408, Gary, Indiana, USA

    H. M. Gharpure

  4. Department of Cancer Biology, Pfizer Central Research, Eastern Point Road, 06340, Groton, Connecticut, USA

    K. W. Brunson

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  1. S. S. Joshi
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Joshi, S.S., O'connor, S.J., Weisenburger, D.D. et al. Enhanced antiproliferative activity by metastatic RAW117 lymphoma cells. Clin Exp Metast 9, 27–37 (1991). https://doi.org/10.1007/BF01831707

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  • DOI: https://doi.org/10.1007/BF01831707

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